Cargando…
Effects of germinating temperature and time on metabolite profiles of sunflower (Helianthus annuus L.) seed
Sprouts with higher levels of nutrients and lower content of antinutritional substances have been gained a growing interest in the influence on the human's health. The study of the influence of germination temperature and time on the metabolite profiles of sunflower seed was studied by a metabo...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194965/ https://www.ncbi.nlm.nih.gov/pubmed/34136149 http://dx.doi.org/10.1002/fsn3.1983 |
_version_ | 1783706454464659456 |
---|---|
author | Guo, Shuangshuang Klinkesorn, Utai Lorjaroenphon, Yaowapa Ge, Yan Na Jom, Kriskamol |
author_facet | Guo, Shuangshuang Klinkesorn, Utai Lorjaroenphon, Yaowapa Ge, Yan Na Jom, Kriskamol |
author_sort | Guo, Shuangshuang |
collection | PubMed |
description | Sprouts with higher levels of nutrients and lower content of antinutritional substances have been gained a growing interest in the influence on the human's health. The study of the influence of germination temperature and time on the metabolite profiles of sunflower seed was studied by a metabolomics approach based on gas chromatography–flame ionization detection (GC‐FID). Samples were extracted and fractionated covering a wide range of lipophilic and hydrophilic spectra. A total of 90 metabolites were identified by comparison with reference standards. Principal component analysis (PCA) revealed distinct dynamic changes in metabolites with the germinating time. Heatmap and agglomerative hierarchical clustering analysis revealed the differences and similarities among the samples. The germinating sunflower seeds clustered into three major groups. For instance, group I with a high content of sterols, monosaccharide, and amino acids, indicating the germination process, resulted in an increase in amino acids and monosaccharide. Group II had a high content of FAME and FFA. Relative targeted quantification of metabolites visually depicted by heatmap showed decreases in fatty acid methyl ester (FAME) and free fatty acid (FFA), and increases in amino acids, α‐tocopherol, sterols, and γ‐aminobutyric acid (GABA) during germination. Sunflower seeds germinated at 25°C were better for the accumulation of α‐tocopherol, stigmasterol, leucine, proline, methionine, glutamine, and GABA compared with those at 35°C. These results help to better understand how germination conditions change the nutritional quality of germinated sunflower seeds from a metabolite profile view, allowing for the rational screening and usage of germinated sunflower seeds in the food industry. |
format | Online Article Text |
id | pubmed-8194965 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81949652021-06-15 Effects of germinating temperature and time on metabolite profiles of sunflower (Helianthus annuus L.) seed Guo, Shuangshuang Klinkesorn, Utai Lorjaroenphon, Yaowapa Ge, Yan Na Jom, Kriskamol Food Sci Nutr Original Research Sprouts with higher levels of nutrients and lower content of antinutritional substances have been gained a growing interest in the influence on the human's health. The study of the influence of germination temperature and time on the metabolite profiles of sunflower seed was studied by a metabolomics approach based on gas chromatography–flame ionization detection (GC‐FID). Samples were extracted and fractionated covering a wide range of lipophilic and hydrophilic spectra. A total of 90 metabolites were identified by comparison with reference standards. Principal component analysis (PCA) revealed distinct dynamic changes in metabolites with the germinating time. Heatmap and agglomerative hierarchical clustering analysis revealed the differences and similarities among the samples. The germinating sunflower seeds clustered into three major groups. For instance, group I with a high content of sterols, monosaccharide, and amino acids, indicating the germination process, resulted in an increase in amino acids and monosaccharide. Group II had a high content of FAME and FFA. Relative targeted quantification of metabolites visually depicted by heatmap showed decreases in fatty acid methyl ester (FAME) and free fatty acid (FFA), and increases in amino acids, α‐tocopherol, sterols, and γ‐aminobutyric acid (GABA) during germination. Sunflower seeds germinated at 25°C were better for the accumulation of α‐tocopherol, stigmasterol, leucine, proline, methionine, glutamine, and GABA compared with those at 35°C. These results help to better understand how germination conditions change the nutritional quality of germinated sunflower seeds from a metabolite profile view, allowing for the rational screening and usage of germinated sunflower seeds in the food industry. John Wiley and Sons Inc. 2021-05-05 /pmc/articles/PMC8194965/ /pubmed/34136149 http://dx.doi.org/10.1002/fsn3.1983 Text en © 2021 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Guo, Shuangshuang Klinkesorn, Utai Lorjaroenphon, Yaowapa Ge, Yan Na Jom, Kriskamol Effects of germinating temperature and time on metabolite profiles of sunflower (Helianthus annuus L.) seed |
title | Effects of germinating temperature and time on metabolite profiles of sunflower (Helianthus annuus L.) seed |
title_full | Effects of germinating temperature and time on metabolite profiles of sunflower (Helianthus annuus L.) seed |
title_fullStr | Effects of germinating temperature and time on metabolite profiles of sunflower (Helianthus annuus L.) seed |
title_full_unstemmed | Effects of germinating temperature and time on metabolite profiles of sunflower (Helianthus annuus L.) seed |
title_short | Effects of germinating temperature and time on metabolite profiles of sunflower (Helianthus annuus L.) seed |
title_sort | effects of germinating temperature and time on metabolite profiles of sunflower (helianthus annuus l.) seed |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194965/ https://www.ncbi.nlm.nih.gov/pubmed/34136149 http://dx.doi.org/10.1002/fsn3.1983 |
work_keys_str_mv | AT guoshuangshuang effectsofgerminatingtemperatureandtimeonmetaboliteprofilesofsunflowerhelianthusannuuslseed AT klinkesornutai effectsofgerminatingtemperatureandtimeonmetaboliteprofilesofsunflowerhelianthusannuuslseed AT lorjaroenphonyaowapa effectsofgerminatingtemperatureandtimeonmetaboliteprofilesofsunflowerhelianthusannuuslseed AT geyan effectsofgerminatingtemperatureandtimeonmetaboliteprofilesofsunflowerhelianthusannuuslseed AT najomkriskamol effectsofgerminatingtemperatureandtimeonmetaboliteprofilesofsunflowerhelianthusannuuslseed |