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Quantification of Volatile Aldehydes Deriving from In Vitro Lipid Peroxidation in the Breath of Ventilated Patients

Exhaled aliphatic aldehydes were proposed as non-invasive biomarkers to detect increased lipid peroxidation in various diseases. As a prelude to clinical application of the multicapillary column–ion mobility spectrometry for the evaluation of aldehyde exhalation, we, therefore: (1) identified the mo...

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Autores principales: Müller-Wirtz, Lukas Martin, Kiefer, Daniel, Ruffing, Sven, Brausch, Timo, Hüppe, Tobias, Sessler, Daniel I., Volk, Thomas, Fink, Tobias, Kreuer, Sascha, Maurer, Felix
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196825/
https://www.ncbi.nlm.nih.gov/pubmed/34064214
http://dx.doi.org/10.3390/molecules26113089
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author Müller-Wirtz, Lukas Martin
Kiefer, Daniel
Ruffing, Sven
Brausch, Timo
Hüppe, Tobias
Sessler, Daniel I.
Volk, Thomas
Fink, Tobias
Kreuer, Sascha
Maurer, Felix
author_facet Müller-Wirtz, Lukas Martin
Kiefer, Daniel
Ruffing, Sven
Brausch, Timo
Hüppe, Tobias
Sessler, Daniel I.
Volk, Thomas
Fink, Tobias
Kreuer, Sascha
Maurer, Felix
author_sort Müller-Wirtz, Lukas Martin
collection PubMed
description Exhaled aliphatic aldehydes were proposed as non-invasive biomarkers to detect increased lipid peroxidation in various diseases. As a prelude to clinical application of the multicapillary column–ion mobility spectrometry for the evaluation of aldehyde exhalation, we, therefore: (1) identified the most abundant volatile aliphatic aldehydes originating from in vitro oxidation of various polyunsaturated fatty acids; (2) evaluated emittance of aldehydes from plastic parts of the breathing circuit; (3) conducted a pilot study for in vivo quantification of exhaled aldehydes in mechanically ventilated patients. Pentanal, hexanal, heptanal, and nonanal were quantifiable in the headspace of oxidizing polyunsaturated fatty acids, with pentanal and hexanal predominating. Plastic parts of the breathing circuit emitted hexanal, octanal, nonanal, and decanal, whereby nonanal and decanal were ubiquitous and pentanal or heptanal not being detected. Only pentanal was quantifiable in breath of mechanically ventilated surgical patients with a mean exhaled concentration of 13 ± 5 ppb. An explorative analysis suggested that pentanal exhalation is associated with mechanical power—a measure for the invasiveness of mechanical ventilation. In conclusion, exhaled pentanal is a promising non-invasive biomarker for lipid peroxidation inducing pathologies, and should be evaluated in future clinical studies, particularly for detection of lung injury.
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spelling pubmed-81968252021-06-13 Quantification of Volatile Aldehydes Deriving from In Vitro Lipid Peroxidation in the Breath of Ventilated Patients Müller-Wirtz, Lukas Martin Kiefer, Daniel Ruffing, Sven Brausch, Timo Hüppe, Tobias Sessler, Daniel I. Volk, Thomas Fink, Tobias Kreuer, Sascha Maurer, Felix Molecules Article Exhaled aliphatic aldehydes were proposed as non-invasive biomarkers to detect increased lipid peroxidation in various diseases. As a prelude to clinical application of the multicapillary column–ion mobility spectrometry for the evaluation of aldehyde exhalation, we, therefore: (1) identified the most abundant volatile aliphatic aldehydes originating from in vitro oxidation of various polyunsaturated fatty acids; (2) evaluated emittance of aldehydes from plastic parts of the breathing circuit; (3) conducted a pilot study for in vivo quantification of exhaled aldehydes in mechanically ventilated patients. Pentanal, hexanal, heptanal, and nonanal were quantifiable in the headspace of oxidizing polyunsaturated fatty acids, with pentanal and hexanal predominating. Plastic parts of the breathing circuit emitted hexanal, octanal, nonanal, and decanal, whereby nonanal and decanal were ubiquitous and pentanal or heptanal not being detected. Only pentanal was quantifiable in breath of mechanically ventilated surgical patients with a mean exhaled concentration of 13 ± 5 ppb. An explorative analysis suggested that pentanal exhalation is associated with mechanical power—a measure for the invasiveness of mechanical ventilation. In conclusion, exhaled pentanal is a promising non-invasive biomarker for lipid peroxidation inducing pathologies, and should be evaluated in future clinical studies, particularly for detection of lung injury. MDPI 2021-05-21 /pmc/articles/PMC8196825/ /pubmed/34064214 http://dx.doi.org/10.3390/molecules26113089 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Müller-Wirtz, Lukas Martin
Kiefer, Daniel
Ruffing, Sven
Brausch, Timo
Hüppe, Tobias
Sessler, Daniel I.
Volk, Thomas
Fink, Tobias
Kreuer, Sascha
Maurer, Felix
Quantification of Volatile Aldehydes Deriving from In Vitro Lipid Peroxidation in the Breath of Ventilated Patients
title Quantification of Volatile Aldehydes Deriving from In Vitro Lipid Peroxidation in the Breath of Ventilated Patients
title_full Quantification of Volatile Aldehydes Deriving from In Vitro Lipid Peroxidation in the Breath of Ventilated Patients
title_fullStr Quantification of Volatile Aldehydes Deriving from In Vitro Lipid Peroxidation in the Breath of Ventilated Patients
title_full_unstemmed Quantification of Volatile Aldehydes Deriving from In Vitro Lipid Peroxidation in the Breath of Ventilated Patients
title_short Quantification of Volatile Aldehydes Deriving from In Vitro Lipid Peroxidation in the Breath of Ventilated Patients
title_sort quantification of volatile aldehydes deriving from in vitro lipid peroxidation in the breath of ventilated patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196825/
https://www.ncbi.nlm.nih.gov/pubmed/34064214
http://dx.doi.org/10.3390/molecules26113089
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