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Morphological and Rheological Guided Design for the Microencapsulation Process of Lactobacillus paracasei CBA L74 in Calcium Alginate Microspheres

The intestinal microbiota is a real ecosystem composed of several bacterial species and a very huge amount of strains that through their metabolic activities play a crucial role in the development and performance of the immune system and other functions. Microbiota modulation by probiotics establish...

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Autores principales: Di Natale, Concetta, Lagreca, Elena, Panzetta, Valeria, Gallo, Marianna, Passannanti, Francesca, Vitale, Michele, Fusco, Sabato, Vecchione, Raffaele, Nigro, Roberto, Netti, Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193927/
https://www.ncbi.nlm.nih.gov/pubmed/34124020
http://dx.doi.org/10.3389/fbioe.2021.660691
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author Di Natale, Concetta
Lagreca, Elena
Panzetta, Valeria
Gallo, Marianna
Passannanti, Francesca
Vitale, Michele
Fusco, Sabato
Vecchione, Raffaele
Nigro, Roberto
Netti, Paolo
author_facet Di Natale, Concetta
Lagreca, Elena
Panzetta, Valeria
Gallo, Marianna
Passannanti, Francesca
Vitale, Michele
Fusco, Sabato
Vecchione, Raffaele
Nigro, Roberto
Netti, Paolo
author_sort Di Natale, Concetta
collection PubMed
description The intestinal microbiota is a real ecosystem composed of several bacterial species and a very huge amount of strains that through their metabolic activities play a crucial role in the development and performance of the immune system and other functions. Microbiota modulation by probiotics establishes a new era into the pharmaceutical and healthcare market. Probiotics play, in fact, an important role in helping and sustaining human health, but in order to produce benefits, their viability must be preserved throughout the production process up to consumption, and in addition, their bioactivity required to be safeguarded while passing through the gastrointestinal tract. In this frame, encouraging results come from encapsulation strategies that have proven to be very promising in protecting bacteria and their viability. However, specific effort has to be dedicated to the design optimization of the encapsulation process and, in particular, to the processing parameters that affect capsules microstructure. Herein, focusing on calcium alginate microspheres, after a preliminary selection of their processing conditions based on size distribution, we implemented a micro-rheological analysis, by using the multiple-particle tracking technique, to correlate the inner microstructure to the selected process conditions and to the viability of the Lactobacillus paracasei CBA L74. It was assessed that the explored levels of cross-linking, although changing the microorganism constriction, did not affect its viability. The obtained results confirm how this technology is a promising and a valid strategy to protect the microorganism viability and ensure its stability during the production process.
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spelling pubmed-81939272021-06-12 Morphological and Rheological Guided Design for the Microencapsulation Process of Lactobacillus paracasei CBA L74 in Calcium Alginate Microspheres Di Natale, Concetta Lagreca, Elena Panzetta, Valeria Gallo, Marianna Passannanti, Francesca Vitale, Michele Fusco, Sabato Vecchione, Raffaele Nigro, Roberto Netti, Paolo Front Bioeng Biotechnol Bioengineering and Biotechnology The intestinal microbiota is a real ecosystem composed of several bacterial species and a very huge amount of strains that through their metabolic activities play a crucial role in the development and performance of the immune system and other functions. Microbiota modulation by probiotics establishes a new era into the pharmaceutical and healthcare market. Probiotics play, in fact, an important role in helping and sustaining human health, but in order to produce benefits, their viability must be preserved throughout the production process up to consumption, and in addition, their bioactivity required to be safeguarded while passing through the gastrointestinal tract. In this frame, encouraging results come from encapsulation strategies that have proven to be very promising in protecting bacteria and their viability. However, specific effort has to be dedicated to the design optimization of the encapsulation process and, in particular, to the processing parameters that affect capsules microstructure. Herein, focusing on calcium alginate microspheres, after a preliminary selection of their processing conditions based on size distribution, we implemented a micro-rheological analysis, by using the multiple-particle tracking technique, to correlate the inner microstructure to the selected process conditions and to the viability of the Lactobacillus paracasei CBA L74. It was assessed that the explored levels of cross-linking, although changing the microorganism constriction, did not affect its viability. The obtained results confirm how this technology is a promising and a valid strategy to protect the microorganism viability and ensure its stability during the production process. Frontiers Media S.A. 2021-05-28 /pmc/articles/PMC8193927/ /pubmed/34124020 http://dx.doi.org/10.3389/fbioe.2021.660691 Text en Copyright © 2021 Di Natale, Lagreca, Panzetta, Gallo, Passannanti, Vitale, Fusco, Vecchione, Nigro and Netti. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Di Natale, Concetta
Lagreca, Elena
Panzetta, Valeria
Gallo, Marianna
Passannanti, Francesca
Vitale, Michele
Fusco, Sabato
Vecchione, Raffaele
Nigro, Roberto
Netti, Paolo
Morphological and Rheological Guided Design for the Microencapsulation Process of Lactobacillus paracasei CBA L74 in Calcium Alginate Microspheres
title Morphological and Rheological Guided Design for the Microencapsulation Process of Lactobacillus paracasei CBA L74 in Calcium Alginate Microspheres
title_full Morphological and Rheological Guided Design for the Microencapsulation Process of Lactobacillus paracasei CBA L74 in Calcium Alginate Microspheres
title_fullStr Morphological and Rheological Guided Design for the Microencapsulation Process of Lactobacillus paracasei CBA L74 in Calcium Alginate Microspheres
title_full_unstemmed Morphological and Rheological Guided Design for the Microencapsulation Process of Lactobacillus paracasei CBA L74 in Calcium Alginate Microspheres
title_short Morphological and Rheological Guided Design for the Microencapsulation Process of Lactobacillus paracasei CBA L74 in Calcium Alginate Microspheres
title_sort morphological and rheological guided design for the microencapsulation process of lactobacillus paracasei cba l74 in calcium alginate microspheres
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193927/
https://www.ncbi.nlm.nih.gov/pubmed/34124020
http://dx.doi.org/10.3389/fbioe.2021.660691
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