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Lactobacillus reuteri Colonisation of Extremely Preterm Infants in a Randomised Placebo-Controlled Trial

Lactobacillus reuteri DSM 17938 supplementation reduces morbidities in very low birth weight infants (<1500 g), while the effect on extremely low birth weight infants (ELBW, <1000 g) is still questioned. In a randomised placebo-controlled trial (ClinicalTrials.gov ID NCT01603368), head growth,...

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Autores principales: Spreckels, Johanne E., Wejryd, Erik, Marchini, Giovanna, Jonsson, Baldvin, de Vries, Dylan H., Jenmalm, Maria C., Landberg, Eva, Sverremark-Ekström, Eva, Martí, Magalí, Abrahamsson, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190634/
https://www.ncbi.nlm.nih.gov/pubmed/33923278
http://dx.doi.org/10.3390/microorganisms9050915
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author Spreckels, Johanne E.
Wejryd, Erik
Marchini, Giovanna
Jonsson, Baldvin
de Vries, Dylan H.
Jenmalm, Maria C.
Landberg, Eva
Sverremark-Ekström, Eva
Martí, Magalí
Abrahamsson, Thomas
author_facet Spreckels, Johanne E.
Wejryd, Erik
Marchini, Giovanna
Jonsson, Baldvin
de Vries, Dylan H.
Jenmalm, Maria C.
Landberg, Eva
Sverremark-Ekström, Eva
Martí, Magalí
Abrahamsson, Thomas
author_sort Spreckels, Johanne E.
collection PubMed
description Lactobacillus reuteri DSM 17938 supplementation reduces morbidities in very low birth weight infants (<1500 g), while the effect on extremely low birth weight infants (ELBW, <1000 g) is still questioned. In a randomised placebo-controlled trial (ClinicalTrials.gov ID NCT01603368), head growth, but not feeding tolerance or morbidities, improved in L. reuteri-supplemented preterm ELBW infants. Here, we investigate colonisation with the probiotic strain in preterm ELBW infants who received L. reuteri DSM 17938 or a placebo from birth to postmenstrual week (PMW) 36. Quantitative PCR was used on 582 faecal DNA samples collected from 132 ELBW infants at one, two, three, and four weeks, at PMW 36, and at two years of age. Human milk oligosaccharides were measured in 31 milk samples at two weeks postpartum. At least 86% of the ELBW infants in the L. reuteri group were colonised with the probiotic strain during the neonatal period, despite low gestational age, high antibiotic pressure, and independent of infant feeding mode. Higher concentrations of lacto-N-tetraose, sialyl-lacto-N-neotetraose c, and 6′-sialyllactose in mother’s milk weakly correlated with lower L. reuteri abundance. Within the L. reuteri group, higher L. reuteri abundance weakly correlated with a shorter time to reach full enteral feeding. Female sex and L. reuteri colonisation improved head growth from birth to four weeks of age. In conclusion, L. reuteri DSM 17938 supplementation leads to successful colonisation in ELBW infants.
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spelling pubmed-81906342021-06-11 Lactobacillus reuteri Colonisation of Extremely Preterm Infants in a Randomised Placebo-Controlled Trial Spreckels, Johanne E. Wejryd, Erik Marchini, Giovanna Jonsson, Baldvin de Vries, Dylan H. Jenmalm, Maria C. Landberg, Eva Sverremark-Ekström, Eva Martí, Magalí Abrahamsson, Thomas Microorganisms Article Lactobacillus reuteri DSM 17938 supplementation reduces morbidities in very low birth weight infants (<1500 g), while the effect on extremely low birth weight infants (ELBW, <1000 g) is still questioned. In a randomised placebo-controlled trial (ClinicalTrials.gov ID NCT01603368), head growth, but not feeding tolerance or morbidities, improved in L. reuteri-supplemented preterm ELBW infants. Here, we investigate colonisation with the probiotic strain in preterm ELBW infants who received L. reuteri DSM 17938 or a placebo from birth to postmenstrual week (PMW) 36. Quantitative PCR was used on 582 faecal DNA samples collected from 132 ELBW infants at one, two, three, and four weeks, at PMW 36, and at two years of age. Human milk oligosaccharides were measured in 31 milk samples at two weeks postpartum. At least 86% of the ELBW infants in the L. reuteri group were colonised with the probiotic strain during the neonatal period, despite low gestational age, high antibiotic pressure, and independent of infant feeding mode. Higher concentrations of lacto-N-tetraose, sialyl-lacto-N-neotetraose c, and 6′-sialyllactose in mother’s milk weakly correlated with lower L. reuteri abundance. Within the L. reuteri group, higher L. reuteri abundance weakly correlated with a shorter time to reach full enteral feeding. Female sex and L. reuteri colonisation improved head growth from birth to four weeks of age. In conclusion, L. reuteri DSM 17938 supplementation leads to successful colonisation in ELBW infants. MDPI 2021-04-24 /pmc/articles/PMC8190634/ /pubmed/33923278 http://dx.doi.org/10.3390/microorganisms9050915 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Spreckels, Johanne E.
Wejryd, Erik
Marchini, Giovanna
Jonsson, Baldvin
de Vries, Dylan H.
Jenmalm, Maria C.
Landberg, Eva
Sverremark-Ekström, Eva
Martí, Magalí
Abrahamsson, Thomas
Lactobacillus reuteri Colonisation of Extremely Preterm Infants in a Randomised Placebo-Controlled Trial
title Lactobacillus reuteri Colonisation of Extremely Preterm Infants in a Randomised Placebo-Controlled Trial
title_full Lactobacillus reuteri Colonisation of Extremely Preterm Infants in a Randomised Placebo-Controlled Trial
title_fullStr Lactobacillus reuteri Colonisation of Extremely Preterm Infants in a Randomised Placebo-Controlled Trial
title_full_unstemmed Lactobacillus reuteri Colonisation of Extremely Preterm Infants in a Randomised Placebo-Controlled Trial
title_short Lactobacillus reuteri Colonisation of Extremely Preterm Infants in a Randomised Placebo-Controlled Trial
title_sort lactobacillus reuteri colonisation of extremely preterm infants in a randomised placebo-controlled trial
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190634/
https://www.ncbi.nlm.nih.gov/pubmed/33923278
http://dx.doi.org/10.3390/microorganisms9050915
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