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Prolonged Transcriptional Consequences in Survivors of Sepsis
Survivors of sepsis often suffer from prolonged post-critical illness syndrome secondary to the immune system’s reprogramming. It is unclear if this process is static and pervasive due to methodological difficulties studying long-term outcomes of sepsis. The purpose of this study is to evaluate tran...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196560/ https://www.ncbi.nlm.nih.gov/pubmed/34063857 http://dx.doi.org/10.3390/ijms22115422 |
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author | Laudanski, Krzysztof Soh, James DiMeglio, Matthew Sullivan, Kathleen E. |
author_facet | Laudanski, Krzysztof Soh, James DiMeglio, Matthew Sullivan, Kathleen E. |
author_sort | Laudanski, Krzysztof |
collection | PubMed |
description | Survivors of sepsis often suffer from prolonged post-critical illness syndrome secondary to the immune system’s reprogramming. It is unclear if this process is static and pervasive due to methodological difficulties studying long-term outcomes of sepsis. The purpose of this study is to evaluate transcriptional profiles longitudinally in Drosophila melanogaster in the aftermath of sepsis to provide preliminary data for targets playing a role in post-sepsis immunostasis. Adult Drosophila melanogaster were infected with E. coli, and survivors were euthanized at 7, 14, and 21 days. Control flies were subjected to sham stress. Gene profiling was done with RNA-seq, and potential miRNA factors were computed. Profiling identified 55 unique genes at seven days, 61 unique genes at 14 days, and 78 genes at 21 days in sepsis survivors vs. sham control. Each post-sepsis timepoint had a distinctive transcriptional pattern with a signature related to oxidative stress at seven days, neuronal signal transduction at 14 days, and metabolism at 21 days. Several potential miRNA patterns were computed as potentially affecting several of the genes expressed in sepsis survivors. Our study demonstrated that post-sepsis changes in the transcriptome profile are dynamic and extend well into the Drosophila melanogaster natural life span. |
format | Online Article Text |
id | pubmed-8196560 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81965602021-06-13 Prolonged Transcriptional Consequences in Survivors of Sepsis Laudanski, Krzysztof Soh, James DiMeglio, Matthew Sullivan, Kathleen E. Int J Mol Sci Brief Report Survivors of sepsis often suffer from prolonged post-critical illness syndrome secondary to the immune system’s reprogramming. It is unclear if this process is static and pervasive due to methodological difficulties studying long-term outcomes of sepsis. The purpose of this study is to evaluate transcriptional profiles longitudinally in Drosophila melanogaster in the aftermath of sepsis to provide preliminary data for targets playing a role in post-sepsis immunostasis. Adult Drosophila melanogaster were infected with E. coli, and survivors were euthanized at 7, 14, and 21 days. Control flies were subjected to sham stress. Gene profiling was done with RNA-seq, and potential miRNA factors were computed. Profiling identified 55 unique genes at seven days, 61 unique genes at 14 days, and 78 genes at 21 days in sepsis survivors vs. sham control. Each post-sepsis timepoint had a distinctive transcriptional pattern with a signature related to oxidative stress at seven days, neuronal signal transduction at 14 days, and metabolism at 21 days. Several potential miRNA patterns were computed as potentially affecting several of the genes expressed in sepsis survivors. Our study demonstrated that post-sepsis changes in the transcriptome profile are dynamic and extend well into the Drosophila melanogaster natural life span. MDPI 2021-05-21 /pmc/articles/PMC8196560/ /pubmed/34063857 http://dx.doi.org/10.3390/ijms22115422 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Laudanski, Krzysztof Soh, James DiMeglio, Matthew Sullivan, Kathleen E. Prolonged Transcriptional Consequences in Survivors of Sepsis |
title | Prolonged Transcriptional Consequences in Survivors of Sepsis |
title_full | Prolonged Transcriptional Consequences in Survivors of Sepsis |
title_fullStr | Prolonged Transcriptional Consequences in Survivors of Sepsis |
title_full_unstemmed | Prolonged Transcriptional Consequences in Survivors of Sepsis |
title_short | Prolonged Transcriptional Consequences in Survivors of Sepsis |
title_sort | prolonged transcriptional consequences in survivors of sepsis |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196560/ https://www.ncbi.nlm.nih.gov/pubmed/34063857 http://dx.doi.org/10.3390/ijms22115422 |
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