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Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination

Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by Aedes aegypti mosquitoes. Habitat expansion of Aedes, mainly due to climate change and increasing overlap between urban and wild habitats, places nearly half of the world’s...

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Autores principales: Marin-Lopez, Alejandro, Jiang, Junjun, Wang, Yuchen, Cao, Yongguo, MacNeil, Tyler, Hastings, Andrew K., Fikrig, Erol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195420/
https://www.ncbi.nlm.nih.gov/pubmed/34115766
http://dx.doi.org/10.1371/journal.pntd.0009442
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author Marin-Lopez, Alejandro
Jiang, Junjun
Wang, Yuchen
Cao, Yongguo
MacNeil, Tyler
Hastings, Andrew K.
Fikrig, Erol
author_facet Marin-Lopez, Alejandro
Jiang, Junjun
Wang, Yuchen
Cao, Yongguo
MacNeil, Tyler
Hastings, Andrew K.
Fikrig, Erol
author_sort Marin-Lopez, Alejandro
collection PubMed
description Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by Aedes aegypti mosquitoes. Habitat expansion of Aedes, mainly due to climate change and increasing overlap between urban and wild habitats, places nearly half of the world’s population at risk for DENV infection. After a bloodmeal from a DENV-infected host, the virus enters the mosquito midgut. Next, the virus migrates to, and replicates in, other tissues, like salivary glands. Successful viral transmission occurs when the infected mosquito takes another blood meal on a susceptible host and DENV is released from the salivary gland via saliva into the skin. During viral dissemination in the mosquito and transmission to a new mammalian host, DENV interacts with a variety of vector proteins, which are uniquely important during each phase of the viral cycle. Our study focuses on the interaction between DENV particles and protein components in the A. aegypti vector. We performed a mass spectrometry assay where we identified a set of A. aegypti salivary gland proteins which potentially interact with the DENV virion. Using dsRNA to silence gene expression, we analyzed the role of these proteins in viral infectivity. Two of these candidates, a synaptosomal-associated protein (AeSNAP) and a calcium transporter ATPase (ATPase) appear to play a role in viral replication both in vitro and in vivo, observing a ubiquitous expression of these proteins in the mosquito. These findings suggest that AeSNAP plays a protective role during DENV infection of mosquitoes and that ATPase protein is required for DENV during amplification within the vector.
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spelling pubmed-81954202021-06-21 Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination Marin-Lopez, Alejandro Jiang, Junjun Wang, Yuchen Cao, Yongguo MacNeil, Tyler Hastings, Andrew K. Fikrig, Erol PLoS Negl Trop Dis Research Article Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by Aedes aegypti mosquitoes. Habitat expansion of Aedes, mainly due to climate change and increasing overlap between urban and wild habitats, places nearly half of the world’s population at risk for DENV infection. After a bloodmeal from a DENV-infected host, the virus enters the mosquito midgut. Next, the virus migrates to, and replicates in, other tissues, like salivary glands. Successful viral transmission occurs when the infected mosquito takes another blood meal on a susceptible host and DENV is released from the salivary gland via saliva into the skin. During viral dissemination in the mosquito and transmission to a new mammalian host, DENV interacts with a variety of vector proteins, which are uniquely important during each phase of the viral cycle. Our study focuses on the interaction between DENV particles and protein components in the A. aegypti vector. We performed a mass spectrometry assay where we identified a set of A. aegypti salivary gland proteins which potentially interact with the DENV virion. Using dsRNA to silence gene expression, we analyzed the role of these proteins in viral infectivity. Two of these candidates, a synaptosomal-associated protein (AeSNAP) and a calcium transporter ATPase (ATPase) appear to play a role in viral replication both in vitro and in vivo, observing a ubiquitous expression of these proteins in the mosquito. These findings suggest that AeSNAP plays a protective role during DENV infection of mosquitoes and that ATPase protein is required for DENV during amplification within the vector. Public Library of Science 2021-06-11 /pmc/articles/PMC8195420/ /pubmed/34115766 http://dx.doi.org/10.1371/journal.pntd.0009442 Text en © 2021 Marin-Lopez et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Marin-Lopez, Alejandro
Jiang, Junjun
Wang, Yuchen
Cao, Yongguo
MacNeil, Tyler
Hastings, Andrew K.
Fikrig, Erol
Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination
title Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination
title_full Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination
title_fullStr Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination
title_full_unstemmed Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination
title_short Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination
title_sort aedes aegypti snap and a calcium transporter atpase influence dengue virus dissemination
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195420/
https://www.ncbi.nlm.nih.gov/pubmed/34115766
http://dx.doi.org/10.1371/journal.pntd.0009442
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