An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells

Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, in vit...

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Detalles Bibliográficos
Autores principales: Baglia, Regina A., Mills, Kira R., Mitra, Koushambi, Tutol, Jasmine N., Ball, Darby, Page, Kierstin M., Kallu, Jyothi, Gottipolu, Sriharika, D’Arcy, Sheena, Nielsen, Steven O., Dodani, Sheel C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190907/
https://www.ncbi.nlm.nih.gov/pubmed/34212150
http://dx.doi.org/10.1039/d0cb00231c
Descripción
Sumario:Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, in vitro enzymology, and biological application of STS-3, an activity-based fluorescent sensor for the SULT1A1 isoform.

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