Cargando…
The Influence of Hyaluronic Acid Biofunctionalization of a Bovine Bone Substitute on Osteoblast Activity In Vitro
Bovine bone substitute materials (BSMs) are used for oral bone regeneration. The objective was to analyze the influence of BSM biofunctionalization via hyaluronic acid (HA) on human osteoblasts (HOBs). BSMs with ± HA were incubated with HOBs including HOBs alone as a negative control. On days 3, 7 a...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198444/ https://www.ncbi.nlm.nih.gov/pubmed/34072146 http://dx.doi.org/10.3390/ma14112885 |
_version_ | 1783707140240703488 |
---|---|
author | Kyyak, Solomiya Pabst, Andreas Heimes, Diana Kämmerer, Peer W. |
author_facet | Kyyak, Solomiya Pabst, Andreas Heimes, Diana Kämmerer, Peer W. |
author_sort | Kyyak, Solomiya |
collection | PubMed |
description | Bovine bone substitute materials (BSMs) are used for oral bone regeneration. The objective was to analyze the influence of BSM biofunctionalization via hyaluronic acid (HA) on human osteoblasts (HOBs). BSMs with ± HA were incubated with HOBs including HOBs alone as a negative control. On days 3, 7 and 10, cell viability, migration and proliferation were analyzed by fluorescence staining, scratch wound assay and MTT assay. On days 3, 7 and 10, an increased cell viability was demonstrated for BSM+ compared with BSM− and the control (each p ≤ 0.05). The cell migration was enhanced for BSM+ compared with BSM− and the control after day 3 and day 7 (each p ≤ 0.05). At day 10, an accelerated wound closure was found for the control compared with BSM+/− (each p < 0.05). The highest proliferation rate was observed for BSM+ on day 3 (p ≤ 0.05) followed by BSM− and the control (each p ≤ 0.05). At day 7, a non-significantly increased proliferation was shown for BSM+ while the control was higher than BSM− (each p < 0.05). The least proliferation activity was observed for BSM− (p < 0.05) at day 10. HA biofunctionalization of the BSMs caused an increased HOB activity and might represent a promising alternative to BSM− in oral bone regeneration. |
format | Online Article Text |
id | pubmed-8198444 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81984442021-06-14 The Influence of Hyaluronic Acid Biofunctionalization of a Bovine Bone Substitute on Osteoblast Activity In Vitro Kyyak, Solomiya Pabst, Andreas Heimes, Diana Kämmerer, Peer W. Materials (Basel) Article Bovine bone substitute materials (BSMs) are used for oral bone regeneration. The objective was to analyze the influence of BSM biofunctionalization via hyaluronic acid (HA) on human osteoblasts (HOBs). BSMs with ± HA were incubated with HOBs including HOBs alone as a negative control. On days 3, 7 and 10, cell viability, migration and proliferation were analyzed by fluorescence staining, scratch wound assay and MTT assay. On days 3, 7 and 10, an increased cell viability was demonstrated for BSM+ compared with BSM− and the control (each p ≤ 0.05). The cell migration was enhanced for BSM+ compared with BSM− and the control after day 3 and day 7 (each p ≤ 0.05). At day 10, an accelerated wound closure was found for the control compared with BSM+/− (each p < 0.05). The highest proliferation rate was observed for BSM+ on day 3 (p ≤ 0.05) followed by BSM− and the control (each p ≤ 0.05). At day 7, a non-significantly increased proliferation was shown for BSM+ while the control was higher than BSM− (each p < 0.05). The least proliferation activity was observed for BSM− (p < 0.05) at day 10. HA biofunctionalization of the BSMs caused an increased HOB activity and might represent a promising alternative to BSM− in oral bone regeneration. MDPI 2021-05-27 /pmc/articles/PMC8198444/ /pubmed/34072146 http://dx.doi.org/10.3390/ma14112885 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kyyak, Solomiya Pabst, Andreas Heimes, Diana Kämmerer, Peer W. The Influence of Hyaluronic Acid Biofunctionalization of a Bovine Bone Substitute on Osteoblast Activity In Vitro |
title | The Influence of Hyaluronic Acid Biofunctionalization of a Bovine Bone Substitute on Osteoblast Activity In Vitro |
title_full | The Influence of Hyaluronic Acid Biofunctionalization of a Bovine Bone Substitute on Osteoblast Activity In Vitro |
title_fullStr | The Influence of Hyaluronic Acid Biofunctionalization of a Bovine Bone Substitute on Osteoblast Activity In Vitro |
title_full_unstemmed | The Influence of Hyaluronic Acid Biofunctionalization of a Bovine Bone Substitute on Osteoblast Activity In Vitro |
title_short | The Influence of Hyaluronic Acid Biofunctionalization of a Bovine Bone Substitute on Osteoblast Activity In Vitro |
title_sort | influence of hyaluronic acid biofunctionalization of a bovine bone substitute on osteoblast activity in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198444/ https://www.ncbi.nlm.nih.gov/pubmed/34072146 http://dx.doi.org/10.3390/ma14112885 |
work_keys_str_mv | AT kyyaksolomiya theinfluenceofhyaluronicacidbiofunctionalizationofabovinebonesubstituteonosteoblastactivityinvitro AT pabstandreas theinfluenceofhyaluronicacidbiofunctionalizationofabovinebonesubstituteonosteoblastactivityinvitro AT heimesdiana theinfluenceofhyaluronicacidbiofunctionalizationofabovinebonesubstituteonosteoblastactivityinvitro AT kammererpeerw theinfluenceofhyaluronicacidbiofunctionalizationofabovinebonesubstituteonosteoblastactivityinvitro AT kyyaksolomiya influenceofhyaluronicacidbiofunctionalizationofabovinebonesubstituteonosteoblastactivityinvitro AT pabstandreas influenceofhyaluronicacidbiofunctionalizationofabovinebonesubstituteonosteoblastactivityinvitro AT heimesdiana influenceofhyaluronicacidbiofunctionalizationofabovinebonesubstituteonosteoblastactivityinvitro AT kammererpeerw influenceofhyaluronicacidbiofunctionalizationofabovinebonesubstituteonosteoblastactivityinvitro |