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Essential Role of CFAP53 in Sperm Flagellum Biogenesis

The sperm flagellum is essential for male fertility. Despite vigorous research progress toward understanding the pathogenesis of flagellum-related diseases, much remains unknown about the mechanisms underlying the flagellum biogenesis itself. Here, we show that the cilia and flagella associated prot...

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Autores principales: Wu, Bingbing, Yu, Xiaochen, Liu, Chao, Wang, Lina, Huang, Tao, Lu, Gang, Chen, Zi-Jiang, Li, Wei, Liu, Hongbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195676/
https://www.ncbi.nlm.nih.gov/pubmed/34124066
http://dx.doi.org/10.3389/fcell.2021.676910
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author Wu, Bingbing
Yu, Xiaochen
Liu, Chao
Wang, Lina
Huang, Tao
Lu, Gang
Chen, Zi-Jiang
Li, Wei
Liu, Hongbin
author_facet Wu, Bingbing
Yu, Xiaochen
Liu, Chao
Wang, Lina
Huang, Tao
Lu, Gang
Chen, Zi-Jiang
Li, Wei
Liu, Hongbin
author_sort Wu, Bingbing
collection PubMed
description The sperm flagellum is essential for male fertility. Despite vigorous research progress toward understanding the pathogenesis of flagellum-related diseases, much remains unknown about the mechanisms underlying the flagellum biogenesis itself. Here, we show that the cilia and flagella associated protein 53 (Cfap53) gene is predominantly expressed in testes, and it is essential for sperm flagellum biogenesis. The knockout of this gene resulted in complete infertility in male mice but not in the females. CFAP53 localized to the manchette and sperm tail during spermiogenesis, the knockout of this gene impaired flagellum biogenesis. Furthermore, we identified two manchette and sperm tail-associated proteins that interacted with CFAP53 during spermiogenesis. Together, our results suggest that CFAP53 is an essential protein for sperm flagellum biogenesis, and its mutations might be associated with multiple morphological abnormalities of the flagella (MMAF).
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spelling pubmed-81956762021-06-12 Essential Role of CFAP53 in Sperm Flagellum Biogenesis Wu, Bingbing Yu, Xiaochen Liu, Chao Wang, Lina Huang, Tao Lu, Gang Chen, Zi-Jiang Li, Wei Liu, Hongbin Front Cell Dev Biol Cell and Developmental Biology The sperm flagellum is essential for male fertility. Despite vigorous research progress toward understanding the pathogenesis of flagellum-related diseases, much remains unknown about the mechanisms underlying the flagellum biogenesis itself. Here, we show that the cilia and flagella associated protein 53 (Cfap53) gene is predominantly expressed in testes, and it is essential for sperm flagellum biogenesis. The knockout of this gene resulted in complete infertility in male mice but not in the females. CFAP53 localized to the manchette and sperm tail during spermiogenesis, the knockout of this gene impaired flagellum biogenesis. Furthermore, we identified two manchette and sperm tail-associated proteins that interacted with CFAP53 during spermiogenesis. Together, our results suggest that CFAP53 is an essential protein for sperm flagellum biogenesis, and its mutations might be associated with multiple morphological abnormalities of the flagella (MMAF). Frontiers Media S.A. 2021-05-28 /pmc/articles/PMC8195676/ /pubmed/34124066 http://dx.doi.org/10.3389/fcell.2021.676910 Text en Copyright © 2021 Wu, Yu, Liu, Wang, Huang, Lu, Chen, Li and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Wu, Bingbing
Yu, Xiaochen
Liu, Chao
Wang, Lina
Huang, Tao
Lu, Gang
Chen, Zi-Jiang
Li, Wei
Liu, Hongbin
Essential Role of CFAP53 in Sperm Flagellum Biogenesis
title Essential Role of CFAP53 in Sperm Flagellum Biogenesis
title_full Essential Role of CFAP53 in Sperm Flagellum Biogenesis
title_fullStr Essential Role of CFAP53 in Sperm Flagellum Biogenesis
title_full_unstemmed Essential Role of CFAP53 in Sperm Flagellum Biogenesis
title_short Essential Role of CFAP53 in Sperm Flagellum Biogenesis
title_sort essential role of cfap53 in sperm flagellum biogenesis
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195676/
https://www.ncbi.nlm.nih.gov/pubmed/34124066
http://dx.doi.org/10.3389/fcell.2021.676910
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