Generation and validation of APOE knockout human iPSC-derived cerebral organoids

Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with the pathogenesis of Alzheimer's disease (AD). Here, we describe a protocol for efficient knockout of APOE in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system. We obtain homozygous...

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Autores principales: Martens, Yuka A., Xu, Siming, Tait, Richard, Li, Gary, Zhao, Xinping C., Lu, Wenyan, Liu, Chia-Chen, Kanekiyo, Takahisa, Bu, Guojun, Zhao, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190508/
https://www.ncbi.nlm.nih.gov/pubmed/34151296
http://dx.doi.org/10.1016/j.xpro.2021.100571
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author Martens, Yuka A.
Xu, Siming
Tait, Richard
Li, Gary
Zhao, Xinping C.
Lu, Wenyan
Liu, Chia-Chen
Kanekiyo, Takahisa
Bu, Guojun
Zhao, Jing
author_facet Martens, Yuka A.
Xu, Siming
Tait, Richard
Li, Gary
Zhao, Xinping C.
Lu, Wenyan
Liu, Chia-Chen
Kanekiyo, Takahisa
Bu, Guojun
Zhao, Jing
author_sort Martens, Yuka A.
collection PubMed
description Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with the pathogenesis of Alzheimer's disease (AD). Here, we describe a protocol for efficient knockout of APOE in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system. We obtain homozygous APOE knockout (APOE(-/-)) iPSCs and further validate the deficiency of apoE in iPSC-derived cerebral organoids. APOE(-/-) cerebral organoids can serve as a useful tool to study apoE functions and apoE-related pathogenic mechanisms in AD. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).
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spelling pubmed-81905082021-06-17 Generation and validation of APOE knockout human iPSC-derived cerebral organoids Martens, Yuka A. Xu, Siming Tait, Richard Li, Gary Zhao, Xinping C. Lu, Wenyan Liu, Chia-Chen Kanekiyo, Takahisa Bu, Guojun Zhao, Jing STAR Protoc Protocol Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with the pathogenesis of Alzheimer's disease (AD). Here, we describe a protocol for efficient knockout of APOE in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system. We obtain homozygous APOE knockout (APOE(-/-)) iPSCs and further validate the deficiency of apoE in iPSC-derived cerebral organoids. APOE(-/-) cerebral organoids can serve as a useful tool to study apoE functions and apoE-related pathogenic mechanisms in AD. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020). Elsevier 2021-06-05 /pmc/articles/PMC8190508/ /pubmed/34151296 http://dx.doi.org/10.1016/j.xpro.2021.100571 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Martens, Yuka A.
Xu, Siming
Tait, Richard
Li, Gary
Zhao, Xinping C.
Lu, Wenyan
Liu, Chia-Chen
Kanekiyo, Takahisa
Bu, Guojun
Zhao, Jing
Generation and validation of APOE knockout human iPSC-derived cerebral organoids
title Generation and validation of APOE knockout human iPSC-derived cerebral organoids
title_full Generation and validation of APOE knockout human iPSC-derived cerebral organoids
title_fullStr Generation and validation of APOE knockout human iPSC-derived cerebral organoids
title_full_unstemmed Generation and validation of APOE knockout human iPSC-derived cerebral organoids
title_short Generation and validation of APOE knockout human iPSC-derived cerebral organoids
title_sort generation and validation of apoe knockout human ipsc-derived cerebral organoids
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190508/
https://www.ncbi.nlm.nih.gov/pubmed/34151296
http://dx.doi.org/10.1016/j.xpro.2021.100571
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