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Microfabricated potentiometric sensor for personalized methacholine challenge tests during the COVID-19 pandemic
The methacholine challenge test is considered to be the gold standard bronchoprovocation test used to diagnose asthma, and this test is always performed in pulmonary function labs or doctors’ offices. Methacholine (MCH) acts by inducing airway tightening/bronchoconstriction, and more importantly, MC...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197613/ https://www.ncbi.nlm.nih.gov/pubmed/34166943 http://dx.doi.org/10.1016/j.bios.2021.113439 |
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author | ElDin, Norhan Badr El-Rahman, Mohamed K.Abd Zaazaa, Hala E. Moustafa, Azza A. Hassan, Said A. |
author_facet | ElDin, Norhan Badr El-Rahman, Mohamed K.Abd Zaazaa, Hala E. Moustafa, Azza A. Hassan, Said A. |
author_sort | ElDin, Norhan Badr |
collection | PubMed |
description | The methacholine challenge test is considered to be the gold standard bronchoprovocation test used to diagnose asthma, and this test is always performed in pulmonary function labs or doctors’ offices. Methacholine (MCH) acts by inducing airway tightening/bronchoconstriction, and more importantly, MCH is hydrolyzed by cholinesterase enzyme (ChE). Recently, the American Thoracic Society raised concerns about pulmonary function testing during the COVID-19 pandemic due to recently reported correlation between cholinesterase and COVID-19 pneumonia severity/mortality, and it was shown that cholinesterase levels are reduced in the acute phase of severe COVID-19 pneumonia. This work describes the microfabrication of potentiometric sensors using copper as the substrate and chemically polymerized graphene nanocomposites as the transducing layer for tracking the kinetics of MCH enzymatic degradation in real blood samples. The in-vitro estimation of the characteristic parameters of the MCH metabolism [Michaelis–Menten constant (K(m)) and reaction velocity (V(max))] were found to be 241.041 μM and 56.8 μM/min, respectively. The proposed sensor is designed to be used as a companion diagnostic device that can (i) answer questions about patient eligibility to perform methacholine challenge tests, (ii) individualize/personalize medical dosing of methacholine, (iii) provide portable and inexpensive devices allowing automated readouts without the need for operator intervention (iv) recommend therapeutic interventions including intensive care during early stages and reflecting the disease state of COVID-19 pneumonia. We hope that this methacholine electrochemical sensor will help in assaying ChE activity in a “timely” manner and predict the severity and prognosis of COVID-19 to improve treatment outcomes and decrease mortality. |
format | Online Article Text |
id | pubmed-8197613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81976132021-06-15 Microfabricated potentiometric sensor for personalized methacholine challenge tests during the COVID-19 pandemic ElDin, Norhan Badr El-Rahman, Mohamed K.Abd Zaazaa, Hala E. Moustafa, Azza A. Hassan, Said A. Biosens Bioelectron Article The methacholine challenge test is considered to be the gold standard bronchoprovocation test used to diagnose asthma, and this test is always performed in pulmonary function labs or doctors’ offices. Methacholine (MCH) acts by inducing airway tightening/bronchoconstriction, and more importantly, MCH is hydrolyzed by cholinesterase enzyme (ChE). Recently, the American Thoracic Society raised concerns about pulmonary function testing during the COVID-19 pandemic due to recently reported correlation between cholinesterase and COVID-19 pneumonia severity/mortality, and it was shown that cholinesterase levels are reduced in the acute phase of severe COVID-19 pneumonia. This work describes the microfabrication of potentiometric sensors using copper as the substrate and chemically polymerized graphene nanocomposites as the transducing layer for tracking the kinetics of MCH enzymatic degradation in real blood samples. The in-vitro estimation of the characteristic parameters of the MCH metabolism [Michaelis–Menten constant (K(m)) and reaction velocity (V(max))] were found to be 241.041 μM and 56.8 μM/min, respectively. The proposed sensor is designed to be used as a companion diagnostic device that can (i) answer questions about patient eligibility to perform methacholine challenge tests, (ii) individualize/personalize medical dosing of methacholine, (iii) provide portable and inexpensive devices allowing automated readouts without the need for operator intervention (iv) recommend therapeutic interventions including intensive care during early stages and reflecting the disease state of COVID-19 pneumonia. We hope that this methacholine electrochemical sensor will help in assaying ChE activity in a “timely” manner and predict the severity and prognosis of COVID-19 to improve treatment outcomes and decrease mortality. Elsevier B.V. 2021-10-15 2021-06-12 /pmc/articles/PMC8197613/ /pubmed/34166943 http://dx.doi.org/10.1016/j.bios.2021.113439 Text en © 2021 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article ElDin, Norhan Badr El-Rahman, Mohamed K.Abd Zaazaa, Hala E. Moustafa, Azza A. Hassan, Said A. Microfabricated potentiometric sensor for personalized methacholine challenge tests during the COVID-19 pandemic |
title | Microfabricated potentiometric sensor for personalized methacholine challenge tests during the COVID-19 pandemic |
title_full | Microfabricated potentiometric sensor for personalized methacholine challenge tests during the COVID-19 pandemic |
title_fullStr | Microfabricated potentiometric sensor for personalized methacholine challenge tests during the COVID-19 pandemic |
title_full_unstemmed | Microfabricated potentiometric sensor for personalized methacholine challenge tests during the COVID-19 pandemic |
title_short | Microfabricated potentiometric sensor for personalized methacholine challenge tests during the COVID-19 pandemic |
title_sort | microfabricated potentiometric sensor for personalized methacholine challenge tests during the covid-19 pandemic |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197613/ https://www.ncbi.nlm.nih.gov/pubmed/34166943 http://dx.doi.org/10.1016/j.bios.2021.113439 |
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