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Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications
The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198538/ https://www.ncbi.nlm.nih.gov/pubmed/34072397 http://dx.doi.org/10.3390/molecules26113284 |
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author | Zahid, Muhammad Lodhi, Maria Rehan, Zulfiqar Ahmad Tayyab, Hamna Javed, Talha Shabbir, Rubab Mukhtar, Ahmed EL Sabagh, Ayman Adamski, Robert Sakran, Mohamed I. Siuta, Dorota |
author_facet | Zahid, Muhammad Lodhi, Maria Rehan, Zulfiqar Ahmad Tayyab, Hamna Javed, Talha Shabbir, Rubab Mukhtar, Ahmed EL Sabagh, Ayman Adamski, Robert Sakran, Mohamed I. Siuta, Dorota |
author_sort | Zahid, Muhammad |
collection | PubMed |
description | The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the present study, porous hydrogels were synthesized using chitosan incorporating latex of the Calotropis procera plant by using a freeze–thaw cycle to stimulate the formation of granulation tissue and angiogenesis in wound healing applications. Structural analysis through Fourier transform infrared (FTIR) spectroscopy confirmed the interaction between chitosan and Calotropis procera. Latex extract containing hydrogel showed slightly higher absorption than the control during water absorption analysis. Thermogravimetric analysis showed high thermal stability of the 60:40 combination of chitosan (CS) and Calotropis procera as compared to all other treatments and controls. A fabricated scaffold application on a chick chorioallantoic membrane (CAM) showed that all hydrogels containing latex extract resulted in a significant formation of blood vessels and regeneration of cells. Overall, the formation of connective tissues and blood capillaries and healing magnitude decreased in ascending order of concentration of extract. |
format | Online Article Text |
id | pubmed-8198538 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81985382021-06-14 Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications Zahid, Muhammad Lodhi, Maria Rehan, Zulfiqar Ahmad Tayyab, Hamna Javed, Talha Shabbir, Rubab Mukhtar, Ahmed EL Sabagh, Ayman Adamski, Robert Sakran, Mohamed I. Siuta, Dorota Molecules Article The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the present study, porous hydrogels were synthesized using chitosan incorporating latex of the Calotropis procera plant by using a freeze–thaw cycle to stimulate the formation of granulation tissue and angiogenesis in wound healing applications. Structural analysis through Fourier transform infrared (FTIR) spectroscopy confirmed the interaction between chitosan and Calotropis procera. Latex extract containing hydrogel showed slightly higher absorption than the control during water absorption analysis. Thermogravimetric analysis showed high thermal stability of the 60:40 combination of chitosan (CS) and Calotropis procera as compared to all other treatments and controls. A fabricated scaffold application on a chick chorioallantoic membrane (CAM) showed that all hydrogels containing latex extract resulted in a significant formation of blood vessels and regeneration of cells. Overall, the formation of connective tissues and blood capillaries and healing magnitude decreased in ascending order of concentration of extract. MDPI 2021-05-29 /pmc/articles/PMC8198538/ /pubmed/34072397 http://dx.doi.org/10.3390/molecules26113284 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zahid, Muhammad Lodhi, Maria Rehan, Zulfiqar Ahmad Tayyab, Hamna Javed, Talha Shabbir, Rubab Mukhtar, Ahmed EL Sabagh, Ayman Adamski, Robert Sakran, Mohamed I. Siuta, Dorota Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications |
title | Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications |
title_full | Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications |
title_fullStr | Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications |
title_full_unstemmed | Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications |
title_short | Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications |
title_sort | sustainable development of chitosan/calotropis procera-based hydrogels to stimulate formation of granulation tissue and angiogenesis in wound healing applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198538/ https://www.ncbi.nlm.nih.gov/pubmed/34072397 http://dx.doi.org/10.3390/molecules26113284 |
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