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Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs

ABSTRACT: BACKGROUND: Mycoplasma suis (M. suis) belongs to the group of haemotrophic mycoplasmas and is known as the causative agent of infectious anaemia in pigs. In the last few years valuable insights into the mechanism of adhesion and invasion, shedding patterns and cell tropism of M. suis were...

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Autores principales: Stadler, Julia, Ade, Julia, Hermanns, Walter, Ritzmann, Mathias, Wentzel, Sarah, Hoelzle, Katharina, Hoelzle, Ludwig E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193878/
https://www.ncbi.nlm.nih.gov/pubmed/34112157
http://dx.doi.org/10.1186/s12917-021-02919-5
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author Stadler, Julia
Ade, Julia
Hermanns, Walter
Ritzmann, Mathias
Wentzel, Sarah
Hoelzle, Katharina
Hoelzle, Ludwig E.
author_facet Stadler, Julia
Ade, Julia
Hermanns, Walter
Ritzmann, Mathias
Wentzel, Sarah
Hoelzle, Katharina
Hoelzle, Ludwig E.
author_sort Stadler, Julia
collection PubMed
description ABSTRACT: BACKGROUND: Mycoplasma suis (M. suis) belongs to the group of haemotrophic mycoplasmas and is known as the causative agent of infectious anaemia in pigs. In the last few years valuable insights into the mechanism of adhesion and invasion, shedding patterns and cell tropism of M. suis were gained by the use of new molecular techniques. However, details on M. suis induced lesions as well as the distribution of M. suis in different organs are still lacking. Therefore, seven splenectomised pigs were experimentally infected and clinical and laboratory investigations as well as a detailed histopathological examination were performed. Detection and quantification of M. suis DNA in blood and various tissue samples was done using a quantitative real-time PCR. RESULTS: During the course of experimental infection, periodically occurring signs of infectious anaemia of pigs including severe icteroanaemia, fever, apathy and anorexia were observed. In addition, dermatological manifestations such as haemorrhagic diathesis presenting as petechiae occurred. The most important haematological alterations were normochromic, normocytic anaemia, hypoglycaemia as well as increased bilirubin and urea concentrations. Necropsy revealed predominant evidence of haemolysis with consecutive anaemia, as well as disseminated intravascular coagulation. M. suis was found in all investigated tissues with the highest copy numbers found in the kidneys. In Giemsa stained sections M. suis was only detected red blood cell (RBC)-associated. CONCLUSION: In the present study, no RBC independent sequestration of M. suis was detected in organs of experimentally infected pigs. Pathological findings are most likely resulting from haemolysis, consecutive anaemia as well as from disseminated intravascular coagulation and subsequent organ impairments.
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spelling pubmed-81938782021-06-15 Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs Stadler, Julia Ade, Julia Hermanns, Walter Ritzmann, Mathias Wentzel, Sarah Hoelzle, Katharina Hoelzle, Ludwig E. BMC Vet Res Research ABSTRACT: BACKGROUND: Mycoplasma suis (M. suis) belongs to the group of haemotrophic mycoplasmas and is known as the causative agent of infectious anaemia in pigs. In the last few years valuable insights into the mechanism of adhesion and invasion, shedding patterns and cell tropism of M. suis were gained by the use of new molecular techniques. However, details on M. suis induced lesions as well as the distribution of M. suis in different organs are still lacking. Therefore, seven splenectomised pigs were experimentally infected and clinical and laboratory investigations as well as a detailed histopathological examination were performed. Detection and quantification of M. suis DNA in blood and various tissue samples was done using a quantitative real-time PCR. RESULTS: During the course of experimental infection, periodically occurring signs of infectious anaemia of pigs including severe icteroanaemia, fever, apathy and anorexia were observed. In addition, dermatological manifestations such as haemorrhagic diathesis presenting as petechiae occurred. The most important haematological alterations were normochromic, normocytic anaemia, hypoglycaemia as well as increased bilirubin and urea concentrations. Necropsy revealed predominant evidence of haemolysis with consecutive anaemia, as well as disseminated intravascular coagulation. M. suis was found in all investigated tissues with the highest copy numbers found in the kidneys. In Giemsa stained sections M. suis was only detected red blood cell (RBC)-associated. CONCLUSION: In the present study, no RBC independent sequestration of M. suis was detected in organs of experimentally infected pigs. Pathological findings are most likely resulting from haemolysis, consecutive anaemia as well as from disseminated intravascular coagulation and subsequent organ impairments. BioMed Central 2021-06-10 /pmc/articles/PMC8193878/ /pubmed/34112157 http://dx.doi.org/10.1186/s12917-021-02919-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Stadler, Julia
Ade, Julia
Hermanns, Walter
Ritzmann, Mathias
Wentzel, Sarah
Hoelzle, Katharina
Hoelzle, Ludwig E.
Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs
title Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs
title_full Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs
title_fullStr Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs
title_full_unstemmed Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs
title_short Clinical, haematological and pathomorphological findings in Mycoplasma suis infected pigs
title_sort clinical, haematological and pathomorphological findings in mycoplasma suis infected pigs
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193878/
https://www.ncbi.nlm.nih.gov/pubmed/34112157
http://dx.doi.org/10.1186/s12917-021-02919-5
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