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PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice

Immature neurons are maintained in cortical regions of the adult mammalian brain. In rodents, many of these immature neurons can be identified in the piriform cortex based on their high expression of early neuronal markers, such as doublecortin (DCX) and the polysialylated form of the neural cell ad...

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Autores principales: Coviello, Simona, Benedetti, Bruno, Jakubecova, Dominika, Belles, Maria, Klimczak, Patrycja, Gramuntell, Yaiza, Couillard-Despres, Sebastien, Nacher, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198564/
https://www.ncbi.nlm.nih.gov/pubmed/34072166
http://dx.doi.org/10.3390/ijms22115733
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author Coviello, Simona
Benedetti, Bruno
Jakubecova, Dominika
Belles, Maria
Klimczak, Patrycja
Gramuntell, Yaiza
Couillard-Despres, Sebastien
Nacher, Juan
author_facet Coviello, Simona
Benedetti, Bruno
Jakubecova, Dominika
Belles, Maria
Klimczak, Patrycja
Gramuntell, Yaiza
Couillard-Despres, Sebastien
Nacher, Juan
author_sort Coviello, Simona
collection PubMed
description Immature neurons are maintained in cortical regions of the adult mammalian brain. In rodents, many of these immature neurons can be identified in the piriform cortex based on their high expression of early neuronal markers, such as doublecortin (DCX) and the polysialylated form of the neural cell adhesion molecule (PSA-NCAM). This molecule plays critical roles in different neurodevelopmental events. Taking advantage of a DCX-CreERT2/Flox-EGFP reporter mice, we investigated the impact of targeted PSA enzymatic depletion in the piriform cortex on the fate of immature neurons. We report here that the removal of PSA accelerated the final development of immature neurons. This was revealed by a higher frequency of NeuN expression, an increase in the number of cells carrying an axon initial segment (AIS), and an increase in the number of dendrites and dendritic spines on the immature neurons. Taken together, our results demonstrated the crucial role of the PSA moiety in the protracted development of immature neurons residing outside of the neurogenic niches. More studies will be required to understand the intrinsic and extrinsic factors affecting PSA-NCAM expression to understand how the brain regulates the incorporation of these immature neurons to the established neuronal circuits of the adult brain.
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spelling pubmed-81985642021-06-14 PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice Coviello, Simona Benedetti, Bruno Jakubecova, Dominika Belles, Maria Klimczak, Patrycja Gramuntell, Yaiza Couillard-Despres, Sebastien Nacher, Juan Int J Mol Sci Article Immature neurons are maintained in cortical regions of the adult mammalian brain. In rodents, many of these immature neurons can be identified in the piriform cortex based on their high expression of early neuronal markers, such as doublecortin (DCX) and the polysialylated form of the neural cell adhesion molecule (PSA-NCAM). This molecule plays critical roles in different neurodevelopmental events. Taking advantage of a DCX-CreERT2/Flox-EGFP reporter mice, we investigated the impact of targeted PSA enzymatic depletion in the piriform cortex on the fate of immature neurons. We report here that the removal of PSA accelerated the final development of immature neurons. This was revealed by a higher frequency of NeuN expression, an increase in the number of cells carrying an axon initial segment (AIS), and an increase in the number of dendrites and dendritic spines on the immature neurons. Taken together, our results demonstrated the crucial role of the PSA moiety in the protracted development of immature neurons residing outside of the neurogenic niches. More studies will be required to understand the intrinsic and extrinsic factors affecting PSA-NCAM expression to understand how the brain regulates the incorporation of these immature neurons to the established neuronal circuits of the adult brain. MDPI 2021-05-27 /pmc/articles/PMC8198564/ /pubmed/34072166 http://dx.doi.org/10.3390/ijms22115733 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Coviello, Simona
Benedetti, Bruno
Jakubecova, Dominika
Belles, Maria
Klimczak, Patrycja
Gramuntell, Yaiza
Couillard-Despres, Sebastien
Nacher, Juan
PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice
title PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice
title_full PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice
title_fullStr PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice
title_full_unstemmed PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice
title_short PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice
title_sort psa depletion induces the differentiation of immature neurons in the piriform cortex of adult mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198564/
https://www.ncbi.nlm.nih.gov/pubmed/34072166
http://dx.doi.org/10.3390/ijms22115733
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