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Novel Methods to Mobilize, Isolate, and Expand Mesenchymal Stem Cells
Numerous studies demonstrate the essential role of mesenchymal stem cells (MSCs) in the treatment of metabolic and inflammatory diseases, as these cells are known to modulate humoral and cellular immune responses. In this manuscript, we efficiently present two novel approaches to obtain MSCs from eq...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197893/ https://www.ncbi.nlm.nih.gov/pubmed/34072061 http://dx.doi.org/10.3390/ijms22115728 |
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author | Vieira, Cristiano P. McCarrel, Taralyn M. Grant, Maria B. |
author_facet | Vieira, Cristiano P. McCarrel, Taralyn M. Grant, Maria B. |
author_sort | Vieira, Cristiano P. |
collection | PubMed |
description | Numerous studies demonstrate the essential role of mesenchymal stem cells (MSCs) in the treatment of metabolic and inflammatory diseases, as these cells are known to modulate humoral and cellular immune responses. In this manuscript, we efficiently present two novel approaches to obtain MSCs from equine or human sources. In our first approach, we used electro-acupuncture as previously described by our group to mobilize MSCs into the peripheral blood of horses. For equine MSC collection, culture, and expansion, we used the Miltenyi Biotec CliniMACS Prodigy system of automated cell manufacturing. Using this system, we were able to generate appoximately 100 MSC colonies that exhibit surface marker expression of CD105 (92%), CD90 (85%), and CD73 (88%) within seven days of blood collection. Our second approach utilized the iPSC embryoid bodies from healthy or diabetic subjects where the iPSCs were cultured in standard media (endothelial + mesoderm basal media). After 21 days, the cells were FACS sorted and exhibited surface marker expression of CD105, CD90, and CD73. Both the equine cells and the human iPSC-derived MSCs were able to differentiate into adipogenic, osteogenic, and chondrogenic lineages. Both methods described simple and highly efficient methods to produce cells with surface markers phenotypically considered as MSCs and may, in the future, facilitate rapid production of MSCs with therapeutic potential. |
format | Online Article Text |
id | pubmed-8197893 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81978932021-06-14 Novel Methods to Mobilize, Isolate, and Expand Mesenchymal Stem Cells Vieira, Cristiano P. McCarrel, Taralyn M. Grant, Maria B. Int J Mol Sci Article Numerous studies demonstrate the essential role of mesenchymal stem cells (MSCs) in the treatment of metabolic and inflammatory diseases, as these cells are known to modulate humoral and cellular immune responses. In this manuscript, we efficiently present two novel approaches to obtain MSCs from equine or human sources. In our first approach, we used electro-acupuncture as previously described by our group to mobilize MSCs into the peripheral blood of horses. For equine MSC collection, culture, and expansion, we used the Miltenyi Biotec CliniMACS Prodigy system of automated cell manufacturing. Using this system, we were able to generate appoximately 100 MSC colonies that exhibit surface marker expression of CD105 (92%), CD90 (85%), and CD73 (88%) within seven days of blood collection. Our second approach utilized the iPSC embryoid bodies from healthy or diabetic subjects where the iPSCs were cultured in standard media (endothelial + mesoderm basal media). After 21 days, the cells were FACS sorted and exhibited surface marker expression of CD105, CD90, and CD73. Both the equine cells and the human iPSC-derived MSCs were able to differentiate into adipogenic, osteogenic, and chondrogenic lineages. Both methods described simple and highly efficient methods to produce cells with surface markers phenotypically considered as MSCs and may, in the future, facilitate rapid production of MSCs with therapeutic potential. MDPI 2021-05-27 /pmc/articles/PMC8197893/ /pubmed/34072061 http://dx.doi.org/10.3390/ijms22115728 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vieira, Cristiano P. McCarrel, Taralyn M. Grant, Maria B. Novel Methods to Mobilize, Isolate, and Expand Mesenchymal Stem Cells |
title | Novel Methods to Mobilize, Isolate, and Expand Mesenchymal Stem Cells |
title_full | Novel Methods to Mobilize, Isolate, and Expand Mesenchymal Stem Cells |
title_fullStr | Novel Methods to Mobilize, Isolate, and Expand Mesenchymal Stem Cells |
title_full_unstemmed | Novel Methods to Mobilize, Isolate, and Expand Mesenchymal Stem Cells |
title_short | Novel Methods to Mobilize, Isolate, and Expand Mesenchymal Stem Cells |
title_sort | novel methods to mobilize, isolate, and expand mesenchymal stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197893/ https://www.ncbi.nlm.nih.gov/pubmed/34072061 http://dx.doi.org/10.3390/ijms22115728 |
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