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Genetic diversity of Venturia inaequalis isolates (Apple scab) in China and U.K. determined by SSR markers

Apple scab caused by Venturia inaequalis is a serious disease of cultivated apple worldwide. In this study, we collected 132 V. inaequalis isolates from Shaanxi, Gansu, Xinjiang, and the U.K. and analyzed their genetic diversity by using 13 microsatellite markers. Cluster analysis based on populatio...

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Autores principales: Li, Xiancheng, Tao, Fei, Fan, Sanhong, Li, Haiyuan, Yang, Jiarong, Gao, Liqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191898/
https://www.ncbi.nlm.nih.gov/pubmed/34111167
http://dx.doi.org/10.1371/journal.pone.0252865
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author Li, Xiancheng
Tao, Fei
Fan, Sanhong
Li, Haiyuan
Yang, Jiarong
Gao, Liqiang
author_facet Li, Xiancheng
Tao, Fei
Fan, Sanhong
Li, Haiyuan
Yang, Jiarong
Gao, Liqiang
author_sort Li, Xiancheng
collection PubMed
description Apple scab caused by Venturia inaequalis is a serious disease of cultivated apple worldwide. In this study, we collected 132 V. inaequalis isolates from Shaanxi, Gansu, Xinjiang, and the U.K. and analyzed their genetic diversity by using 13 microsatellite markers. Cluster analysis based on population structure and genetic distances suggested high similarity among the four regions. Population differentiation values ranged from 0.044 to 0.155, indicating there is a high level of kinship among the four regions. All isolates could be divided into 5 lineages with a 0.76 similarity coefficient. Among the four regions, Shaanxi had only one lineage, Group II; Gansu had four lineages, Group I, Group II, Group IV, and Group V; Xinjiang had all five lineages, Group I, Group II, Group III, Group IV, and Group V; and the U.K. had three lineages, Group I, Group II and Group IV. High molecular variance was detected for populations in the four regions, with 91% of the variance occurring within the populations and 9% among the populations. Structure analysis there are three common ancestors of these four regions. The results of the present study shed light on the genetic diversity of V. inaequalis in Shaanxi, Gansu and Xinjiang, which will lead to the development of more effective management strategies and new resistant apple cultivars through molecular marker-assisted selection.
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spelling pubmed-81918982021-06-10 Genetic diversity of Venturia inaequalis isolates (Apple scab) in China and U.K. determined by SSR markers Li, Xiancheng Tao, Fei Fan, Sanhong Li, Haiyuan Yang, Jiarong Gao, Liqiang PLoS One Research Article Apple scab caused by Venturia inaequalis is a serious disease of cultivated apple worldwide. In this study, we collected 132 V. inaequalis isolates from Shaanxi, Gansu, Xinjiang, and the U.K. and analyzed their genetic diversity by using 13 microsatellite markers. Cluster analysis based on population structure and genetic distances suggested high similarity among the four regions. Population differentiation values ranged from 0.044 to 0.155, indicating there is a high level of kinship among the four regions. All isolates could be divided into 5 lineages with a 0.76 similarity coefficient. Among the four regions, Shaanxi had only one lineage, Group II; Gansu had four lineages, Group I, Group II, Group IV, and Group V; Xinjiang had all five lineages, Group I, Group II, Group III, Group IV, and Group V; and the U.K. had three lineages, Group I, Group II and Group IV. High molecular variance was detected for populations in the four regions, with 91% of the variance occurring within the populations and 9% among the populations. Structure analysis there are three common ancestors of these four regions. The results of the present study shed light on the genetic diversity of V. inaequalis in Shaanxi, Gansu and Xinjiang, which will lead to the development of more effective management strategies and new resistant apple cultivars through molecular marker-assisted selection. Public Library of Science 2021-06-10 /pmc/articles/PMC8191898/ /pubmed/34111167 http://dx.doi.org/10.1371/journal.pone.0252865 Text en © 2021 Li et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Li, Xiancheng
Tao, Fei
Fan, Sanhong
Li, Haiyuan
Yang, Jiarong
Gao, Liqiang
Genetic diversity of Venturia inaequalis isolates (Apple scab) in China and U.K. determined by SSR markers
title Genetic diversity of Venturia inaequalis isolates (Apple scab) in China and U.K. determined by SSR markers
title_full Genetic diversity of Venturia inaequalis isolates (Apple scab) in China and U.K. determined by SSR markers
title_fullStr Genetic diversity of Venturia inaequalis isolates (Apple scab) in China and U.K. determined by SSR markers
title_full_unstemmed Genetic diversity of Venturia inaequalis isolates (Apple scab) in China and U.K. determined by SSR markers
title_short Genetic diversity of Venturia inaequalis isolates (Apple scab) in China and U.K. determined by SSR markers
title_sort genetic diversity of venturia inaequalis isolates (apple scab) in china and u.k. determined by ssr markers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191898/
https://www.ncbi.nlm.nih.gov/pubmed/34111167
http://dx.doi.org/10.1371/journal.pone.0252865
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