Protocol for determining protein cysteine thiol redox status using western blot analysis

This protocol describes the analysis of protein cysteine redox status. Redox status is crucial in regulating protein activity, stability, and redox signaling cascades. It is determined by conjugation with 1.24 kDa MM(PEG)(24) molecule to each reduced cysteine followed by western blot analysis. This...

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Detalles Bibliográficos
Autores principales: Pant, Bikram Datt, Oh, Sunhee, Mysore, Kirankumar S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196218/
https://www.ncbi.nlm.nih.gov/pubmed/34159320
http://dx.doi.org/10.1016/j.xpro.2021.100566
Descripción
Sumario:This protocol describes the analysis of protein cysteine redox status. Redox status is crucial in regulating protein activity, stability, and redox signaling cascades. It is determined by conjugation with 1.24 kDa MM(PEG)(24) molecule to each reduced cysteine followed by western blot analysis. This protocol is easy to follow, and most of the reagents and instruments required are of common use in any lab. This protocol can be successfully applied to other biological sources. For complete details on the use and execution of this protocol, please refer to Pant et al. (2020).

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