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Optimized protocol for the identification of lipid droplet proteomes using proximity labeling proteomics in cultured human cells

Lipid droplets are endoplasmic reticulum-derived neutral lipid storage organelles that play critical roles in cellular lipid and energy homeostasis. Here, we present a protocol for the identification of high-confidence lipid droplet proteomes in a cell culture model. This approach overcomes limitati...

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Detalles Bibliográficos
Autores principales: Peterson, Clark W.H., Deol, Kirandeep K., To, Milton, Olzmann, James A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190507/
https://www.ncbi.nlm.nih.gov/pubmed/34151299
http://dx.doi.org/10.1016/j.xpro.2021.100579

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