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Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination
Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by Aedes aegypti mosquitoes. Habitat expansion of Aedes, mainly due to climate change and increasing overlap between urban and wild habitats, places nearly half of the world’s...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195420/ https://www.ncbi.nlm.nih.gov/pubmed/34115766 http://dx.doi.org/10.1371/journal.pntd.0009442 |
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author | Marin-Lopez, Alejandro Jiang, Junjun Wang, Yuchen Cao, Yongguo MacNeil, Tyler Hastings, Andrew K. Fikrig, Erol |
author_facet | Marin-Lopez, Alejandro Jiang, Junjun Wang, Yuchen Cao, Yongguo MacNeil, Tyler Hastings, Andrew K. Fikrig, Erol |
author_sort | Marin-Lopez, Alejandro |
collection | PubMed |
description | Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by Aedes aegypti mosquitoes. Habitat expansion of Aedes, mainly due to climate change and increasing overlap between urban and wild habitats, places nearly half of the world’s population at risk for DENV infection. After a bloodmeal from a DENV-infected host, the virus enters the mosquito midgut. Next, the virus migrates to, and replicates in, other tissues, like salivary glands. Successful viral transmission occurs when the infected mosquito takes another blood meal on a susceptible host and DENV is released from the salivary gland via saliva into the skin. During viral dissemination in the mosquito and transmission to a new mammalian host, DENV interacts with a variety of vector proteins, which are uniquely important during each phase of the viral cycle. Our study focuses on the interaction between DENV particles and protein components in the A. aegypti vector. We performed a mass spectrometry assay where we identified a set of A. aegypti salivary gland proteins which potentially interact with the DENV virion. Using dsRNA to silence gene expression, we analyzed the role of these proteins in viral infectivity. Two of these candidates, a synaptosomal-associated protein (AeSNAP) and a calcium transporter ATPase (ATPase) appear to play a role in viral replication both in vitro and in vivo, observing a ubiquitous expression of these proteins in the mosquito. These findings suggest that AeSNAP plays a protective role during DENV infection of mosquitoes and that ATPase protein is required for DENV during amplification within the vector. |
format | Online Article Text |
id | pubmed-8195420 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-81954202021-06-21 Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination Marin-Lopez, Alejandro Jiang, Junjun Wang, Yuchen Cao, Yongguo MacNeil, Tyler Hastings, Andrew K. Fikrig, Erol PLoS Negl Trop Dis Research Article Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by Aedes aegypti mosquitoes. Habitat expansion of Aedes, mainly due to climate change and increasing overlap between urban and wild habitats, places nearly half of the world’s population at risk for DENV infection. After a bloodmeal from a DENV-infected host, the virus enters the mosquito midgut. Next, the virus migrates to, and replicates in, other tissues, like salivary glands. Successful viral transmission occurs when the infected mosquito takes another blood meal on a susceptible host and DENV is released from the salivary gland via saliva into the skin. During viral dissemination in the mosquito and transmission to a new mammalian host, DENV interacts with a variety of vector proteins, which are uniquely important during each phase of the viral cycle. Our study focuses on the interaction between DENV particles and protein components in the A. aegypti vector. We performed a mass spectrometry assay where we identified a set of A. aegypti salivary gland proteins which potentially interact with the DENV virion. Using dsRNA to silence gene expression, we analyzed the role of these proteins in viral infectivity. Two of these candidates, a synaptosomal-associated protein (AeSNAP) and a calcium transporter ATPase (ATPase) appear to play a role in viral replication both in vitro and in vivo, observing a ubiquitous expression of these proteins in the mosquito. These findings suggest that AeSNAP plays a protective role during DENV infection of mosquitoes and that ATPase protein is required for DENV during amplification within the vector. Public Library of Science 2021-06-11 /pmc/articles/PMC8195420/ /pubmed/34115766 http://dx.doi.org/10.1371/journal.pntd.0009442 Text en © 2021 Marin-Lopez et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Marin-Lopez, Alejandro Jiang, Junjun Wang, Yuchen Cao, Yongguo MacNeil, Tyler Hastings, Andrew K. Fikrig, Erol Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination |
title | Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination |
title_full | Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination |
title_fullStr | Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination |
title_full_unstemmed | Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination |
title_short | Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination |
title_sort | aedes aegypti snap and a calcium transporter atpase influence dengue virus dissemination |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195420/ https://www.ncbi.nlm.nih.gov/pubmed/34115766 http://dx.doi.org/10.1371/journal.pntd.0009442 |
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