Cargando…

Phytochemical Fingerprinting and In Vitro Antimicrobial and Antioxidant Activity of the Aerial Parts of Thymus marschallianus Willd. and Thymus seravschanicus Klokov Growing Widely in Southern Kazakhstan

The chemical composition of the hydroethanolic extracts (60% v/v) from the aerial parts of Thymus marschallianus Willd (TM) and Thymus seravschanicus Klokov (TS) from Southern Kazakhstan flora was analyzed together with their hexane fractions. Determination of antibacterial, antifungal and antioxida...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhumakanova, Bagda Sagynaikyzy, Korona-Głowniak, Izabela, Skalicka-Woźniak, Krystyna, Ludwiczuk, Agnieszka, Baj, Tomasz, Wojtanowski, Krzysztof Kamil, Józefczyk, Aleksandra, Zhaparkulova, Karlygash Altynbekovna, Sakipova, Zuriyadda Bektemirova, Malm, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198081/
https://www.ncbi.nlm.nih.gov/pubmed/34073499
http://dx.doi.org/10.3390/molecules26113193
_version_ 1783707052752764928
author Zhumakanova, Bagda Sagynaikyzy
Korona-Głowniak, Izabela
Skalicka-Woźniak, Krystyna
Ludwiczuk, Agnieszka
Baj, Tomasz
Wojtanowski, Krzysztof Kamil
Józefczyk, Aleksandra
Zhaparkulova, Karlygash Altynbekovna
Sakipova, Zuriyadda Bektemirova
Malm, Anna
author_facet Zhumakanova, Bagda Sagynaikyzy
Korona-Głowniak, Izabela
Skalicka-Woźniak, Krystyna
Ludwiczuk, Agnieszka
Baj, Tomasz
Wojtanowski, Krzysztof Kamil
Józefczyk, Aleksandra
Zhaparkulova, Karlygash Altynbekovna
Sakipova, Zuriyadda Bektemirova
Malm, Anna
author_sort Zhumakanova, Bagda Sagynaikyzy
collection PubMed
description The chemical composition of the hydroethanolic extracts (60% v/v) from the aerial parts of Thymus marschallianus Willd (TM) and Thymus seravschanicus Klokov (TS) from Southern Kazakhstan flora was analyzed together with their hexane fractions. Determination of antibacterial, antifungal and antioxidant activities of both extracts was also performed. RP-HPLC/PDA and HPLC/ESI-QTOF-MS showed that there were some differences between the composition of both extracts. The most characteristic components of TM were rosmarinic acid, protocatechuic acid, luteolin 7-O-glucoside, and apigenin 7-O-glucuronide, while protocatechuic acid, luteolin 7-O-glucoside, luteolin 7-O-glucuronide, and eriodictyol predominated in TS. The content of polyplenols was higher in TS than in TM. The GC-MS analysis of the volatile fraction of both examined extracts revealed the presence of thymol and carvacrol. Additionally, sesquiterpenoids, fatty acids, and their ethyl esters were found in TM, and fatty acid methyl esters in TS. The antioxidant activity of both extracts was similar. The antibacterial activity of TS extract was somewhat higher than TM, while antifungal activity was the same. TS extract was the most active against Helicobacter pylori ATCC 43504 with MIC (minimal inhibitory concentration) = 0.625 mg/mL, exerting a bactericidal effect. The obtained data provide novel information about the phytochemistry of both thyme species and suggest new potential application of TS as a source of bioactive compounds, especially with anti-H. pylori activity.
format Online
Article
Text
id pubmed-8198081
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81980812021-06-14 Phytochemical Fingerprinting and In Vitro Antimicrobial and Antioxidant Activity of the Aerial Parts of Thymus marschallianus Willd. and Thymus seravschanicus Klokov Growing Widely in Southern Kazakhstan Zhumakanova, Bagda Sagynaikyzy Korona-Głowniak, Izabela Skalicka-Woźniak, Krystyna Ludwiczuk, Agnieszka Baj, Tomasz Wojtanowski, Krzysztof Kamil Józefczyk, Aleksandra Zhaparkulova, Karlygash Altynbekovna Sakipova, Zuriyadda Bektemirova Malm, Anna Molecules Article The chemical composition of the hydroethanolic extracts (60% v/v) from the aerial parts of Thymus marschallianus Willd (TM) and Thymus seravschanicus Klokov (TS) from Southern Kazakhstan flora was analyzed together with their hexane fractions. Determination of antibacterial, antifungal and antioxidant activities of both extracts was also performed. RP-HPLC/PDA and HPLC/ESI-QTOF-MS showed that there were some differences between the composition of both extracts. The most characteristic components of TM were rosmarinic acid, protocatechuic acid, luteolin 7-O-glucoside, and apigenin 7-O-glucuronide, while protocatechuic acid, luteolin 7-O-glucoside, luteolin 7-O-glucuronide, and eriodictyol predominated in TS. The content of polyplenols was higher in TS than in TM. The GC-MS analysis of the volatile fraction of both examined extracts revealed the presence of thymol and carvacrol. Additionally, sesquiterpenoids, fatty acids, and their ethyl esters were