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Nanomechanical Hallmarks of Helicobacter pylori Infection in Pediatric Patients

Background: the molecular mechanism of gastric cancer development related to Helicobacter pylori (H. pylori) infection has not been fully understood, and further studies are still needed. Information regarding nanomechanical aspects of pathophysiological events that occur during H. pylori infection...

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Autores principales: Deptuła, Piotr, Suprewicz, Łukasz, Daniluk, Tamara, Namiot, Andrzej, Chmielewska, Sylwia Joanna, Daniluk, Urszula, Lebensztejn, Dariusz, Bucki, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198391/
https://www.ncbi.nlm.nih.gov/pubmed/34070700
http://dx.doi.org/10.3390/ijms22115624
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author Deptuła, Piotr
Suprewicz, Łukasz
Daniluk, Tamara
Namiot, Andrzej
Chmielewska, Sylwia Joanna
Daniluk, Urszula
Lebensztejn, Dariusz
Bucki, Robert
author_facet Deptuła, Piotr
Suprewicz, Łukasz
Daniluk, Tamara
Namiot, Andrzej
Chmielewska, Sylwia Joanna
Daniluk, Urszula
Lebensztejn, Dariusz
Bucki, Robert
author_sort Deptuła, Piotr
collection PubMed
description Background: the molecular mechanism of gastric cancer development related to Helicobacter pylori (H. pylori) infection has not been fully understood, and further studies are still needed. Information regarding nanomechanical aspects of pathophysiological events that occur during H. pylori infection can be crucial in the development of new prevention, treatment, and diagnostic measures against clinical consequences associated with H. pylori infection, including gastric ulcer, duodenal ulcer, and gastric cancer. Methods: in this study, we assessed mechanical properties of children’s healthy and H. pylori positive stomach tissues and the mechanical response of human gastric cells exposed to heat-treated H. pylori cells using atomic force microscopy (AFM NanoWizard 4 BioScience JPK Instruments Bruker). Elastic modulus (i.e., the Young’s modulus) was derived from the Hertz–Sneddon model applied to force-indentation curves. Human tissue samples were evaluated using rapid urease tests to identify H. pylori positive samples, and the presence of H. pylori cells in those samples was confirmed using immunohistopathological staining. Results and conclusion: collected data suggest that nanomechanical properties of infected tissue might be considered as markers indicated H. pylori presence since infected tissues are softer than uninfected ones. At the cellular level, this mechanical response is at least partially mediated by cell cytoskeleton remodeling indicating that gastric cells are able to tune their mechanical properties when subjected to the presence of H. pylori products. Persistent fluctuations of tissue mechanical properties in response to H. pylori infection might, in the long-term, promote induction of cancer development.
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spelling pubmed-81983912021-06-14 Nanomechanical Hallmarks of Helicobacter pylori Infection in Pediatric Patients Deptuła, Piotr Suprewicz, Łukasz Daniluk, Tamara Namiot, Andrzej Chmielewska, Sylwia Joanna Daniluk, Urszula Lebensztejn, Dariusz Bucki, Robert Int J Mol Sci Article Background: the molecular mechanism of gastric cancer development related to Helicobacter pylori (H. pylori) infection has not been fully understood, and further studies are still needed. Information regarding nanomechanical aspects of pathophysiological events that occur during H. pylori infection can be crucial in the development of new prevention, treatment, and diagnostic measures against clinical consequences associated with H. pylori infection, including gastric ulcer, duodenal ulcer, and gastric cancer. Methods: in this study, we assessed mechanical properties of children’s healthy and H. pylori positive stomach tissues and the mechanical response of human gastric cells exposed to heat-treated H. pylori cells using atomic force microscopy (AFM NanoWizard 4 BioScience JPK Instruments Bruker). Elastic modulus (i.e., the Young’s modulus) was derived from the Hertz–Sneddon model applied to force-indentation curves. Human tissue samples were evaluated using rapid urease tests to identify H. pylori positive samples, and the presence of H. pylori cells in those samples was confirmed using immunohistopathological staining. Results and conclusion: collected data suggest that nanomechanical properties of infected tissue might be considered as markers indicated H. pylori presence since infected tissues are softer than uninfected ones. At the cellular level, this mechanical response is at least partially mediated by cell cytoskeleton remodeling indicating that gastric cells are able to tune their mechanical properties when subjected to the presence of H. pylori products. Persistent fluctuations of tissue mechanical properties in response to H. pylori infection might, in the long-term, promote induction of cancer development. MDPI 2021-05-25 /pmc/articles/PMC8198391/ /pubmed/34070700 http://dx.doi.org/10.3390/ijms22115624 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Deptuła, Piotr
Suprewicz, Łukasz
Daniluk, Tamara
Namiot, Andrzej
Chmielewska, Sylwia Joanna
Daniluk, Urszula
Lebensztejn, Dariusz
Bucki, Robert
Nanomechanical Hallmarks of Helicobacter pylori Infection in Pediatric Patients
title Nanomechanical Hallmarks of Helicobacter pylori Infection in Pediatric Patients
title_full Nanomechanical Hallmarks of Helicobacter pylori Infection in Pediatric Patients
title_fullStr Nanomechanical Hallmarks of Helicobacter pylori Infection in Pediatric Patients
title_full_unstemmed Nanomechanical Hallmarks of Helicobacter pylori Infection in Pediatric Patients
title_short Nanomechanical Hallmarks of Helicobacter pylori Infection in Pediatric Patients
title_sort nanomechanical hallmarks of helicobacter pylori infection in pediatric patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198391/
https://www.ncbi.nlm.nih.gov/pubmed/34070700
http://dx.doi.org/10.3390/ijms22115624
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