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Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications

The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the...

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Autores principales: Zahid, Muhammad, Lodhi, Maria, Rehan, Zulfiqar Ahmad, Tayyab, Hamna, Javed, Talha, Shabbir, Rubab, Mukhtar, Ahmed, EL Sabagh, Ayman, Adamski, Robert, Sakran, Mohamed I., Siuta, Dorota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198538/
https://www.ncbi.nlm.nih.gov/pubmed/34072397
http://dx.doi.org/10.3390/molecules26113284
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author Zahid, Muhammad
Lodhi, Maria
Rehan, Zulfiqar Ahmad
Tayyab, Hamna
Javed, Talha
Shabbir, Rubab
Mukhtar, Ahmed
EL Sabagh, Ayman
Adamski, Robert
Sakran, Mohamed I.
Siuta, Dorota
author_facet Zahid, Muhammad
Lodhi, Maria
Rehan, Zulfiqar Ahmad
Tayyab, Hamna
Javed, Talha
Shabbir, Rubab
Mukhtar, Ahmed
EL Sabagh, Ayman
Adamski, Robert
Sakran, Mohamed I.
Siuta, Dorota
author_sort Zahid, Muhammad
collection PubMed
description The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the present study, porous hydrogels were synthesized using chitosan incorporating latex of the Calotropis procera plant by using a freeze–thaw cycle to stimulate the formation of granulation tissue and angiogenesis in wound healing applications. Structural analysis through Fourier transform infrared (FTIR) spectroscopy confirmed the interaction between chitosan and Calotropis procera. Latex extract containing hydrogel showed slightly higher absorption than the control during water absorption analysis. Thermogravimetric analysis showed high thermal stability of the 60:40 combination of chitosan (CS) and Calotropis procera as compared to all other treatments and controls. A fabricated scaffold application on a chick chorioallantoic membrane (CAM) showed that all hydrogels containing latex extract resulted in a significant formation of blood vessels and regeneration of cells. Overall, the formation of connective tissues and blood capillaries and healing magnitude decreased in ascending order of concentration of extract.
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spelling pubmed-81985382021-06-14 Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications Zahid, Muhammad Lodhi, Maria Rehan, Zulfiqar Ahmad Tayyab, Hamna Javed, Talha Shabbir, Rubab Mukhtar, Ahmed EL Sabagh, Ayman Adamski, Robert Sakran, Mohamed I. Siuta, Dorota Molecules Article The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the present study, porous hydrogels were synthesized using chitosan incorporating latex of the Calotropis procera plant by using a freeze–thaw cycle to stimulate the formation of granulation tissue and angiogenesis in wound healing applications. Structural analysis through Fourier transform infrared (FTIR) spectroscopy confirmed the interaction between chitosan and Calotropis procera. Latex extract containing hydrogel showed slightly higher absorption than the control during water absorption analysis. Thermogravimetric analysis showed high thermal stability of the 60:40 combination of chitosan (CS) and Calotropis procera as compared to all other treatments and controls. A fabricated scaffold application on a chick chorioallantoic membrane (CAM) showed that all hydrogels containing latex extract resulted in a significant formation of blood vessels and regeneration of cells. Overall, the formation of connective tissues and blood capillaries and healing magnitude decreased in ascending order of concentration of extract. MDPI 2021-05-29 /pmc/articles/PMC8198538/ /pubmed/34072397 http://dx.doi.org/10.3390/molecules26113284 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zahid, Muhammad
Lodhi, Maria
Rehan, Zulfiqar Ahmad
Tayyab, Hamna
Javed, Talha
Shabbir, Rubab
Mukhtar, Ahmed
EL Sabagh, Ayman
Adamski, Robert
Sakran, Mohamed I.
Siuta, Dorota
Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications
title Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications
title_full Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications
title_fullStr Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications
title_full_unstemmed Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications
title_short Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications
title_sort sustainable development of chitosan/calotropis procera-based hydrogels to stimulate formation of granulation tissue and angiogenesis in wound healing applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198538/
https://www.ncbi.nlm.nih.gov/pubmed/34072397
http://dx.doi.org/10.3390/molecules26113284
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