Protocol for determining protein cysteine thiol redox status using western blot analysis

This protocol describes the analysis of protein cysteine redox status. Redox status is crucial in regulating protein activity, stability, and redox signaling cascades. It is determined by conjugation with 1.24 kDa MM(PEG)(24) molecule to each reduced cysteine followed by western blot analysis. This...

Descripción completa

Detalles Bibliográficos
Autores principales: Pant, Bikram Datt, Oh, Sunhee, Mysore, Kirankumar S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196218/
https://www.ncbi.nlm.nih.gov/pubmed/34159320
http://dx.doi.org/10.1016/j.xpro.2021.100566
_version_ 1783706640495673344
author Pant, Bikram Datt
Oh, Sunhee
Mysore, Kirankumar S.
author_facet Pant, Bikram Datt
Oh, Sunhee
Mysore, Kirankumar S.
author_sort Pant, Bikram Datt
collection PubMed
description This protocol describes the analysis of protein cysteine redox status. Redox status is crucial in regulating protein activity, stability, and redox signaling cascades. It is determined by conjugation with 1.24 kDa MM(PEG)(24) molecule to each reduced cysteine followed by western blot analysis. This protocol is easy to follow, and most of the reagents and instruments required are of common use in any lab. This protocol can be successfully applied to other biological sources. For complete details on the use and execution of this protocol, please refer to Pant et al. (2020).
format Online
Article
Text
id pubmed-8196218
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-81962182021-06-21 Protocol for determining protein cysteine thiol redox status using western blot analysis Pant, Bikram Datt Oh, Sunhee Mysore, Kirankumar S. STAR Protoc Protocol This protocol describes the analysis of protein cysteine redox status. Redox status is crucial in regulating protein activity, stability, and redox signaling cascades. It is determined by conjugation with 1.24 kDa MM(PEG)(24) molecule to each reduced cysteine followed by western blot analysis. This protocol is easy to follow, and most of the reagents and instruments required are of common use in any lab. This protocol can be successfully applied to other biological sources. For complete details on the use and execution of this protocol, please refer to Pant et al. (2020). Elsevier 2021-06-09 /pmc/articles/PMC8196218/ /pubmed/34159320 http://dx.doi.org/10.1016/j.xpro.2021.100566 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Pant, Bikram Datt
Oh, Sunhee
Mysore, Kirankumar S.
Protocol for determining protein cysteine thiol redox status using western blot analysis
title Protocol for determining protein cysteine thiol redox status using western blot analysis
title_full Protocol for determining protein cysteine thiol redox status using western blot analysis
title_fullStr Protocol for determining protein cysteine thiol redox status using western blot analysis
title_full_unstemmed Protocol for determining protein cysteine thiol redox status using western blot analysis
title_short Protocol for determining protein cysteine thiol redox status using western blot analysis
title_sort protocol for determining protein cysteine thiol redox status using western blot analysis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196218/
https://www.ncbi.nlm.nih.gov/pubmed/34159320
http://dx.doi.org/10.1016/j.xpro.2021.100566
work_keys_str_mv AT pantbikramdatt protocolfordeterminingproteincysteinethiolredoxstatususingwesternblotanalysis
AT ohsunhee protocolfordeterminingproteincysteinethiolredoxstatususingwesternblotanalysis
AT mysorekirankumars protocolfordeterminingproteincysteinethiolredoxstatususingwesternblotanalysis

Ejemplares similares