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Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis

Cholangiocarcinoma (CCA) is a malignancy arising from cholangiocytes. Currently, the treatment and prognosis for CCA are mostly poor. Recently, we have reported that coiled-coil domain containing 25 (CCDC25) protein level in the sera may be a diagnostic marker for CCA. Subsequently, we identified th...

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Autores principales: Siriphak, Saeranee, Chanakankun, Ravinnipa, Proungvitaya, Tanakorn, Roytrakul, Sittiruk, Tummanatsakun, Doungdean, Seubwai, Wunchana, Wongwattanakul, Molin, Proungvitaya, Siriporn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196963/
https://www.ncbi.nlm.nih.gov/pubmed/34067437
http://dx.doi.org/10.3390/molecules26113105
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author Siriphak, Saeranee
Chanakankun, Ravinnipa
Proungvitaya, Tanakorn
Roytrakul, Sittiruk
Tummanatsakun, Doungdean
Seubwai, Wunchana
Wongwattanakul, Molin
Proungvitaya, Siriporn
author_facet Siriphak, Saeranee
Chanakankun, Ravinnipa
Proungvitaya, Tanakorn
Roytrakul, Sittiruk
Tummanatsakun, Doungdean
Seubwai, Wunchana
Wongwattanakul, Molin
Proungvitaya, Siriporn
author_sort Siriphak, Saeranee
collection PubMed
description Cholangiocarcinoma (CCA) is a malignancy arising from cholangiocytes. Currently, the treatment and prognosis for CCA are mostly poor. Recently, we have reported that coiled-coil domain containing 25 (CCDC25) protein level in the sera may be a diagnostic marker for CCA. Subsequently, we identified three binding proteins of CCDC25 and found that kallikrein-11 (KLK11) expression was highest among those binding proteins. In this study, we investigated CCDC25 and KLK11 expression in CCA and adjacent normal tissues (n = 18) using immunohistochemistry. The results demonstrated that the expressions of CCDC25 and KLK11 in CCA tissues were both significantly higher than the adjacent tissues (p < 0.001 and p = 0.001, respectively). Then, using GEPIA bioinformatics analysis, KLK11 mRNA was significantly overexpressed in CCA tumor tissues compared with normal tissues (p < 0.05). Moreover, CCDC25 expression was positively correlated with KLK11 expression in CCA with lymph node metastasis (p = 0.028, r = 0.593). An analysis for the interaction of KLK11 with CCDC25 and other proteins, using STRING version 11.0, revealed that CCDC25 and KLK11 correlated with metastasis-related proteins. In addition, Kaplan-Meier survival curve analysis revealed that a high expression of KLK11 was associated with the poor prognosis of CCA. In conclusion, KLK11 is, as a binding protein for CCDC25, possibly involved in the metastatic process of CCA. KLK11 may be used as a prognostic marker for CCA.
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spelling pubmed-81969632021-06-13 Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis Siriphak, Saeranee Chanakankun, Ravinnipa Proungvitaya, Tanakorn Roytrakul, Sittiruk Tummanatsakun, Doungdean Seubwai, Wunchana Wongwattanakul, Molin Proungvitaya, Siriporn Molecules Article Cholangiocarcinoma (CCA) is a malignancy arising from cholangiocytes. Currently, the treatment and prognosis for CCA are mostly poor. Recently, we have reported that coiled-coil domain containing 25 (CCDC25) protein level in the sera may be a diagnostic marker for CCA. Subsequently, we identified three binding proteins of CCDC25 and found that kallikrein-11 (KLK11) expression was highest among those binding proteins. In this study, we investigated CCDC25 and KLK11 expression in CCA and adjacent normal tissues (n = 18) using immunohistochemistry. The results demonstrated that the expressions of CCDC25 and KLK11 in CCA tissues were both significantly higher than the adjacent tissues (p < 0.001 and p = 0.001, respectively). Then, using GEPIA bioinformatics analysis, KLK11 mRNA was significantly overexpressed in CCA tumor tissues compared with normal tissues (p < 0.05). Moreover, CCDC25 expression was positively correlated with KLK11 expression in CCA with lymph node metastasis (p = 0.028, r = 0.593). An analysis for the interaction of KLK11 with CCDC25 and other proteins, using STRING version 11.0, revealed that CCDC25 and KLK11 correlated with metastasis-related proteins. In addition, Kaplan-Meier survival curve analysis revealed that a high expression of KLK11 was associated with the poor prognosis of CCA. In conclusion, KLK11 is, as a binding protein for CCDC25, possibly involved in the metastatic process of CCA. KLK11 may be used as a prognostic marker for CCA. MDPI 2021-05-22 /pmc/articles/PMC8196963/ /pubmed/34067437 http://dx.doi.org/10.3390/molecules26113105 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Siriphak, Saeranee
Chanakankun, Ravinnipa
Proungvitaya, Tanakorn
Roytrakul, Sittiruk
Tummanatsakun, Doungdean
Seubwai, Wunchana
Wongwattanakul, Molin
Proungvitaya, Siriporn
Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis
title Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis
title_full Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis
title_fullStr Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis
title_full_unstemmed Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis
title_short Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis
title_sort kallikrein-11, in association with coiled-coil domain containing 25, as a potential prognostic marker for cholangiocarcinoma with lymph node metastasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196963/
https://www.ncbi.nlm.nih.gov/pubmed/34067437
http://dx.doi.org/10.3390/molecules26113105
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