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Double-Edged Sword of Vitamin D3 Effects on Primary Neuronal Cultures in Hypoxic States

The use of vitamin D3 along with traditional therapy opens up new prospects for increasing the adaptive capacity of nerve cells to the effects of a wide range of stress factors, including hypoxia-ischemic processes. However, questions about prophylactic and therapeutic doses of vitamin D3 remain con...

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Autores principales: Loginova, Maria, Mishchenko, Tatiana, Savyuk, Maria, Guseva, Svetlana, Gavrish, Maria, Krivonosov, Mikhail, Ivanchenko, Mikhail, Fedotova, Julia, Vedunova, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196622/
https://www.ncbi.nlm.nih.gov/pubmed/34063823
http://dx.doi.org/10.3390/ijms22115417
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author Loginova, Maria
Mishchenko, Tatiana
Savyuk, Maria
Guseva, Svetlana
Gavrish, Maria
Krivonosov, Mikhail
Ivanchenko, Mikhail
Fedotova, Julia
Vedunova, Maria
author_facet Loginova, Maria
Mishchenko, Tatiana
Savyuk, Maria
Guseva, Svetlana
Gavrish, Maria
Krivonosov, Mikhail
Ivanchenko, Mikhail
Fedotova, Julia
Vedunova, Maria
author_sort Loginova, Maria
collection PubMed
description The use of vitamin D3 along with traditional therapy opens up new prospects for increasing the adaptive capacity of nerve cells to the effects of a wide range of stress factors, including hypoxia-ischemic processes. However, questions about prophylactic and therapeutic doses of vitamin D3 remain controversial. The purpose of our study was to analyze the effects of vitamin D3 at different concentrations on morpho-functional characteristics of neuron–glial networks in hypoxia modeling in vitro. We showed that a single administration of vitamin D3 at a high concentration (1 µM) in a normal state has no significant effect on the cell viability of primary neuronal cultures; however, it has a pronounced modulatory effect on the functional calcium activity of neuron–glial networks and causes destruction of the network response. Under hypoxia, the use of vitamin D3 (1 µM) leads to total cell death of primary neuronal cultures and complete negation of functional neural network activity. In contrast, application of lower concentrations of vitamin D3 (0.01 µM and 0.1 µM) caused a pronounced dose-dependent neuroprotective effect during the studied post-hypoxic period. While the use of vitamin D3 at a concentration of 0.1 µM maintained cell viability, preventive administration of 0.01 µM not only partially preserved the morphological integrity of primary neuronal cells but also maintained the functional structure and activity of neuron–glial networks in cultures. Possible molecular mechanisms of neuroprotective action of vitamin D3 can be associated with the increased expression level of transcription factor HIF-1α and maintaining the relationship between the levels of BDNF and TrkB expression in cells of primary neuronal cultures.
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spelling pubmed-81966222021-06-13 Double-Edged Sword of Vitamin D3 Effects on Primary Neuronal Cultures in Hypoxic States Loginova, Maria Mishchenko, Tatiana Savyuk, Maria Guseva, Svetlana Gavrish, Maria Krivonosov, Mikhail Ivanchenko, Mikhail Fedotova, Julia Vedunova, Maria Int J Mol Sci Article The use of vitamin D3 along with traditional therapy opens up new prospects for increasing the adaptive capacity of nerve cells to the effects of a wide range of stress factors, including hypoxia-ischemic processes. However, questions about prophylactic and therapeutic doses of vitamin D3 remain controversial. The purpose of our study was to analyze the effects of vitamin D3 at different concentrations on morpho-functional characteristics of neuron–glial networks in hypoxia modeling in vitro. We showed that a single administration of vitamin D3 at a high concentration (1 µM) in a normal state has no significant effect on the cell viability of primary neuronal cultures; however, it has a pronounced modulatory effect on the functional calcium activity of neuron–glial networks and causes destruction of the network response. Under hypoxia, the use of vitamin D3 (1 µM) leads to total cell death of primary neuronal cultures and complete negation of functional neural network activity. In contrast, application of lower concentrations of vitamin D3 (0.01 µM and 0.1 µM) caused a pronounced dose-dependent neuroprotective effect during the studied post-hypoxic period. While the use of vitamin D3 at a concentration of 0.1 µM maintained cell viability, preventive administration of 0.01 µM not only partially preserved the morphological integrity of primary neuronal cells but also maintained the functional structure and activity of neuron–glial networks in cultures. Possible molecular mechanisms of neuroprotective action of vitamin D3 can be associated with the increased expression level of transcription factor HIF-1α and maintaining the relationship between the levels of BDNF and TrkB expression in cells of primary neuronal cultures. MDPI 2021-05-21 /pmc/articles/PMC8196622/ /pubmed/34063823 http://dx.doi.org/10.3390/ijms22115417 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Loginova, Maria
Mishchenko, Tatiana
Savyuk, Maria
Guseva, Svetlana
Gavrish, Maria
Krivonosov, Mikhail
Ivanchenko, Mikhail
Fedotova, Julia
Vedunova, Maria
Double-Edged Sword of Vitamin D3 Effects on Primary Neuronal Cultures in Hypoxic States
title Double-Edged Sword of Vitamin D3 Effects on Primary Neuronal Cultures in Hypoxic States
title_full Double-Edged Sword of Vitamin D3 Effects on Primary Neuronal Cultures in Hypoxic States
title_fullStr Double-Edged Sword of Vitamin D3 Effects on Primary Neuronal Cultures in Hypoxic States
title_full_unstemmed Double-Edged Sword of Vitamin D3 Effects on Primary Neuronal Cultures in Hypoxic States
title_short Double-Edged Sword of Vitamin D3 Effects on Primary Neuronal Cultures in Hypoxic States
title_sort double-edged sword of vitamin d3 effects on primary neuronal cultures in hypoxic states
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196622/
https://www.ncbi.nlm.nih.gov/pubmed/34063823
http://dx.doi.org/10.3390/ijms22115417
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