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Refactoring of a synthetic raspberry ketone pathway with EcoFlex

BACKGROUND:  A key focus of synthetic biology is to develop microbial or cell-free based biobased routes to value-added chemicals such as fragrances. Originally, we developed the EcoFlex system, a Golden Gate toolkit, to study genes/pathways flexibly using Escherichia coli heterologous expression. I...

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Detalles Bibliográficos
Autores principales: Moore, Simon J., Hleba, Yonek B., Bischoff, Sarah, Bell, David, Polizzi, Karen M., Freemont, Paul S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193874/
https://www.ncbi.nlm.nih.gov/pubmed/34112158
http://dx.doi.org/10.1186/s12934-021-01604-4