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Decreased Exosomal Acetylcholinesterase Activity in the Plasma of Patients With Parkinson’s Disease

Exosomes, which are small extracellular vesicles produced from various cell types, contain a variety of molecular constituents, such as proteins, lipids, and RNA. Recently, exosomal biomarkers have been investigated to probe the understanding and diagnosis of neurodegenerative disorders. Previous re...

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Autores principales: Shim, Kyu Hwan, Go, Han Gyeol, Bae, Heewon, Jeong, Da-Eun, Kim, Danyeong, Youn, Young Chul, Kim, SangYun, An, Seong Soo A., Kang, Min Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193230/
https://www.ncbi.nlm.nih.gov/pubmed/34122043
http://dx.doi.org/10.3389/fnagi.2021.665400
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author Shim, Kyu Hwan
Go, Han Gyeol
Bae, Heewon
Jeong, Da-Eun
Kim, Danyeong
Youn, Young Chul
Kim, SangYun
An, Seong Soo A.
Kang, Min Ju
author_facet Shim, Kyu Hwan
Go, Han Gyeol
Bae, Heewon
Jeong, Da-Eun
Kim, Danyeong
Youn, Young Chul
Kim, SangYun
An, Seong Soo A.
Kang, Min Ju
author_sort Shim, Kyu Hwan
collection PubMed
description Exosomes, which are small extracellular vesicles produced from various cell types, contain a variety of molecular constituents, such as proteins, lipids, and RNA. Recently, exosomal biomarkers have been investigated to probe the understanding and diagnosis of neurodegenerative disorders. Previous reports have demonstrated increased exosomal α-synuclein (α-syn) in patients with Parkinson’s disease (PD) in comparison to healthy controls (HC). Interestingly, the cholinergic loss was revealed in the central and peripheral nervous systems in histopathology and molecular neuroimaging. Thereby, we simultaneously examined acetylcholinesterase (AChE) with α-syn as exosomal markers. Exosomes were isolated from the plasma of 34 FP-CIT PET proven patients with PD and 29 HC. Exosomal α-syn and AChE activity were quantified andthe relationship with clinical parameters was analyzed. Remarkably, exosomal AChE activity was significantly decreased in PD compared to HC (P = 0.002). Moreover, exosomal AChE activity in PD revealed a strong negative correlation with disease severity, including H&Y (P = 0.007) and UPDRS part III (P = 0.047) scores. By contrast, no significant difference in exosomal α-syn concentration was observed between groups. These results support the occurrence of cholinergic dysfunction in PD, and they could be implicated with disease progression, especially motor deficits. Exosomal AChE activity with advanced exosome isolation techniques may be a reliable biomarker for the early diagnosis and prognosis of PD.
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spelling pubmed-81932302021-06-12 Decreased Exosomal Acetylcholinesterase Activity in the Plasma of Patients With Parkinson’s Disease Shim, Kyu Hwan Go, Han Gyeol Bae, Heewon Jeong, Da-Eun Kim, Danyeong Youn, Young Chul Kim, SangYun An, Seong Soo A. Kang, Min Ju Front Aging Neurosci Neuroscience Exosomes, which are small extracellular vesicles produced from various cell types, contain a variety of molecular constituents, such as proteins, lipids, and RNA. Recently, exosomal biomarkers have been investigated to probe the understanding and diagnosis of neurodegenerative disorders. Previous reports have demonstrated increased exosomal α-synuclein (α-syn) in patients with Parkinson’s disease (PD) in comparison to healthy controls (HC). Interestingly, the cholinergic loss was revealed in the central and peripheral nervous systems in histopathology and molecular neuroimaging. Thereby, we simultaneously examined acetylcholinesterase (AChE) with α-syn as exosomal markers. Exosomes were isolated from the plasma of 34 FP-CIT PET proven patients with PD and 29 HC. Exosomal α-syn and AChE activity were quantified andthe relationship with clinical parameters was analyzed. Remarkably, exosomal AChE activity was significantly decreased in PD compared to HC (P = 0.002). Moreover, exosomal AChE activity in PD revealed a strong negative correlation with disease severity, including H&Y (P = 0.007) and UPDRS part III (P = 0.047) scores. By contrast, no significant difference in exosomal α-syn concentration was observed between groups. These results support the occurrence of cholinergic dysfunction in PD, and they could be implicated with disease progression, especially motor deficits. Exosomal AChE activity with advanced exosome isolation techniques may be a reliable biomarker for the early diagnosis and prognosis of PD. Frontiers Media S.A. 2021-05-28 /pmc/articles/PMC8193230/ /pubmed/34122043 http://dx.doi.org/10.3389/fnagi.2021.665400 Text en Copyright © 2021 Shim, Go, Bae, Jeong, Kim, Youn, Kim, An and Kang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Shim, Kyu Hwan
Go, Han Gyeol
Bae, Heewon
Jeong, Da-Eun
Kim, Danyeong
Youn, Young Chul
Kim, SangYun
An, Seong Soo A.
Kang, Min Ju
Decreased Exosomal Acetylcholinesterase Activity in the Plasma of Patients With Parkinson’s Disease
title Decreased Exosomal Acetylcholinesterase Activity in the Plasma of Patients With Parkinson’s Disease
title_full Decreased Exosomal Acetylcholinesterase Activity in the Plasma of Patients With Parkinson’s Disease
title_fullStr Decreased Exosomal Acetylcholinesterase Activity in the Plasma of Patients With Parkinson’s Disease
title_full_unstemmed Decreased Exosomal Acetylcholinesterase Activity in the Plasma of Patients With Parkinson’s Disease
title_short Decreased Exosomal Acetylcholinesterase Activity in the Plasma of Patients With Parkinson’s Disease
title_sort decreased exosomal acetylcholinesterase activity in the plasma of patients with parkinson’s disease
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193230/
https://www.ncbi.nlm.nih.gov/pubmed/34122043
http://dx.doi.org/10.3389/fnagi.2021.665400
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