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Expanding the SiMPl Plasmid Toolbox for Use with Spectinomycin/Streptomycin
[Image: see text] We recently developed the SiMPl plasmid toolbox, which is constituted by pairs of plasmids, generically indicated as pSiMPl(x)_N and pSiMPl(x)_C, which can be stably maintained in Escherichia coli with a single antibiotic x. The method exploits the split intein gp41–1 to reconstitu...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190784/ https://www.ncbi.nlm.nih.gov/pubmed/34124437 http://dx.doi.org/10.1021/acsomega.1c00649 |