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Synthesis and Evaluation of AlgNa-g-Poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone

Hydrogels obtained from combining different polymers are an interesting strategy for developing controlled release system platforms and tissue engineering scaffolds. In this study, the applicability of sodium alginate-g-(QCL-co-HEMA) hydrogels for these biomedical applications was evaluated. Hydroge...

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Autores principales: García-Couce, Jomarien, Vernhes, Marioly, Bada, Nancy, Agüero, Lissette, Valdés, Oscar, Alvarez-Barreto, José, Fuentes, Gastón, Almirall, Amisel, Cruz, Luis J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198102/
https://www.ncbi.nlm.nih.gov/pubmed/34072090
http://dx.doi.org/10.3390/ijms22115730
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author García-Couce, Jomarien
Vernhes, Marioly
Bada, Nancy
Agüero, Lissette
Valdés, Oscar
Alvarez-Barreto, José
Fuentes, Gastón
Almirall, Amisel
Cruz, Luis J.
author_facet García-Couce, Jomarien
Vernhes, Marioly
Bada, Nancy
Agüero, Lissette
Valdés, Oscar
Alvarez-Barreto, José
Fuentes, Gastón
Almirall, Amisel
Cruz, Luis J.
author_sort García-Couce, Jomarien
collection PubMed
description Hydrogels obtained from combining different polymers are an interesting strategy for developing controlled release system platforms and tissue engineering scaffolds. In this study, the applicability of sodium alginate-g-(QCL-co-HEMA) hydrogels for these biomedical applications was evaluated. Hydrogels were synthesized by free-radical polymerization using a different concentration of the components. The hydrogels were characterized by Fourier transform-infrared spectroscopy, scanning electron microscopy, and a swelling degree. Betamethasone release as well as the in vitro cytocompatibility with chondrocytes and fibroblast cells were also evaluated. Scanning electron microscopy confirmed the porous surface morphology of the hydrogels in all cases. The swelling percent was determined at a different pH and was observed to be pH-sensitive. The controlled release behavior of betamethasone from the matrices was investigated in PBS media (pH = 7.4) and the drug was released in a controlled manner for up to 8 h. Human chondrocytes and fibroblasts were cultured on the hydrogels. The MTS assay showed that almost all hydrogels are cytocompatibles and an increase of proliferation in both cell types after one week of incubation was observed by the Live/Dead(®) assay. These results demonstrate that these hydrogels are attractive materials for pharmaceutical and biomedical applications due to their characteristics, their release kinetics, and biocompatibility.
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spelling pubmed-81981022021-06-14 Synthesis and Evaluation of AlgNa-g-Poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone García-Couce, Jomarien Vernhes, Marioly Bada, Nancy Agüero, Lissette Valdés, Oscar Alvarez-Barreto, José Fuentes, Gastón Almirall, Amisel Cruz, Luis J. Int J Mol Sci Article Hydrogels obtained from combining different polymers are an interesting strategy for developing controlled release system platforms and tissue engineering scaffolds. In this study, the applicability of sodium alginate-g-(QCL-co-HEMA) hydrogels for these biomedical applications was evaluated. Hydrogels were synthesized by free-radical polymerization using a different concentration of the components. The hydrogels were characterized by Fourier transform-infrared spectroscopy, scanning electron microscopy, and a swelling degree. Betamethasone release as well as the in vitro cytocompatibility with chondrocytes and fibroblast cells were also evaluated. Scanning electron microscopy confirmed the porous surface morphology of the hydrogels in all cases. The swelling percent was determined at a different pH and was observed to be pH-sensitive. The controlled release behavior of betamethasone from the matrices was investigated in PBS media (pH = 7.4) and the drug was released in a controlled manner for up to 8 h. Human chondrocytes and fibroblasts were cultured on the hydrogels. The MTS assay showed that almost all hydrogels are cytocompatibles and an increase of proliferation in both cell types after one week of incubation was observed by the Live/Dead(®) assay. These results demonstrate that these hydrogels are attractive materials for pharmaceutical and biomedical applications due to their characteristics, their release kinetics, and biocompatibility. MDPI 2021-05-27 /pmc/articles/PMC8198102/ /pubmed/34072090 http://dx.doi.org/10.3390/ijms22115730 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
García-Couce, Jomarien
Vernhes, Marioly
Bada, Nancy
Agüero, Lissette
Valdés, Oscar
Alvarez-Barreto, José
Fuentes, Gastón
Almirall, Amisel
Cruz, Luis J.
Synthesis and Evaluation of AlgNa-g-Poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone
title Synthesis and Evaluation of AlgNa-g-Poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone
title_full Synthesis and Evaluation of AlgNa-g-Poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone
title_fullStr Synthesis and Evaluation of AlgNa-g-Poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone
title_full_unstemmed Synthesis and Evaluation of AlgNa-g-Poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone
title_short Synthesis and Evaluation of AlgNa-g-Poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone
title_sort synthesis and evaluation of algna-g-poly(qcl-co-hema) hydrogels as platform for chondrocyte proliferation and controlled release of betamethasone
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198102/
https://www.ncbi.nlm.nih.gov/pubmed/34072090
http://dx.doi.org/10.3390/ijms22115730
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