Cargando…
Prognostic Role of Tumoral PD-L1 and IDO1 Expression, and Intratumoral CD8+ and FoxP3+ Lymphocyte Infiltrates in 132 Primary Cutaneous Merkel Cell Carcinomas
The association of immune markers and clinicopathologic features and patient outcome has not been extensively studied in Merkel cell carcinoma (MCC). We correlated tumoral PD-L1 and IDO1 expression, and intratumoral CD8+ and FoxP3+ lymphocytes count with clinicopathologic variables, Merkel cell poly...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197111/ https://www.ncbi.nlm.nih.gov/pubmed/34071045 http://dx.doi.org/10.3390/ijms22115489 |
_version_ | 1783706844315779072 |
---|---|
author | Donizy, Piotr Wu, Cheng-Lin Kopczynski, Janusz Pieniazek, Malgorzata Biecek, Przemyslaw Ryś, Janusz Hoang, Mai P. |
author_facet | Donizy, Piotr Wu, Cheng-Lin Kopczynski, Janusz Pieniazek, Malgorzata Biecek, Przemyslaw Ryś, Janusz Hoang, Mai P. |
author_sort | Donizy, Piotr |
collection | PubMed |
description | The association of immune markers and clinicopathologic features and patient outcome has not been extensively studied in Merkel cell carcinoma (MCC). We correlated tumoral PD-L1 and IDO1 expression, and intratumoral CD8+ and FoxP3+ lymphocytes count with clinicopathologic variables, Merkel cell polyomavirus (MCPyV) status, and patient outcomes in a series of 132 MCC. By univariate analyses, tumoral PD-L1 expression >1% and combined tumoral PD-L1 >1% and high intratumoral FoxP3+ lymphocyte count correlated with improved overall survival (OS) (p = 0.016, 0.0072), MCC-specific survival (MSS) (p = 0.019, 0.017), and progression-free survival (PFS) (p = 0.043, 0.004, respectively). High intratumoral CD8+ and FoxP3+ lymphocyte count correlated with longer MSS (p = 0.036) and improved PFS (p = 0.047), respectively. Ulceration correlated with worse OS and worse MSS. Age, male gender, and higher stage (3 and 4) significantly correlated with worse survival. MCPyV positivity correlated with immune response. By multivariate analyses, only ulceration and age remained as independent predictors of worse OS; gender and stage remained for shorter PFS. Tumoral PD-L1 expression and increased density of intratumoral CD8+ lymphocytes and FoxP+ lymphocytes may represent favorable prognosticators in a subset of MCCs. Tumoral PD-L1 expression correlated with intratumoral CD8+ and FoxP3+ lymphocytes, which is supportive of an adaptive immune response. |
format | Online Article Text |
id | pubmed-8197111 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81971112021-06-13 Prognostic Role of Tumoral PD-L1 and IDO1 Expression, and Intratumoral CD8+ and FoxP3+ Lymphocyte Infiltrates in 132 Primary Cutaneous Merkel Cell Carcinomas Donizy, Piotr Wu, Cheng-Lin Kopczynski, Janusz Pieniazek, Malgorzata Biecek, Przemyslaw Ryś, Janusz Hoang, Mai P. Int J Mol Sci Article The association of immune markers and clinicopathologic features and patient outcome has not been extensively studied in Merkel cell carcinoma (MCC). We correlated tumoral PD-L1 and IDO1 expression, and intratumoral CD8+ and FoxP3+ lymphocytes count with clinicopathologic variables, Merkel cell polyomavirus (MCPyV) status, and patient outcomes in a series of 132 MCC. By univariate analyses, tumoral PD-L1 expression >1% and combined tumoral PD-L1 >1% and high intratumoral FoxP3+ lymphocyte count correlated with improved overall survival (OS) (p = 0.016, 0.0072), MCC-specific survival (MSS) (p = 0.019, 0.017), and progression-free survival (PFS) (p = 0.043, 0.004, respectively). High intratumoral CD8+ and FoxP3+ lymphocyte count correlated with longer MSS (p = 0.036) and improved PFS (p = 0.047), respectively. Ulceration correlated with worse OS and worse MSS. Age, male gender, and higher stage (3 and 4) significantly correlated with worse survival. MCPyV positivity correlated with immune response. By multivariate analyses, only ulceration and age remained as independent predictors of worse OS; gender and stage remained for shorter PFS. Tumoral PD-L1 expression and increased density of intratumoral CD8+ lymphocytes and FoxP+ lymphocytes may represent favorable prognosticators in a subset of MCCs. Tumoral PD-L1 expression correlated with intratumoral CD8+ and FoxP3+ lymphocytes, which is supportive of an adaptive immune response. MDPI 2021-05-23 /pmc/articles/PMC8197111/ /pubmed/34071045 http://dx.doi.org/10.3390/ijms22115489 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Donizy, Piotr Wu, Cheng-Lin Kopczynski, Janusz Pieniazek, Malgorzata Biecek, Przemyslaw Ryś, Janusz Hoang, Mai P. Prognostic Role of Tumoral PD-L1 and IDO1 Expression, and Intratumoral CD8+ and FoxP3+ Lymphocyte Infiltrates in 132 Primary Cutaneous Merkel Cell Carcinomas |
title | Prognostic Role of Tumoral PD-L1 and IDO1 Expression, and Intratumoral CD8+ and FoxP3+ Lymphocyte Infiltrates in 132 Primary Cutaneous Merkel Cell Carcinomas |
title_full | Prognostic Role of Tumoral PD-L1 and IDO1 Expression, and Intratumoral CD8+ and FoxP3+ Lymphocyte Infiltrates in 132 Primary Cutaneous Merkel Cell Carcinomas |
title_fullStr | Prognostic Role of Tumoral PD-L1 and IDO1 Expression, and Intratumoral CD8+ and FoxP3+ Lymphocyte Infiltrates in 132 Primary Cutaneous Merkel Cell Carcinomas |
title_full_unstemmed | Prognostic Role of Tumoral PD-L1 and IDO1 Expression, and Intratumoral CD8+ and FoxP3+ Lymphocyte Infiltrates in 132 Primary Cutaneous Merkel Cell Carcinomas |
title_short | Prognostic Role of Tumoral PD-L1 and IDO1 Expression, and Intratumoral CD8+ and FoxP3+ Lymphocyte Infiltrates in 132 Primary Cutaneous Merkel Cell Carcinomas |
title_sort | prognostic role of tumoral pd-l1 and ido1 expression, and intratumoral cd8+ and foxp3+ lymphocyte infiltrates in 132 primary cutaneous merkel cell carcinomas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197111/ https://www.ncbi.nlm.nih.gov/pubmed/34071045 http://dx.doi.org/10.3390/ijms22115489 |
work_keys_str_mv | AT donizypiotr prognosticroleoftumoralpdl1andido1expressionandintratumoralcd8andfoxp3lymphocyteinfiltratesin132primarycutaneousmerkelcellcarcinomas AT wuchenglin prognosticroleoftumoralpdl1andido1expressionandintratumoralcd8andfoxp3lymphocyteinfiltratesin132primarycutaneousmerkelcellcarcinomas AT kopczynskijanusz prognosticroleoftumoralpdl1andido1expressionandintratumoralcd8andfoxp3lymphocyteinfiltratesin132primarycutaneousmerkelcellcarcinomas AT pieniazekmalgorzata prognosticroleoftumoralpdl1andido1expressionandintratumoralcd8andfoxp3lymphocyteinfiltratesin132primarycutaneousmerkelcellcarcinomas AT biecekprzemyslaw prognosticroleoftumoralpdl1andido1expressionandintratumoralcd8andfoxp3lymphocyteinfiltratesin132primarycutaneousmerkelcellcarcinomas AT rysjanusz prognosticroleoftumoralpdl1andido1expressionandintratumoralcd8andfoxp3lymphocyteinfiltratesin132primarycutaneousmerkelcellcarcinomas AT hoangmaip prognosticroleoftumoralpdl1andido1expressionandintratumoralcd8andfoxp3lymphocyteinfiltratesin132primarycutaneousmerkelcellcarcinomas |