An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells

Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, in vit...

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Detalles Bibliográficos
Autores principales: Baglia, Regina A., Mills, Kira R., Mitra, Koushambi, Tutol, Jasmine N., Ball, Darby, Page, Kierstin M., Kallu, Jyothi, Gottipolu, Sriharika, D’Arcy, Sheena, Nielsen, Steven O., Dodani, Sheel C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190907/
https://www.ncbi.nlm.nih.gov/pubmed/34212150
http://dx.doi.org/10.1039/d0cb00231c
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author Baglia, Regina A.
Mills, Kira R.
Mitra, Koushambi
Tutol, Jasmine N.
Ball, Darby
Page, Kierstin M.
Kallu, Jyothi
Gottipolu, Sriharika
D’Arcy, Sheena
Nielsen, Steven O.
Dodani, Sheel C.
author_facet Baglia, Regina A.
Mills, Kira R.
Mitra, Koushambi
Tutol, Jasmine N.
Ball, Darby
Page, Kierstin M.
Kallu, Jyothi
Gottipolu, Sriharika
D’Arcy, Sheena
Nielsen, Steven O.
Dodani, Sheel C.
author_sort Baglia, Regina A.
collection PubMed
description Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, in vitro enzymology, and biological application of STS-3, an activity-based fluorescent sensor for the SULT1A1 isoform.
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spelling pubmed-81909072021-06-29 An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells Baglia, Regina A. Mills, Kira R. Mitra, Koushambi Tutol, Jasmine N. Ball, Darby Page, Kierstin M. Kallu, Jyothi Gottipolu, Sriharika D’Arcy, Sheena Nielsen, Steven O. Dodani, Sheel C. RSC Chem Biol Chemistry Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, in vitro enzymology, and biological application of STS-3, an activity-based fluorescent sensor for the SULT1A1 isoform. RSC 2021-03-15 /pmc/articles/PMC8190907/ /pubmed/34212150 http://dx.doi.org/10.1039/d0cb00231c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Baglia, Regina A.
Mills, Kira R.
Mitra, Koushambi
Tutol, Jasmine N.
Ball, Darby
Page, Kierstin M.
Kallu, Jyothi
Gottipolu, Sriharika
D’Arcy, Sheena
Nielsen, Steven O.
Dodani, Sheel C.
An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells
title An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells
title_full An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells
title_fullStr An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells
title_full_unstemmed An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells
title_short An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells
title_sort activity-based fluorescent sensor for the detection of the phenol sulfotransferase sult1a1 in living cells
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190907/
https://www.ncbi.nlm.nih.gov/pubmed/34212150
http://dx.doi.org/10.1039/d0cb00231c
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