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Prolonged Transcriptional Consequences in Survivors of Sepsis

Survivors of sepsis often suffer from prolonged post-critical illness syndrome secondary to the immune system’s reprogramming. It is unclear if this process is static and pervasive due to methodological difficulties studying long-term outcomes of sepsis. The purpose of this study is to evaluate tran...

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Autores principales: Laudanski, Krzysztof, Soh, James, DiMeglio, Matthew, Sullivan, Kathleen E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196560/
https://www.ncbi.nlm.nih.gov/pubmed/34063857
http://dx.doi.org/10.3390/ijms22115422
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author Laudanski, Krzysztof
Soh, James
DiMeglio, Matthew
Sullivan, Kathleen E.
author_facet Laudanski, Krzysztof
Soh, James
DiMeglio, Matthew
Sullivan, Kathleen E.
author_sort Laudanski, Krzysztof
collection PubMed
description Survivors of sepsis often suffer from prolonged post-critical illness syndrome secondary to the immune system’s reprogramming. It is unclear if this process is static and pervasive due to methodological difficulties studying long-term outcomes of sepsis. The purpose of this study is to evaluate transcriptional profiles longitudinally in Drosophila melanogaster in the aftermath of sepsis to provide preliminary data for targets playing a role in post-sepsis immunostasis. Adult Drosophila melanogaster were infected with E. coli, and survivors were euthanized at 7, 14, and 21 days. Control flies were subjected to sham stress. Gene profiling was done with RNA-seq, and potential miRNA factors were computed. Profiling identified 55 unique genes at seven days, 61 unique genes at 14 days, and 78 genes at 21 days in sepsis survivors vs. sham control. Each post-sepsis timepoint had a distinctive transcriptional pattern with a signature related to oxidative stress at seven days, neuronal signal transduction at 14 days, and metabolism at 21 days. Several potential miRNA patterns were computed as potentially affecting several of the genes expressed in sepsis survivors. Our study demonstrated that post-sepsis changes in the transcriptome profile are dynamic and extend well into the Drosophila melanogaster natural life span.
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spelling pubmed-81965602021-06-13 Prolonged Transcriptional Consequences in Survivors of Sepsis Laudanski, Krzysztof Soh, James DiMeglio, Matthew Sullivan, Kathleen E. Int J Mol Sci Brief Report Survivors of sepsis often suffer from prolonged post-critical illness syndrome secondary to the immune system’s reprogramming. It is unclear if this process is static and pervasive due to methodological difficulties studying long-term outcomes of sepsis. The purpose of this study is to evaluate transcriptional profiles longitudinally in Drosophila melanogaster in the aftermath of sepsis to provide preliminary data for targets playing a role in post-sepsis immunostasis. Adult Drosophila melanogaster were infected with E. coli, and survivors were euthanized at 7, 14, and 21 days. Control flies were subjected to sham stress. Gene profiling was done with RNA-seq, and potential miRNA factors were computed. Profiling identified 55 unique genes at seven days, 61 unique genes at 14 days, and 78 genes at 21 days in sepsis survivors vs. sham control. Each post-sepsis timepoint had a distinctive transcriptional pattern with a signature related to oxidative stress at seven days, neuronal signal transduction at 14 days, and metabolism at 21 days. Several potential miRNA patterns were computed as potentially affecting several of the genes expressed in sepsis survivors. Our study demonstrated that post-sepsis changes in the transcriptome profile are dynamic and extend well into the Drosophila melanogaster natural life span. MDPI 2021-05-21 /pmc/articles/PMC8196560/ /pubmed/34063857 http://dx.doi.org/10.3390/ijms22115422 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Laudanski, Krzysztof
Soh, James
DiMeglio, Matthew
Sullivan, Kathleen E.
Prolonged Transcriptional Consequences in Survivors of Sepsis
title Prolonged Transcriptional Consequences in Survivors of Sepsis
title_full Prolonged Transcriptional Consequences in Survivors of Sepsis
title_fullStr Prolonged Transcriptional Consequences in Survivors of Sepsis
title_full_unstemmed Prolonged Transcriptional Consequences in Survivors of Sepsis
title_short Prolonged Transcriptional Consequences in Survivors of Sepsis
title_sort prolonged transcriptional consequences in survivors of sepsis
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196560/
https://www.ncbi.nlm.nih.gov/pubmed/34063857
http://dx.doi.org/10.3390/ijms22115422
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