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Detection of SARS-CoV-2 using qRT-PCR in saliva obtained from asymptomatic or mild COVID-19 patients, comparative analysis with matched nasopharyngeal samples

OBJECTIVES: The accurate detection of severe acute respiratory syndrome–coronavirus 2 (SARS-CoV-2) is essential for the diagnosis of coronavirus disease 2019 (COVID-19). We compared the quantitative RT-PCR results between nasopharyngeal swabs and saliva specimens. METHODS: A COVID-19 outbreak occurr...

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Detalles Bibliográficos
Autores principales: Ota, Kenji, Yanagihara, Katsunori, Sasaki, Daisuke, Kaku, Norihito, Uno, Naoki, Sakamoto, Kei, Kosai, Kosuke, Miyazaki, Taiga, Hasegawa, Hiroo, Fujita, Ayumi, Tashiro, Masato, Tanaka, Takeshi, Izumikawa, Koichi, Ariyoshi, Koya, Mukae, Hiroshi, Yasuda, Jiro, Morita, Kouichi, Kohno, Shigeru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191987/
https://www.ncbi.nlm.nih.gov/pubmed/34111203
http://dx.doi.org/10.1371/journal.pone.0252964
Descripción
Sumario:OBJECTIVES: The accurate detection of severe acute respiratory syndrome–coronavirus 2 (SARS-CoV-2) is essential for the diagnosis of coronavirus disease 2019 (COVID-19). We compared the quantitative RT-PCR results between nasopharyngeal swabs and saliva specimens. METHODS: A COVID-19 outbreak occurred on a cruise ship at Nagasaki port, Japan. We obtained 123 nasopharyngeal swabs and saliva each from asymptomatic or mild patients in the late phase of infection. RESULTS: The intervals from the diagnosis to the sampling were 25.5 days for nasopharyngeal swabs and 28.9 days for saliva. The positive rate was 19.5% (24/123) for nasopharyngeal swabs and 38.2% (47/123) for saliva (P = 0.48). The quantified viral copies (mean ± SEM copies/5 μl) were 9.3±2.6 in nasopharyngeal swabs and 920±850 in saliva (P = 0.0006). CONCLUSIONS: The advantages of saliva specimens include positive rate improvement and accurate viral load detection. Saliva may be used as a reliable sample for SARS-CoV-2 detection.