Minimized combinatorial CRISPR screens identify genetic interactions in autophagy

Combinatorial CRISPR-Cas screens have advanced the mapping of genetic interactions, but their experimental scale limits the number of targetable gene combinations. Here, we describe 3Cs multiplexing, a rapid and scalable method to generate highly diverse and uniformly distributed combinatorial CRISP...

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Detalles Bibliográficos
Autores principales: Diehl, Valentina, Wegner, Martin, Grumati, Paolo, Husnjak, Koraljka, Schaubeck, Simone, Gubas, Andrea, Shah, Varun Jayeshkumar, Polat, Ibrahim H, Langschied, Felix, Prieto-Garcia, Cristian, Müller, Konstantin, Kalousi, Alkmini, Ebersberger, Ingo, Brandts, Christian H, Dikic, Ivan, Kaulich, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Genomics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191801/
https://www.ncbi.nlm.nih.gov/pubmed/33956155
http://dx.doi.org/10.1093/nar/gkab309

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