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Dengue virus 2 capsid protein chaperones the strand displacement of 5′-3′ cyclization sequences
By virtue of its chaperone activity, the capsid protein of dengue virus strain 2 (DENV2C) promotes nucleic acid structural rearrangements. However, the role of DENV2C during the interaction of RNA elements involved in stabilizing the 5′-3′ panhandle structure of DENV RNA is still unclear. Therefore,...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191770/ https://www.ncbi.nlm.nih.gov/pubmed/34037793 http://dx.doi.org/10.1093/nar/gkab379 |
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author | Yong, Xin Ee Palur, V Raghuvamsi Anand, Ganesh S Wohland, Thorsten Sharma, Kamal K |
author_facet | Yong, Xin Ee Palur, V Raghuvamsi Anand, Ganesh S Wohland, Thorsten Sharma, Kamal K |
author_sort | Yong, Xin Ee |
collection | PubMed |
description | By virtue of its chaperone activity, the capsid protein of dengue virus strain 2 (DENV2C) promotes nucleic acid structural rearrangements. However, the role of DENV2C during the interaction of RNA elements involved in stabilizing the 5′-3′ panhandle structure of DENV RNA is still unclear. Therefore, we determined how DENV2C affects structural functionality of the capsid-coding region hairpin element (cHP) during annealing and strand displacement of the 9-nt cyclization sequence (5CS) and its complementary 3CS. cHP has two distinct functions: a role in translation start codon selection and a role in RNA synthesis. Our results showed that cHP impedes annealing between 5CS and 3CS. Although DENV2C does not modulate structural functionality of cHP, it accelerates annealing and specifically promotes strand displacement of 3CS during 5′-3′ panhandle formation. Furthermore, DENV2C exerts its chaperone activity by favouring one of the active conformations of cHP. Based on our results, we propose mechanisms for annealing and strand displacement involving cHP. Thus, our results provide mechanistic insights into how DENV2C regulates RNA synthesis by modulating essential RNA elements in the capsid-coding region, that in turn allow for DENV replication. |
format | Online Article Text |
id | pubmed-8191770 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-81917702021-06-11 Dengue virus 2 capsid protein chaperones the strand displacement of 5′-3′ cyclization sequences Yong, Xin Ee Palur, V Raghuvamsi Anand, Ganesh S Wohland, Thorsten Sharma, Kamal K Nucleic Acids Res RNA and RNA-protein complexes By virtue of its chaperone activity, the capsid protein of dengue virus strain 2 (DENV2C) promotes nucleic acid structural rearrangements. However, the role of DENV2C during the interaction of RNA elements involved in stabilizing the 5′-3′ panhandle structure of DENV RNA is still unclear. Therefore, we determined how DENV2C affects structural functionality of the capsid-coding region hairpin element (cHP) during annealing and strand displacement of the 9-nt cyclization sequence (5CS) and its complementary 3CS. cHP has two distinct functions: a role in translation start codon selection and a role in RNA synthesis. Our results showed that cHP impedes annealing between 5CS and 3CS. Although DENV2C does not modulate structural functionality of cHP, it accelerates annealing and specifically promotes strand displacement of 3CS during 5′-3′ panhandle formation. Furthermore, DENV2C exerts its chaperone activity by favouring one of the active conformations of cHP. Based on our results, we propose mechanisms for annealing and strand displacement involving cHP. Thus, our results provide mechanistic insights into how DENV2C regulates RNA synthesis by modulating essential RNA elements in the capsid-coding region, that in turn allow for DENV replication. Oxford University Press 2021-05-25 /pmc/articles/PMC8191770/ /pubmed/34037793 http://dx.doi.org/10.1093/nar/gkab379 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | RNA and RNA-protein complexes Yong, Xin Ee Palur, V Raghuvamsi Anand, Ganesh S Wohland, Thorsten Sharma, Kamal K Dengue virus 2 capsid protein chaperones the strand displacement of 5′-3′ cyclization sequences |
title | Dengue virus 2 capsid protein chaperones the strand displacement of 5′-3′ cyclization sequences |
title_full | Dengue virus 2 capsid protein chaperones the strand displacement of 5′-3′ cyclization sequences |
title_fullStr | Dengue virus 2 capsid protein chaperones the strand displacement of 5′-3′ cyclization sequences |
title_full_unstemmed | Dengue virus 2 capsid protein chaperones the strand displacement of 5′-3′ cyclization sequences |
title_short | Dengue virus 2 capsid protein chaperones the strand displacement of 5′-3′ cyclization sequences |
title_sort | dengue virus 2 capsid protein chaperones the strand displacement of 5′-3′ cyclization sequences |
topic | RNA and RNA-protein complexes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191770/ https://www.ncbi.nlm.nih.gov/pubmed/34037793 http://dx.doi.org/10.1093/nar/gkab379 |
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