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Influence of Contact Lens Materials and Cleaning Procedures on Bacterial Adhesion and Biofilm Formation

PURPOSE: The present study aimed to compare the existing soft contact lens (CL) materials regarding their influence on bacterial biofilm formation and adhesion susceptibility. Then, the study was designed to investigate the effectiveness of various disinfecting solutions and evaluate the ability of...

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Autores principales: Mordmuang, Auemphon, Udomwech, Lunla, Karnjana, Kulwadee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197623/
https://www.ncbi.nlm.nih.gov/pubmed/34135567
http://dx.doi.org/10.2147/OPTH.S310862
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author Mordmuang, Auemphon
Udomwech, Lunla
Karnjana, Kulwadee
author_facet Mordmuang, Auemphon
Udomwech, Lunla
Karnjana, Kulwadee
author_sort Mordmuang, Auemphon
collection PubMed
description PURPOSE: The present study aimed to compare the existing soft contact lens (CL) materials regarding their influence on bacterial biofilm formation and adhesion susceptibility. Then, the study was designed to investigate the effectiveness of various disinfecting solutions and evaluate the ability of cleaning regimens in terms of anti-bacterial adhesion and biofilm removal on different soft CL materials. METHODS: Bacterial biofilm formation on CLs was evaluated by biomass assay. Adhesion assay and standard plate count were carried out at time-interval periods within 24 h. Various CL disinfecting procedures were assessed for their efficacy to remove biofilm and reduce bacterial adhesion. Scanning electron microscopy (SEM) was performed for the morphological assessment of bacterial biofilm. RESULTS: Printed hydroxyethyl methacrylate (HEMA) CLs significantly demonstrated more biofilm staining and bacterial attachment when compared with non-printed HEMA CLs, while the Filcon II 3 and Nesofilcon A CLs possessed less biofilm biomass and adherent cells. Staphylococcus aureus and Pseudomonas aeruginosa represented the highest biofilm producing bacteria on HEMA-based materials in this study. The disinfecting regimen with the highest efficacy was the two-step system, first using multipurpose disinfecting solution containing edetate disodium and sorbic acid (MPDS+EDSA), followed by soaking in multipurpose solution (MPS). The regimen demonstrated the greatest effect against the pre-formed biofilm and the adhesion activity of S. aureus and P. aeruginosa on the soft CLs. The SEM micrographs confirmed the morphological changes of bacterial biofilm after disinfecting and revealed that the two-step system treated CLs displayed less adherent bacteria. CONCLUSION: HEMA-based soft CLs may facilitate bacterial biofilm formation and adhesion capability. The two-step system was the most effective regimen for biofilm removal, where the soaking period in the disinfecting solution of the no-rub regimen should last more than 6 h to remove pre-formed biofilm.
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spelling pubmed-81976232021-06-15 Influence of Contact Lens Materials and Cleaning Procedures on Bacterial Adhesion and Biofilm Formation Mordmuang, Auemphon Udomwech, Lunla Karnjana, Kulwadee Clin Ophthalmol Original Research PURPOSE: The present study aimed to compare the existing soft contact lens (CL) materials regarding their influence on bacterial biofilm formation and adhesion susceptibility. Then, the study was designed to investigate the effectiveness of various disinfecting solutions and evaluate the ability of cleaning regimens in terms of anti-bacterial adhesion and biofilm removal on different soft CL materials. METHODS: Bacterial biofilm formation on CLs was evaluated by biomass assay. Adhesion assay and standard plate count were carried out at time-interval periods within 24 h. Various CL disinfecting procedures were assessed for their efficacy to remove biofilm and reduce bacterial adhesion. Scanning electron microscopy (SEM) was performed for the morphological assessment of bacterial biofilm. RESULTS: Printed hydroxyethyl methacrylate (HEMA) CLs significantly demonstrated more biofilm staining and bacterial attachment when compared with non-printed HEMA CLs, while the Filcon II 3 and Nesofilcon A CLs possessed less biofilm biomass and adherent cells. Staphylococcus aureus and Pseudomonas aeruginosa represented the highest biofilm producing bacteria on HEMA-based materials in this study. The disinfecting regimen with the highest efficacy was the two-step system, first using multipurpose disinfecting solution containing edetate disodium and sorbic acid (MPDS+EDSA), followed by soaking in multipurpose solution (MPS). The regimen demonstrated the greatest effect against the pre-formed biofilm and the adhesion activity of S. aureus and P. aeruginosa on the soft CLs. The SEM micrographs confirmed the morphological changes of bacterial biofilm after disinfecting and revealed that the two-step system treated CLs displayed less adherent bacteria. CONCLUSION: HEMA-based soft CLs may facilitate bacterial biofilm formation and adhesion capability. The two-step system was the most effective regimen for biofilm removal, where the soaking period in the disinfecting solution of the no-rub regimen should last more than 6 h to remove pre-formed biofilm. Dove 2021-06-08 /pmc/articles/PMC8197623/ /pubmed/34135567 http://dx.doi.org/10.2147/OPTH.S310862 Text en © 2021 Mordmuang et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Mordmuang, Auemphon
Udomwech, Lunla
Karnjana, Kulwadee
Influence of Contact Lens Materials and Cleaning Procedures on Bacterial Adhesion and Biofilm Formation
title Influence of Contact Lens Materials and Cleaning Procedures on Bacterial Adhesion and Biofilm Formation
title_full Influence of Contact Lens Materials and Cleaning Procedures on Bacterial Adhesion and Biofilm Formation
title_fullStr Influence of Contact Lens Materials and Cleaning Procedures on Bacterial Adhesion and Biofilm Formation
title_full_unstemmed Influence of Contact Lens Materials and Cleaning Procedures on Bacterial Adhesion and Biofilm Formation
title_short Influence of Contact Lens Materials and Cleaning Procedures on Bacterial Adhesion and Biofilm Formation
title_sort influence of contact lens materials and cleaning procedures on bacterial adhesion and biofilm formation
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197623/
https://www.ncbi.nlm.nih.gov/pubmed/34135567
http://dx.doi.org/10.2147/OPTH.S310862
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