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AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division

Argonaute 2 (AGO2) is an indispensable component of the RNA-induced silencing complex, operating at the translational or posttranscriptional level. It is compartmentalized into structures such as GW- and P-bodies, stress granules and adherens junctions as well as the midbody. Here we show using immu...

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Autores principales: Pantazopoulou, Vasiliki I., Delis, Anastasios D., Georgiou, Stella, Pagakis, Stamatis N., Filippa, Vicky, Dragona, Eleni, Kloukina, Ismini, Chatzitheodoridis, Elias, Trebicka, Jonel, Velentzas, Athanassios D., Thiele, Maja, Gagos, Sarantis, Thanos, Dimitris, Tseleni-Balafouta, Sofia, Stravopodis, Dimitrios J., Anastasiadou, Ema
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196063/
https://www.ncbi.nlm.nih.gov/pubmed/34117353
http://dx.doi.org/10.1038/s42003-021-02130-0
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author Pantazopoulou, Vasiliki I.
Delis, Anastasios D.
Georgiou, Stella
Pagakis, Stamatis N.
Filippa, Vicky
Dragona, Eleni
Kloukina, Ismini
Chatzitheodoridis, Elias
Trebicka, Jonel
Velentzas, Athanassios D.
Thiele, Maja
Gagos, Sarantis
Thanos, Dimitris
Tseleni-Balafouta, Sofia
Stravopodis, Dimitrios J.
Anastasiadou, Ema
author_facet Pantazopoulou, Vasiliki I.
Delis, Anastasios D.
Georgiou, Stella
Pagakis, Stamatis N.
Filippa, Vicky
Dragona, Eleni
Kloukina, Ismini
Chatzitheodoridis, Elias
Trebicka, Jonel
Velentzas, Athanassios D.
Thiele, Maja
Gagos, Sarantis
Thanos, Dimitris
Tseleni-Balafouta, Sofia
Stravopodis, Dimitrios J.
Anastasiadou, Ema
author_sort Pantazopoulou, Vasiliki I.
collection PubMed
description Argonaute 2 (AGO2) is an indispensable component of the RNA-induced silencing complex, operating at the translational or posttranscriptional level. It is compartmentalized into structures such as GW- and P-bodies, stress granules and adherens junctions as well as the midbody. Here we show using immunofluorescence, image and bioinformatic analysis and cytogenetics that AGO2 also resides in membrane protrusions such as open- and close-ended tubes. The latter are cytokinetic bridges where AGO2 colocalizes at the midbody arms with cytoskeletal components such as α-Τubulin and Aurora B, and various kinases. AGO2, phosphorylated on serine 387, is located together with Dicer at the midbody ring in a manner dependent on p38 MAPK activity. We further show that AGO2 is stress sensitive and important to ensure the proper chromosome segregation and cytokinetic fidelity. We suggest that AGO2 is part of a regulatory mechanism triggered by cytokinetic stress to generate the appropriate micro-environment for local transcript homeostasis.
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spelling pubmed-81960632021-06-17 AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division Pantazopoulou, Vasiliki I. Delis, Anastasios D. Georgiou, Stella Pagakis, Stamatis N. Filippa, Vicky Dragona, Eleni Kloukina, Ismini Chatzitheodoridis, Elias Trebicka, Jonel Velentzas, Athanassios D. Thiele, Maja Gagos, Sarantis Thanos, Dimitris Tseleni-Balafouta, Sofia Stravopodis, Dimitrios J. Anastasiadou, Ema Commun Biol Article Argonaute 2 (AGO2) is an indispensable component of the RNA-induced silencing complex, operating at the translational or posttranscriptional level. It is compartmentalized into structures such as GW- and P-bodies, stress granules and adherens junctions as well as the midbody. Here we show using immunofluorescence, image and bioinformatic analysis and cytogenetics that AGO2 also resides in membrane protrusions such as open- and close-ended tubes. The latter are cytokinetic bridges where AGO2 colocalizes at the midbody arms with cytoskeletal components such as α-Τubulin and Aurora B, and various kinases. AGO2, phosphorylated on serine 387, is located together with Dicer at the midbody ring in a manner dependent on p38 MAPK activity. We further show that AGO2 is stress sensitive and important to ensure the proper chromosome segregation and cytokinetic fidelity. We suggest that AGO2 is part of a regulatory mechanism triggered by cytokinetic stress to generate the appropriate micro-environment for local transcript homeostasis. Nature Publishing Group UK 2021-06-11 /pmc/articles/PMC8196063/ /pubmed/34117353 http://dx.doi.org/10.1038/s42003-021-02130-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Pantazopoulou, Vasiliki I.
Delis, Anastasios D.
Georgiou, Stella
Pagakis, Stamatis N.
Filippa, Vicky
Dragona, Eleni
Kloukina, Ismini
Chatzitheodoridis, Elias
Trebicka, Jonel
Velentzas, Athanassios D.
Thiele, Maja
Gagos, Sarantis
Thanos, Dimitris
Tseleni-Balafouta, Sofia
Stravopodis, Dimitrios J.
Anastasiadou, Ema
AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division
title AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division
title_full AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division
title_fullStr AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division
title_full_unstemmed AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division
title_short AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division
title_sort ago2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196063/
https://www.ncbi.nlm.nih.gov/pubmed/34117353
http://dx.doi.org/10.1038/s42003-021-02130-0
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