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Bio-Functionalized Chitosan for Bone Tissue Engineering
Hybrid biomaterials allow for the improvement of the biological properties of materials and have been successfully used for implantology in medical applications. The covalent and selective functionalization of materials with bioactive peptides provides favorable results in tissue engineering by supp...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198664/ https://www.ncbi.nlm.nih.gov/pubmed/34072888 http://dx.doi.org/10.3390/ijms22115916 |
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author | Brun, Paola Zamuner, Annj Battocchio, Chiara Cassari, Leonardo Todesco, Martina Graziani, Valerio Iucci, Giovanna Marsotto, Martina Tortora, Luca Secchi, Valeria Dettin, Monica |
author_facet | Brun, Paola Zamuner, Annj Battocchio, Chiara Cassari, Leonardo Todesco, Martina Graziani, Valerio Iucci, Giovanna Marsotto, Martina Tortora, Luca Secchi, Valeria Dettin, Monica |
author_sort | Brun, Paola |
collection | PubMed |
description | Hybrid biomaterials allow for the improvement of the biological properties of materials and have been successfully used for implantology in medical applications. The covalent and selective functionalization of materials with bioactive peptides provides favorable results in tissue engineering by supporting cell attachment to the biomaterial through biochemical cues and interaction with membrane receptors. Since the functionalization with bioactive peptides may alter the chemical and physical properties of the biomaterials, in this study we characterized the biological responses of differently functionalized chitosan analogs. Chitosan analogs were produced through the reaction of GRGDSPK (RGD) or FRHRNRKGY (HVP) sequences, both carrying an aldehyde-terminal group, to chitosan. The bio-functionalized polysaccharides, pure or “diluted” with chitosan, were chemically characterized in depth and evaluated for their antimicrobial activities and biocompatibility toward human primary osteoblast cells. The results obtained indicate that the bio-functionalization of chitosan increases human-osteoblast adhesion (p < 0.005) and proliferation (p < 0.005) as compared with chitosan. Overall, the 1:1 mixture of HVP functionalized-chitosan:chitosan is the best compromise between preserving the antibacterial properties of the material and supporting osteoblast differentiation and calcium deposition (p < 0.005 vs. RGD). In conclusion, our results reported that a selected concentration of HVP supported the biomimetic potential of functionalized chitosan better than RGD and preserved the antibacterial properties of chitosan. |
format | Online Article Text |
id | pubmed-8198664 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81986642021-06-14 Bio-Functionalized Chitosan for Bone Tissue Engineering Brun, Paola Zamuner, Annj Battocchio, Chiara Cassari, Leonardo Todesco, Martina Graziani, Valerio Iucci, Giovanna Marsotto, Martina Tortora, Luca Secchi, Valeria Dettin, Monica Int J Mol Sci Article Hybrid biomaterials allow for the improvement of the biological properties of materials and have been successfully used for implantology in medical applications. The covalent and selective functionalization of materials with bioactive peptides provides favorable results in tissue engineering by supporting cell attachment to the biomaterial through biochemical cues and interaction with membrane receptors. Since the functionalization with bioactive peptides may alter the chemical and physical properties of the biomaterials, in this study we characterized the biological responses of differently functionalized chitosan analogs. Chitosan analogs were produced through the reaction of GRGDSPK (RGD) or FRHRNRKGY (HVP) sequences, both carrying an aldehyde-terminal group, to chitosan. The bio-functionalized polysaccharides, pure or “diluted” with chitosan, were chemically characterized in depth and evaluated for their antimicrobial activities and biocompatibility toward human primary osteoblast cells. The results obtained indicate that the bio-functionalization of chitosan increases human-osteoblast adhesion (p < 0.005) and proliferation (p < 0.005) as compared with chitosan. Overall, the 1:1 mixture of HVP functionalized-chitosan:chitosan is the best compromise between preserving the antibacterial properties of the material and supporting osteoblast differentiation and calcium deposition (p < 0.005 vs. RGD). In conclusion, our results reported that a selected concentration of HVP supported the biomimetic potential of functionalized chitosan better than RGD and preserved the antibacterial properties of chitosan. MDPI 2021-05-31 /pmc/articles/PMC8198664/ /pubmed/34072888 http://dx.doi.org/10.3390/ijms22115916 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Brun, Paola Zamuner, Annj Battocchio, Chiara Cassari, Leonardo Todesco, Martina Graziani, Valerio Iucci, Giovanna Marsotto, Martina Tortora, Luca Secchi, Valeria Dettin, Monica Bio-Functionalized Chitosan for Bone Tissue Engineering |
title | Bio-Functionalized Chitosan for Bone Tissue Engineering |
title_full | Bio-Functionalized Chitosan for Bone Tissue Engineering |
title_fullStr | Bio-Functionalized Chitosan for Bone Tissue Engineering |
title_full_unstemmed | Bio-Functionalized Chitosan for Bone Tissue Engineering |
title_short | Bio-Functionalized Chitosan for Bone Tissue Engineering |
title_sort | bio-functionalized chitosan for bone tissue engineering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198664/ https://www.ncbi.nlm.nih.gov/pubmed/34072888 http://dx.doi.org/10.3390/ijms22115916 |
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