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Optimized protocol for the identification of lipid droplet proteomes using proximity labeling proteomics in cultured human cells
Lipid droplets are endoplasmic reticulum-derived neutral lipid storage organelles that play critical roles in cellular lipid and energy homeostasis. Here, we present a protocol for the identification of high-confidence lipid droplet proteomes in a cell culture model. This approach overcomes limitati...
Autores principales: | Peterson, Clark W.H., Deol, Kirandeep K., To, Milton, Olzmann, James A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190507/ https://www.ncbi.nlm.nih.gov/pubmed/34151299 http://dx.doi.org/10.1016/j.xpro.2021.100579 |
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