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Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics

Recently, medaka has been used as a model organism in various research fields. However, even though it possesses several advantages over zebrafish, fewer studies were done in medaka compared to zebrafish, especially with regard to its behavior. Thus, to provide more information regarding its behavio...

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Autores principales: Audira, Gilbert, Siregar, Petrus, Chen, Kelvin H.-C., Roldan, Marri Jmelou M., Huang, Jong-Chin, Lai, Hong-Thih, Hsiao, Chung-Der
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197923/
https://www.ncbi.nlm.nih.gov/pubmed/34073632
http://dx.doi.org/10.3390/ijms22115686
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author Audira, Gilbert
Siregar, Petrus
Chen, Kelvin H.-C.
Roldan, Marri Jmelou M.
Huang, Jong-Chin
Lai, Hong-Thih
Hsiao, Chung-Der
author_facet Audira, Gilbert
Siregar, Petrus
Chen, Kelvin H.-C.
Roldan, Marri Jmelou M.
Huang, Jong-Chin
Lai, Hong-Thih
Hsiao, Chung-Der
author_sort Audira, Gilbert
collection PubMed
description Recently, medaka has been used as a model organism in various research fields. However, even though it possesses several advantages over zebrafish, fewer studies were done in medaka compared to zebrafish, especially with regard to its behavior. Thus, to provide more information regarding its behavior and to demonstrate the behavioral differences between several species of medaka, we compared the behavioral performance and biomarker expression in the brain between four medaka fishes, Oryzias latipes, Oryzias dancena, Oryzias woworae, and Oryzias sinensis. We found that each medaka species explicitly exhibited different behaviors to each other, which might be related to the different basal levels of several biomarkers. Furthermore, by phenomics and genomic-based clustering, the differences between these medaka fishes were further investigated. Here, the phenomic-based clustering was based on the behavior results, while the genomic-based clustering was based on the sequence of the nd2 gene. As we expected, both clusterings showed some resemblances to each other in terms of the interspecies relationship between medaka and zebrafish. However, this similarity was not displayed by both clusterings in the medaka interspecies comparisons. Therefore, these results suggest a re-interpretation of several prior studies in comparative biology. We hope that these results contribute to the growing database of medaka fish phenotypes and provide one of the foundations for future phenomics studies of medaka fish.
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spelling pubmed-81979232021-06-14 Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics Audira, Gilbert Siregar, Petrus Chen, Kelvin H.-C. Roldan, Marri Jmelou M. Huang, Jong-Chin Lai, Hong-Thih Hsiao, Chung-Der Int J Mol Sci Article Recently, medaka has been used as a model organism in various research fields. However, even though it possesses several advantages over zebrafish, fewer studies were done in medaka compared to zebrafish, especially with regard to its behavior. Thus, to provide more information regarding its behavior and to demonstrate the behavioral differences between several species of medaka, we compared the behavioral performance and biomarker expression in the brain between four medaka fishes, Oryzias latipes, Oryzias dancena, Oryzias woworae, and Oryzias sinensis. We found that each medaka species explicitly exhibited different behaviors to each other, which might be related to the different basal levels of several biomarkers. Furthermore, by phenomics and genomic-based clustering, the differences between these medaka fishes were further investigated. Here, the phenomic-based clustering was based on the behavior results, while the genomic-based clustering was based on the sequence of the nd2 gene. As we expected, both clusterings showed some resemblances to each other in terms of the interspecies relationship between medaka and zebrafish. However, this similarity was not displayed by both clusterings in the medaka interspecies comparisons. Therefore, these results suggest a re-interpretation of several prior studies in comparative biology. We hope that these results contribute to the growing database of medaka fish phenotypes and provide one of the foundations for future phenomics studies of medaka fish. MDPI 2021-05-26 /pmc/articles/PMC8197923/ /pubmed/34073632 http://dx.doi.org/10.3390/ijms22115686 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Audira, Gilbert
Siregar, Petrus
Chen, Kelvin H.-C.
Roldan, Marri Jmelou M.
Huang, Jong-Chin
Lai, Hong-Thih
Hsiao, Chung-Der
Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics
title Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics
title_full Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics
title_fullStr Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics
title_full_unstemmed Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics
title_short Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics
title_sort interspecies behavioral variability of medaka fish assessed by comparative phenomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197923/
https://www.ncbi.nlm.nih.gov/pubmed/34073632
http://dx.doi.org/10.3390/ijms22115686
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