Cargando…

All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors

Voltage imaging and “all-optical electrophysiology” in human induced pluripotent stem cell (hiPSC)-derived neurons have opened unprecedented opportunities for high-throughput phenotyping of activity in neurons possessing unique genetic backgrounds of individual patients. While prior all-optical elec...

Descripción completa

Detalles Bibliográficos
Autores principales: Puppo, Francesca, Sadegh, Sanaz, Trujillo, Cleber A., Thunemann, Martin, Campbell, Evan P., Vandenberghe, Matthieu, Shan, Xiwei, Akkouh, Ibrahim A., Miller, Evan W., Bloodgood, Brenda L., Silva, Gabriel A., Dale, Anders M., Einevoll, Gaute T., Djurovic, Srdjan, Andreassen, Ole A., Muotri, Alysson R., Devor, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193062/
https://www.ncbi.nlm.nih.gov/pubmed/34122014
http://dx.doi.org/10.3389/fncel.2021.671549
_version_ 1783706174702485504
author Puppo, Francesca
Sadegh, Sanaz
Trujillo, Cleber A.
Thunemann, Martin
Campbell, Evan P.
Vandenberghe, Matthieu
Shan, Xiwei
Akkouh, Ibrahim A.
Miller, Evan W.
Bloodgood, Brenda L.
Silva, Gabriel A.
Dale, Anders M.
Einevoll, Gaute T.
Djurovic, Srdjan
Andreassen, Ole A.
Muotri, Alysson R.
Devor, Anna
author_facet Puppo, Francesca
Sadegh, Sanaz
Trujillo, Cleber A.
Thunemann, Martin
Campbell, Evan P.
Vandenberghe, Matthieu
Shan, Xiwei
Akkouh, Ibrahim A.
Miller, Evan W.
Bloodgood, Brenda L.
Silva, Gabriel A.
Dale, Anders M.
Einevoll, Gaute T.
Djurovic, Srdjan
Andreassen, Ole A.
Muotri, Alysson R.
Devor, Anna
author_sort Puppo, Francesca
collection PubMed
description Voltage imaging and “all-optical electrophysiology” in human induced pluripotent stem cell (hiPSC)-derived neurons have opened unprecedented opportunities for high-throughput phenotyping of activity in neurons possessing unique genetic backgrounds of individual patients. While prior all-optical electrophysiology studies relied on genetically encoded voltage indicators, here, we demonstrate an alternative protocol using a synthetic voltage sensor and genetically encoded optogenetic actuator that generate robust and reproducible results. We demonstrate the functionality of this method by measuring spontaneous and evoked activity in three independent hiPSC-derived neuronal cell lines with distinct genetic backgrounds.
format Online
Article
Text
id pubmed-8193062
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-81930622021-06-12 All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors Puppo, Francesca Sadegh, Sanaz Trujillo, Cleber A. Thunemann, Martin Campbell, Evan P. Vandenberghe, Matthieu Shan, Xiwei Akkouh, Ibrahim A. Miller, Evan W. Bloodgood, Brenda L. Silva, Gabriel A. Dale, Anders M. Einevoll, Gaute T. Djurovic, Srdjan Andreassen, Ole A. Muotri, Alysson R. Devor, Anna Front Cell Neurosci Neuroscience Voltage imaging and “all-optical electrophysiology” in human induced pluripotent stem cell (hiPSC)-derived neurons have opened unprecedented opportunities for high-throughput phenotyping of activity in neurons possessing unique genetic backgrounds of individual patients. While prior all-optical electrophysiology studies relied on genetically encoded voltage indicators, here, we demonstrate an alternative protocol using a synthetic voltage sensor and genetically encoded optogenetic actuator that generate robust and reproducible results. We demonstrate the functionality of this method by measuring spontaneous and evoked activity in three independent hiPSC-derived neuronal cell lines with distinct genetic backgrounds. Frontiers Media S.A. 2021-05-28 /pmc/articles/PMC8193062/ /pubmed/34122014 http://dx.doi.org/10.3389/fncel.2021.671549 Text en Copyright © 2021 Puppo, Sadegh, Trujillo, Thunemann, Campbell, Vandenberghe, Shan, Akkouh, Miller, Bloodgood, Silva, Dale, Einevoll, Djurovic, Andreassen, Muotri and Devor. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Puppo, Francesca
Sadegh, Sanaz
Trujillo, Cleber A.
Thunemann, Martin
Campbell, Evan P.
Vandenberghe, Matthieu
Shan, Xiwei
Akkouh, Ibrahim A.
Miller, Evan W.
Bloodgood, Brenda L.
Silva, Gabriel A.
Dale, Anders M.
Einevoll, Gaute T.
Djurovic, Srdjan
Andreassen, Ole A.
Muotri, Alysson R.
Devor, Anna
All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors
title All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors
title_full All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors
title_fullStr All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors
title_full_unstemmed All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors
title_short All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors
title_sort all-optical electrophysiology in hipsc-derived neurons with synthetic voltage sensors
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193062/
https://www.ncbi.nlm.nih.gov/pubmed/34122014
http://dx.doi.org/10.3389/fncel.2021.671549
work_keys_str_mv AT puppofrancesca allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT sadeghsanaz allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT trujilloclebera allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT thunemannmartin allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT campbellevanp allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT vandenberghematthieu allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT shanxiwei allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT akkouhibrahima allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT millerevanw allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT bloodgoodbrendal allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT silvagabriela allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT daleandersm allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT einevollgautet allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT djurovicsrdjan allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT andreassenolea allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT muotrialyssonr allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors
AT devoranna allopticalelectrophysiologyinhipscderivedneuronswithsyntheticvoltagesensors