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Conserved RNA Binding Activity of Phosphatidyl Inositol 5-Phosphate 4-Kinase (PIP4K2A)

Plasmodium falciparum is a causative agent for malaria and has a complex life cycle in human and mosquito hosts. During its life cycle, the malarial parasite Plasmodium goes through different asexual and sexual stages, in humans and mosquitoes. Expression of stage-specific proteins is important for...

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Autores principales: Behari, Jatin, Borkar, Pranita, Vindu, Arya, Dandewad, Vishal, Upadrasta, Sindhuri, Shanmugam, Dhanasekaran, Seshadri, Vasudevan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194828/
https://www.ncbi.nlm.nih.gov/pubmed/34124142
http://dx.doi.org/10.3389/fmolb.2021.631281
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author Behari, Jatin
Borkar, Pranita
Vindu, Arya
Dandewad, Vishal
Upadrasta, Sindhuri
Shanmugam, Dhanasekaran
Seshadri, Vasudevan
author_facet Behari, Jatin
Borkar, Pranita
Vindu, Arya
Dandewad, Vishal
Upadrasta, Sindhuri
Shanmugam, Dhanasekaran
Seshadri, Vasudevan
author_sort Behari, Jatin
collection PubMed
description Plasmodium falciparum is a causative agent for malaria and has a complex life cycle in human and mosquito hosts. During its life cycle, the malarial parasite Plasmodium goes through different asexual and sexual stages, in humans and mosquitoes. Expression of stage-specific proteins is important for successful completion of its life cycle and requires tight gene regulation. In the case of Plasmodium, due to relative paucity of the transcription factors, it is postulated that posttranscriptional regulation plays an important role in stage-specific gene expression. Translation repression of specific set of mRNA has been reported in gametocyte stages of the parasite. A conserved element present in the 3′UTR of some of these transcripts was identified. Phosphatidylinositol 5-phosphate 4-kinase (PIP4K2A) was identified as the protein that associates with these RNA. We now show that the RNA binding activity of PIP4K2A is independent of its kinase activity. We also observe that PIP4K2A is imported into the parasite from the host on Plasmodium berghei and Toxoplasma gondii. The RNA binding activity of PIP4K2A seems to be conserved across species from Drosophila and C. elegans to humans, suggesting that the RNA binding activity of PIP4K may be important, and there may be host transcripts that may be regulated by PIP4K2A. These results identify a novel RNA binding role for PIP4K2A that may not only play a role in Plasmodium propagation but may also function in regulating gene expression in multicellular organisms.
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spelling pubmed-81948282021-06-12 Conserved RNA Binding Activity of Phosphatidyl Inositol 5-Phosphate 4-Kinase (PIP4K2A) Behari, Jatin Borkar, Pranita Vindu, Arya Dandewad, Vishal Upadrasta, Sindhuri Shanmugam, Dhanasekaran Seshadri, Vasudevan Front Mol Biosci Molecular Biosciences Plasmodium falciparum is a causative agent for malaria and has a complex life cycle in human and mosquito hosts. During its life cycle, the malarial parasite Plasmodium goes through different asexual and sexual stages, in humans and mosquitoes. Expression of stage-specific proteins is important for successful completion of its life cycle and requires tight gene regulation. In the case of Plasmodium, due to relative paucity of the transcription factors, it is postulated that posttranscriptional regulation plays an important role in stage-specific gene expression. Translation repression of specific set of mRNA has been reported in gametocyte stages of the parasite. A conserved element present in the 3′UTR of some of these transcripts was identified. Phosphatidylinositol 5-phosphate 4-kinase (PIP4K2A) was identified as the protein that associates with these RNA. We now show that the RNA binding activity of PIP4K2A is independent of its kinase activity. We also observe that PIP4K2A is imported into the parasite from the host on Plasmodium berghei and Toxoplasma gondii. The RNA binding activity of PIP4K2A seems to be conserved across species from Drosophila and C. elegans to humans, suggesting that the RNA binding activity of PIP4K may be important, and there may be host transcripts that may be regulated by PIP4K2A. These results identify a novel RNA binding role for PIP4K2A that may not only play a role in Plasmodium propagation but may also function in regulating gene expression in multicellular organisms. Frontiers Media S.A. 2021-05-28 /pmc/articles/PMC8194828/ /pubmed/34124142 http://dx.doi.org/10.3389/fmolb.2021.631281 Text en Copyright © 2021 Behari, Borkar, Vindu, Dandewad, Upadrasta, Shanmugam and Seshadri. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Behari, Jatin
Borkar, Pranita
Vindu, Arya
Dandewad, Vishal
Upadrasta, Sindhuri
Shanmugam, Dhanasekaran
Seshadri, Vasudevan
Conserved RNA Binding Activity of Phosphatidyl Inositol 5-Phosphate 4-Kinase (PIP4K2A)
title Conserved RNA Binding Activity of Phosphatidyl Inositol 5-Phosphate 4-Kinase (PIP4K2A)
title_full Conserved RNA Binding Activity of Phosphatidyl Inositol 5-Phosphate 4-Kinase (PIP4K2A)
title_fullStr Conserved RNA Binding Activity of Phosphatidyl Inositol 5-Phosphate 4-Kinase (PIP4K2A)
title_full_unstemmed Conserved RNA Binding Activity of Phosphatidyl Inositol 5-Phosphate 4-Kinase (PIP4K2A)
title_short Conserved RNA Binding Activity of Phosphatidyl Inositol 5-Phosphate 4-Kinase (PIP4K2A)
title_sort conserved rna binding activity of phosphatidyl inositol 5-phosphate 4-kinase (pip4k2a)
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194828/
https://www.ncbi.nlm.nih.gov/pubmed/34124142
http://dx.doi.org/10.3389/fmolb.2021.631281
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