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Engineering Escherichia coli for renewable benzyl alcohol production
Benzyl alcohol is an aromatic hydrocarbon used as a solvent and an intermediate chemical in the pharmaceutical, cosmetics, and flavor/fragrance industries. The de novo biosynthesis of benzyl alcohol directly from renewable glucose was herein explored using a non-natural pathway engineered in Escheri...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193235/ https://www.ncbi.nlm.nih.gov/pubmed/34150507 http://dx.doi.org/10.1016/j.meteno.2015.06.002 |
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author | Pugh, Shawn McKenna, Rebekah Halloum, Ibrahim Nielsen, David R. |
author_facet | Pugh, Shawn McKenna, Rebekah Halloum, Ibrahim Nielsen, David R. |
author_sort | Pugh, Shawn |
collection | PubMed |
description | Benzyl alcohol is an aromatic hydrocarbon used as a solvent and an intermediate chemical in the pharmaceutical, cosmetics, and flavor/fragrance industries. The de novo biosynthesis of benzyl alcohol directly from renewable glucose was herein explored using a non-natural pathway engineered in Escherichia coli. Benzaldehyde was first produced from endogenous phenylpyruvate via three heterologous steps, including hydroxymandelate synthase (encoded by hmaS) from Amycolatopsis orientalis, followed by (S)-mandelate dehydrogenase (encoded by mdlB) and phenylglyoxylate decarboxylase (encoded by mdlC) from Pseudomonas putida ATCC 12633. The subsequent rapid and efficient reduction of benzaldehyde to benzyl alcohol occurred by the combined activity and native regulation of multiple endogenous alcohol dehydrogenases and/or aldo-keto reductases. Through systematic deletion of competing aromatic amino acid biosynthesis pathways to promote endogenous phenylpyruvate availability, final benzyl alcohol titers as high as 114±1 mg/L were realized, representing a yield of 7.6±0.1 mg/g on glucose and a ~5-fold improvement over initial strains. |
format | Online Article Text |
id | pubmed-8193235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-81932352021-06-17 Engineering Escherichia coli for renewable benzyl alcohol production Pugh, Shawn McKenna, Rebekah Halloum, Ibrahim Nielsen, David R. Metab Eng Commun Article Benzyl alcohol is an aromatic hydrocarbon used as a solvent and an intermediate chemical in the pharmaceutical, cosmetics, and flavor/fragrance industries. The de novo biosynthesis of benzyl alcohol directly from renewable glucose was herein explored using a non-natural pathway engineered in Escherichia coli. Benzaldehyde was first produced from endogenous phenylpyruvate via three heterologous steps, including hydroxymandelate synthase (encoded by hmaS) from Amycolatopsis orientalis, followed by (S)-mandelate dehydrogenase (encoded by mdlB) and phenylglyoxylate decarboxylase (encoded by mdlC) from Pseudomonas putida ATCC 12633. The subsequent rapid and efficient reduction of benzaldehyde to benzyl alcohol occurred by the combined activity and native regulation of multiple endogenous alcohol dehydrogenases and/or aldo-keto reductases. Through systematic deletion of competing aromatic amino acid biosynthesis pathways to promote endogenous phenylpyruvate availability, final benzyl alcohol titers as high as 114±1 mg/L were realized, representing a yield of 7.6±0.1 mg/g on glucose and a ~5-fold improvement over initial strains. Elsevier 2015-06-19 /pmc/articles/PMC8193235/ /pubmed/34150507 http://dx.doi.org/10.1016/j.meteno.2015.06.002 Text en © 2015 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Pugh, Shawn McKenna, Rebekah Halloum, Ibrahim Nielsen, David R. Engineering Escherichia coli for renewable benzyl alcohol production |
title | Engineering Escherichia coli for renewable benzyl alcohol production |
title_full | Engineering Escherichia coli for renewable benzyl alcohol production |
title_fullStr | Engineering Escherichia coli for renewable benzyl alcohol production |
title_full_unstemmed | Engineering Escherichia coli for renewable benzyl alcohol production |
title_short | Engineering Escherichia coli for renewable benzyl alcohol production |
title_sort | engineering escherichia coli for renewable benzyl alcohol production |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193235/ https://www.ncbi.nlm.nih.gov/pubmed/34150507 http://dx.doi.org/10.1016/j.meteno.2015.06.002 |
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