Cargando…

Application of Metagenomic Next-Generation Sequencing to Diagnose Pneumocystis jirovecii Pneumonia in Kidney Transplantation Recipients

BACKGROUND: Pneumocystis jirovecii pneumonia (PJP) is one of the common opportunistic infections diagnosed in kidney transplantation recipients. It is difficult to identify early by use of classic tools such as Grocott-Gomori stains and polymerase chain reaction (PCR). Metagenomic next-generation se...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Jia, Yu, Yedong, Lv, Junhao, Yang, Sisi, Wu, Jianyong, Chen, Jianghua, Peng, Wenhan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196548/
https://www.ncbi.nlm.nih.gov/pubmed/34099614
http://dx.doi.org/10.12659/AOT.931059
_version_ 1783706711254630400
author Xu, Jia
Yu, Yedong
Lv, Junhao
Yang, Sisi
Wu, Jianyong
Chen, Jianghua
Peng, Wenhan
author_facet Xu, Jia
Yu, Yedong
Lv, Junhao
Yang, Sisi
Wu, Jianyong
Chen, Jianghua
Peng, Wenhan
author_sort Xu, Jia
collection PubMed
description BACKGROUND: Pneumocystis jirovecii pneumonia (PJP) is one of the common opportunistic infections diagnosed in kidney transplantation recipients. It is difficult to identify early by use of classic tools such as Grocott-Gomori stains and polymerase chain reaction (PCR). Metagenomic next-generation sequencing (mNGS) is accurate, unbiased, and sensitive, and is promising in PJP diagnosis. MATERIAL/METHODS: Data on kidney transplantation patients diagnosed with PJP were retrospectively analyzed. The sensitivity and specificity of different tools such as mNGS, laboratory tests, and Grocott-Gomori stains for PJP diagnosis were compared. All recipients were treated with trimethoprim-sulfamethoxazole (TMP-SMX). RESULTS: There were a total of 12 kidney transplantation recipients diagnosed with PJP based on mNGS in our center from January 01, 2020 to October 27, 2020. Highly variable numbers of sequence reads for P. jiroveci (19 to 1041285) showed diagnostic significance. Bronchoalveolar lavage fluid (BALF) samples were tested by Grocott-Gomori staining, with only 6 of 11 (54.5%) positive. Other routine laboratory tests like routine blood tests, blood biochemistry, procalcitonin (PCT), immune function, (1,3)-β-d-glucan (BG), serum galactomannan (GM), and C-reactive protein (CRP) showed even lower efficacy. TMP-SMX appeared to be the ideal therapy for kidney transplantation recipients with PJP. CONCLUSIONS: mNGS has utility in the diagnosis of PJP and mixed infections in kidney transplantation recipients, and TMP-SMX could be the ideal therapeutic drug for kidney transplantation recipients suffering from PJP.
format Online
Article
Text
id pubmed-8196548
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher International Scientific Literature, Inc.
record_format MEDLINE/PubMed
spelling pubmed-81965482021-06-25 Application of Metagenomic Next-Generation Sequencing to Diagnose Pneumocystis jirovecii Pneumonia in Kidney Transplantation Recipients Xu, Jia Yu, Yedong Lv, Junhao Yang, Sisi Wu, Jianyong Chen, Jianghua Peng, Wenhan Ann Transplant Original Paper BACKGROUND: Pneumocystis jirovecii pneumonia (PJP) is one of the common opportunistic infections diagnosed in kidney transplantation recipients. It is difficult to identify early by use of classic tools such as Grocott-Gomori stains and polymerase chain reaction (PCR). Metagenomic next-generation sequencing (mNGS) is accurate, unbiased, and sensitive, and is promising in PJP diagnosis. MATERIAL/METHODS: Data on kidney transplantation patients diagnosed with PJP were retrospectively analyzed. The sensitivity and specificity of different tools such as mNGS, laboratory tests, and Grocott-Gomori stains for PJP diagnosis were compared. All recipients were treated with trimethoprim-sulfamethoxazole (TMP-SMX). RESULTS: There were a total of 12 kidney transplantation recipients diagnosed with PJP based on mNGS in our center from January 01, 2020 to October 27, 2020. Highly variable numbers of sequence reads for P. jiroveci (19 to 1041285) showed diagnostic significance. Bronchoalveolar lavage fluid (BALF) samples were tested by Grocott-Gomori staining, with only 6 of 11 (54.5%) positive. Other routine laboratory tests like routine blood tests, blood biochemistry, procalcitonin (PCT), immune function, (1,3)-β-d-glucan (BG), serum galactomannan (GM), and C-reactive protein (CRP) showed even lower efficacy. TMP-SMX appeared to be the ideal therapy for kidney transplantation recipients with PJP. CONCLUSIONS: mNGS has utility in the diagnosis of PJP and mixed infections in kidney transplantation recipients, and TMP-SMX could be the ideal therapeutic drug for kidney transplantation recipients suffering from PJP. International Scientific Literature, Inc. 2021-06-08 /pmc/articles/PMC8196548/ /pubmed/34099614 http://dx.doi.org/10.12659/AOT.931059 Text en © Ann Transplant, 2021 https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Original Paper
Xu, Jia
Yu, Yedong
Lv, Junhao
Yang, Sisi
Wu, Jianyong
Chen, Jianghua
Peng, Wenhan
Application of Metagenomic Next-Generation Sequencing to Diagnose Pneumocystis jirovecii Pneumonia in Kidney Transplantation Recipients
title Application of Metagenomic Next-Generation Sequencing to Diagnose Pneumocystis jirovecii Pneumonia in Kidney Transplantation Recipients
title_full Application of Metagenomic Next-Generation Sequencing to Diagnose Pneumocystis jirovecii Pneumonia in Kidney Transplantation Recipients
title_fullStr Application of Metagenomic Next-Generation Sequencing to Diagnose Pneumocystis jirovecii Pneumonia in Kidney Transplantation Recipients
title_full_unstemmed Application of Metagenomic Next-Generation Sequencing to Diagnose Pneumocystis jirovecii Pneumonia in Kidney Transplantation Recipients
title_short Application of Metagenomic Next-Generation Sequencing to Diagnose Pneumocystis jirovecii Pneumonia in Kidney Transplantation Recipients
title_sort application of metagenomic next-generation sequencing to diagnose pneumocystis jirovecii pneumonia in kidney transplantation recipients
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196548/
https://www.ncbi.nlm.nih.gov/pubmed/34099614
http://dx.doi.org/10.12659/AOT.931059
work_keys_str_mv AT xujia applicationofmetagenomicnextgenerationsequencingtodiagnosepneumocystisjiroveciipneumoniainkidneytransplantationrecipients
AT yuyedong applicationofmetagenomicnextgenerationsequencingtodiagnosepneumocystisjiroveciipneumoniainkidneytransplantationrecipients
AT lvjunhao applicationofmetagenomicnextgenerationsequencingtodiagnosepneumocystisjiroveciipneumoniainkidneytransplantationrecipients
AT yangsisi applicationofmetagenomicnextgenerationsequencingtodiagnosepneumocystisjiroveciipneumoniainkidneytransplantationrecipients
AT wujianyong applicationofmetagenomicnextgenerationsequencingtodiagnosepneumocystisjiroveciipneumoniainkidneytransplantationrecipients
AT chenjianghua applicationofmetagenomicnextgenerationsequencingtodiagnosepneumocystisjiroveciipneumoniainkidneytransplantationrecipients
AT pengwenhan applicationofmetagenomicnextgenerationsequencingtodiagnosepneumocystisjiroveciipneumoniainkidneytransplantationrecipients