Crude extracts from Allium cepa skin and Sorghum bicolor seed can provide as non-toxic and eco-friendly cytoplasmic stains

INTRODUCTION: Staining is an important histological process; however, the use of non-toxic and environmentally friendly products is generally required. We explored the staining quality of two natural plants, Allium cepa skin and Sorghum bicolor seed extract on the cytoplasm. MATERIALS AND METHODS: D...

Descripción completa

Detalles Bibliográficos
Autores principales: Krampah, Cecilia, Nyanzu, Franklin, Quaye, Abraham, Adu, Patrick, Asiamah, Emmanuel Akomanin, Aboagye, Benjamin, Simpong, David Larbi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193152/
https://www.ncbi.nlm.nih.gov/pubmed/34150969
http://dx.doi.org/10.1016/j.plabm.2021.e00239
_version_ 1783706195940343808
author Krampah, Cecilia
Nyanzu, Franklin
Quaye, Abraham
Adu, Patrick
Asiamah, Emmanuel Akomanin
Aboagye, Benjamin
Simpong, David Larbi
author_facet Krampah, Cecilia
Nyanzu, Franklin
Quaye, Abraham
Adu, Patrick
Asiamah, Emmanuel Akomanin
Aboagye, Benjamin
Simpong, David Larbi
author_sort Krampah, Cecilia
collection PubMed
description INTRODUCTION: Staining is an important histological process; however, the use of non-toxic and environmentally friendly products is generally required. We explored the staining quality of two natural plants, Allium cepa skin and Sorghum bicolor seed extract on the cytoplasm. MATERIALS AND METHODS: Distilled water at 37 °C and 1% acid-ethanol were respectively used to extract the dyes from Allium cepa skin and Sorghum bicolor seed. RESULT: The application of these two dyes on rodent tissue showed an excellent cytoplasmic histomorphology. CONCLUSION: Allium cepa skin and Sorghum bicolor seed extracts are good cytoplasmic dyes when used as counterstain for haematoxylin.
format Online
Article
Text
id pubmed-8193152
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-81931522021-06-17 Crude extracts from Allium cepa skin and Sorghum bicolor seed can provide as non-toxic and eco-friendly cytoplasmic stains Krampah, Cecilia Nyanzu, Franklin Quaye, Abraham Adu, Patrick Asiamah, Emmanuel Akomanin Aboagye, Benjamin Simpong, David Larbi Pract Lab Med Article INTRODUCTION: Staining is an important histological process; however, the use of non-toxic and environmentally friendly products is generally required. We explored the staining quality of two natural plants, Allium cepa skin and Sorghum bicolor seed extract on the cytoplasm. MATERIALS AND METHODS: Distilled water at 37 °C and 1% acid-ethanol were respectively used to extract the dyes from Allium cepa skin and Sorghum bicolor seed. RESULT: The application of these two dyes on rodent tissue showed an excellent cytoplasmic histomorphology. CONCLUSION: Allium cepa skin and Sorghum bicolor seed extracts are good cytoplasmic dyes when used as counterstain for haematoxylin. Elsevier 2021-06-05 /pmc/articles/PMC8193152/ /pubmed/34150969 http://dx.doi.org/10.1016/j.plabm.2021.e00239 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krampah, Cecilia
Nyanzu, Franklin
Quaye, Abraham
Adu, Patrick
Asiamah, Emmanuel Akomanin
Aboagye, Benjamin
Simpong, David Larbi
Crude extracts from Allium cepa skin and Sorghum bicolor seed can provide as non-toxic and eco-friendly cytoplasmic stains
title Crude extracts from Allium cepa skin and Sorghum bicolor seed can provide as non-toxic and eco-friendly cytoplasmic stains
title_full Crude extracts from Allium cepa skin and Sorghum bicolor seed can provide as non-toxic and eco-friendly cytoplasmic stains
title_fullStr Crude extracts from Allium cepa skin and Sorghum bicolor seed can provide as non-toxic and eco-friendly cytoplasmic stains
title_full_unstemmed Crude extracts from Allium cepa skin and Sorghum bicolor seed can provide as non-toxic and eco-friendly cytoplasmic stains
title_short Crude extracts from Allium cepa skin and Sorghum bicolor seed can provide as non-toxic and eco-friendly cytoplasmic stains
title_sort crude extracts from allium cepa skin and sorghum bicolor seed can provide as non-toxic and eco-friendly cytoplasmic stains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193152/
https://www.ncbi.nlm.nih.gov/pubmed/34150969
http://dx.doi.org/10.1016/j.plabm.2021.e00239
work_keys_str_mv AT krampahcecilia crudeextractsfromalliumcepaskinandsorghumbicolorseedcanprovideasnontoxicandecofriendlycytoplasmicstains
AT nyanzufranklin crudeextractsfromalliumcepaskinandsorghumbicolorseedcanprovideasnontoxicandecofriendlycytoplasmicstains
AT quayeabraham crudeextractsfromalliumcepaskinandsorghumbicolorseedcanprovideasnontoxicandecofriendlycytoplasmicstains
AT adupatrick crudeextractsfromalliumcepaskinandsorghumbicolorseedcanprovideasnontoxicandecofriendlycytoplasmicstains
AT asiamahemmanuelakomanin crudeextractsfromalliumcepaskinandsorghumbicolorseedcanprovideasnontoxicandecofriendlycytoplasmicstains
AT aboagyebenjamin crudeextractsfromalliumcepaskinandsorghumbicolorseedcanprovideasnontoxicandecofriendlycytoplasmicstains
AT simpongdavidlarbi crudeextractsfromalliumcepaskinandsorghumbicolorseedcanprovideasnontoxicandecofriendlycytoplasmicstains

Ejemplares similares