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Amplicon-sequencing of raw milk microbiota: impact of DNA extraction and library-PCR

ABSTRACT: The highly complex raw milk matrix challenges the sample preparation for amplicon-sequencing due to low bacterial counts and high amounts of eukaryotic DNA originating from the cow. In this study, we optimized the extraction of bacterial DNA from raw milk for microbiome analysis and evalua...

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Detalles Bibliográficos
Autores principales:	Siebert, Annemarie, Hofmann, Katharina, Staib, Lena, Doll, Etienne V., Scherer, Siegfried, Wenning, Mareike
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2021
Materias:	Methods and Protocols
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195793/ https://www.ncbi.nlm.nih.gov/pubmed/34059942 http://dx.doi.org/10.1007/s00253-021-11353-4

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