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Bio-Functionalized Chitosan for Bone Tissue Engineering

Hybrid biomaterials allow for the improvement of the biological properties of materials and have been successfully used for implantology in medical applications. The covalent and selective functionalization of materials with bioactive peptides provides favorable results in tissue engineering by supp...

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Detalles Bibliográficos
Autores principales: Brun, Paola, Zamuner, Annj, Battocchio, Chiara, Cassari, Leonardo, Todesco, Martina, Graziani, Valerio, Iucci, Giovanna, Marsotto, Martina, Tortora, Luca, Secchi, Valeria, Dettin, Monica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198664/
https://www.ncbi.nlm.nih.gov/pubmed/34072888
http://dx.doi.org/10.3390/ijms22115916
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author Brun, Paola
Zamuner, Annj
Battocchio, Chiara
Cassari, Leonardo
Todesco, Martina
Graziani, Valerio
Iucci, Giovanna
Marsotto, Martina
Tortora, Luca
Secchi, Valeria
Dettin, Monica
author_facet Brun, Paola
Zamuner, Annj
Battocchio, Chiara
Cassari, Leonardo
Todesco, Martina
Graziani, Valerio
Iucci, Giovanna
Marsotto, Martina
Tortora, Luca
Secchi, Valeria
Dettin, Monica
author_sort Brun, Paola
collection PubMed
description Hybrid biomaterials allow for the improvement of the biological properties of materials and have been successfully used for implantology in medical applications. The covalent and selective functionalization of materials with bioactive peptides provides favorable results in tissue engineering by supporting cell attachment to the biomaterial through biochemical cues and interaction with membrane receptors. Since the functionalization with bioactive peptides may alter the chemical and physical properties of the biomaterials, in this study we characterized the biological responses of differently functionalized chitosan analogs. Chitosan analogs were produced through the reaction of GRGDSPK (RGD) or FRHRNRKGY (HVP) sequences, both carrying an aldehyde-terminal group, to chitosan. The bio-functionalized polysaccharides, pure or “diluted” with chitosan, were chemically characterized in depth and evaluated for their antimicrobial activities and biocompatibility toward human primary osteoblast cells. The results obtained indicate that the bio-functionalization of chitosan increases human-osteoblast adhesion (p < 0.005) and proliferation (p < 0.005) as compared with chitosan. Overall, the 1:1 mixture of HVP functionalized-chitosan:chitosan is the best compromise between preserving the antibacterial properties of the material and supporting osteoblast differentiation and calcium deposition (p < 0.005 vs. RGD). In conclusion, our results reported that a selected concentration of HVP supported the biomimetic potential of functionalized chitosan better than RGD and preserved the antibacterial properties of chitosan.
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spelling pubmed-81986642021-06-14 Bio-Functionalized Chitosan for Bone Tissue Engineering Brun, Paola Zamuner, Annj Battocchio, Chiara Cassari, Leonardo Todesco, Martina Graziani, Valerio Iucci, Giovanna Marsotto, Martina Tortora, Luca Secchi, Valeria Dettin, Monica Int J Mol Sci Article Hybrid biomaterials allow for the improvement of the biological properties of materials and have been successfully used for implantology in medical applications. The covalent and selective functionalization of materials with bioactive peptides provides favorable results in tissue engineering by supporting cell attachment to the biomaterial through biochemical cues and interaction with membrane receptors. Since the functionalization with bioactive peptides may alter the chemical and physical properties of the biomaterials, in this study we characterized the biological responses of differently functionalized chitosan analogs. Chitosan analogs were produced through the reaction of GRGDSPK (RGD) or FRHRNRKGY (HVP) sequences, both carrying an aldehyde-terminal group, to chitosan. The bio-functionalized polysaccharides, pure or “diluted” with chitosan, were chemically characterized in depth and evaluated for their antimicrobial activities and biocompatibility toward human primary osteoblast cells. The results obtained indicate that the bio-functionalization of chitosan increases human-osteoblast adhesion (p < 0.005) and proliferation (p < 0.005) as compared with chitosan. Overall, the 1:1 mixture of HVP functionalized-chitosan:chitosan is the best compromise between preserving the antibacterial properties of the material and supporting osteoblast differentiation and calcium deposition (p < 0.005 vs. RGD). In conclusion, our results reported that a selected concentration of HVP supported the biomimetic potential of functionalized chitosan better than RGD and preserved the antibacterial properties of chitosan. MDPI 2021-05-31 /pmc/articles/PMC8198664/ /pubmed/34072888 http://dx.doi.org/10.3390/ijms22115916 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Brun, Paola
Zamuner, Annj
Battocchio, Chiara
Cassari, Leonardo
Todesco, Martina
Graziani, Valerio
Iucci, Giovanna
Marsotto, Martina
Tortora, Luca
Secchi, Valeria
Dettin, Monica
Bio-Functionalized Chitosan for Bone Tissue Engineering
title Bio-Functionalized Chitosan for Bone Tissue Engineering
title_full Bio-Functionalized Chitosan for Bone Tissue Engineering
title_fullStr Bio-Functionalized Chitosan for Bone Tissue Engineering
title_full_unstemmed Bio-Functionalized Chitosan for Bone Tissue Engineering
title_short Bio-Functionalized Chitosan for Bone Tissue Engineering
title_sort bio-functionalized chitosan for bone tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198664/
https://www.ncbi.nlm.nih.gov/pubmed/34072888
http://dx.doi.org/10.3390/ijms22115916
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