found in TM, and fatty acid methyl esters in TS. The antioxidant activity of both extracts was similar. The antibacterial activity of TS extract was somewhat higher than TM, while antifungal activity was the same. TS extract was the most active against Helicobacter pylori ATCC 43504 with MIC (minimal inhibitory concentration) = 0.625 mg/mL, exerting a bactericidal effect. The obtained data provide novel information about the phytochemistry of both thyme species and suggest new potential application of TS as a source of bioactive compounds, especially with anti-H. pylori activity. MDPI 2021-05-26 /pmc/articles/PMC8198081/ /pubmed/34073499 http://dx.doi.org/10.3390/molecules26113193 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhumakanova, Bagda Sagynaikyzy
Korona-Głowniak, Izabela
Skalicka-Woźniak, Krystyna
Ludwiczuk, Agnieszka
Baj, Tomasz
Wojtanowski, Krzysztof Kamil
Józefczyk, Aleksandra
Zhaparkulova, Karlygash Altynbekovna
Sakipova, Zuriyadda Bektemirova
Malm, Anna
Phytochemical Fingerprinting and In Vitro Antimicrobial and Antioxidant Activity of the Aerial Parts of Thymus marschallianus Willd. and Thymus seravschanicus Klokov Growing Widely in Southern Kazakhstan
title Phytochemical Fingerprinting and In Vitro Antimicrobial and Antioxidant Activity of the Aerial Parts of Thymus marschallianus Willd. and Thymus seravschanicus Klokov Growing Widely in Southern Kazakhstan
title_full Phytochemical Fingerprinting and In Vitro Antimicrobial and Antioxidant Activity of the Aerial Parts of Thymus marschallianus Willd. and Thymus seravschanicus Klokov Growing Widely in Southern Kazakhstan
title_fullStr Phytochemical Fingerprinting and In Vitro Antimicrobial and Antioxidant Activity of the Aerial Parts of Thymus marschallianus Willd. and Thymus seravschanicus Klokov Growing Widely in Southern Kazakhstan
title_full_unstemmed Phytochemical Fingerprinting and In Vitro Antimicrobial and Antioxidant Activity of the Aerial Parts of Thymus marschallianus Willd. and Thymus seravschanicus Klokov Growing Widely in Southern Kazakhstan
title_short Phytochemical Fingerprinting and In Vitro Antimicrobial and Antioxidant Activity of the Aerial Parts of Thymus marschallianus Willd. and Thymus seravschanicus Klokov Growing Widely in Southern Kazakhstan
title_sort phytochemical fingerprinting and in vitro antimicrobial and antioxidant activity of the aerial parts of thymus marschallianus willd. and thymus seravschanicus klokov growing widely in southern kazakhstan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198081/
https://www.ncbi.nlm.nih.gov/pubmed/34073499
http://dx.doi.org/10.3390/molecules26113193
work_keys_str_mv AT zhumakanovabagdasagynaikyzy phytochemicalfingerprintingandinvitroantimicrobialandantioxidantactivityoftheaerialpartsofthymusmarschallianuswilldandthymusseravschanicusklokovgrowingwidelyinsouthernkazakhstan
AT koronagłowniakizabela phytochemicalfingerprintingandinvitroantimicrobialandantioxidantactivityoftheaerialpartsofthymusmarschallianuswilldandthymusseravschanicusklokovgrowingwidelyinsouthernkazakhstan
AT skalickawozniakkrystyna phytochemicalfingerprintingandinvitroantimicrobialandantioxidantactivityoftheaerialpartsofthymusmarschallianuswilldandthymusseravschanicusklokovgrowingwidelyinsouthernkazakhstan
AT ludwiczukagnieszka phytochemicalfingerprintingandinvitroantimicrobialandantioxidantactivityoftheaerialpartsofthymusmarschallianuswilldandthymusseravschanicusklokovgrowingwidelyinsouthernkazakhstan
AT bajtomasz phytochemicalfingerprintingandinvitroantimicrobialandantioxidantactivityoftheaerialpartsofthymusmarschallianuswilldandthymusseravschanicusklokovgrowingwidelyinsouthernkazakhstan
AT wojtanowskikrzysztofkamil phytochemicalfingerprintingandinvitroantimicrobialandantioxidantactivityoftheaerialpartsofthymusmarschallianuswilldandthymusseravschanicusklokovgrowingwidelyinsouthernkazakhstan
AT jozefczykaleksandra phytochemicalfingerprintingandinvitroantimicrobialandantioxidantactivityoftheaerialpartsofthymusmarschallianuswilldandthymusseravschanicusklokovgrowingwidelyinsouthernkazakhstan
AT zhaparkulovakarlygashaltynbekovna phytochemicalfingerprintingandinvitroantimicrobialandantioxidantactivityoftheaerialpartsofthymusmarschallianuswilldandthymusseravschanicusklokovgrowingwidelyinsouthernkazakhstan
AT sakipovazuriyaddabektemirova phytochemicalfingerprintingandinvitroantimicrobialandantioxidantactivityoftheaerialpartsofthymusmarschallianuswilldandthymusseravschanicusklokovgrowingwidelyinsouthernkazakhstan
AT malmanna phytochemicalfingerprintingandinvitroantimicrobialandantioxidantactivityoftheaerialpartsofthymusmarschallianuswilldandthymusseravschanicusklokovgrowingwidelyinsouthernkazakhstan