Protocol for the processing and downstream analysis of phosphoproteomic data with PhosR

Analysis of phosphoproteomic data requires advanced computational methodologies. To this end, we developed PhosR, a set of tools and methodologies implemented in R to allow the comprehensive analysis of phosphoproteomic data. PhosR enables processing steps such as imputation, normalization, and func...

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Detalles Bibliográficos
Autores principales: Kim, Hani Jieun, Kim, Taiyun, Xiao, Di, Yang, Pengyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190506/
https://www.ncbi.nlm.nih.gov/pubmed/34151303
http://dx.doi.org/10.1016/j.xpro.2021.100585
Descripción
Sumario:Analysis of phosphoproteomic data requires advanced computational methodologies. To this end, we developed PhosR, a set of tools and methodologies implemented in R to allow the comprehensive analysis of phosphoproteomic data. PhosR enables processing steps such as imputation, normalization, and functional analysis such as kinase activity inference and signalome construction. Together, PhosR facilitates interpretation and discovery from large-scale phosphoproteomic data sets. For complete details on the use and execution of this protocol, please refer to Kim et al. (2021).

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