Supramolecular Chromatographic Separation of C(60) and C(70) Fullerenes: Flash Column Chromatography vs. High Pressure Liquid Chromatography

A silica-bound C-butylpyrogallol[4]arene chromatographic stationary phase was prepared and characterised by thermogravimetric analysis, scanning electron microscopy, NMR and mass spectrometry. The chromatographic performance was investigated by using C(60) and C(70) fullerenes in reverse phase mode...

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Autores principales: Mekapothula, Subbareddy, Wonanke, A. D. Dinga, Addicoat, Matthew A., Boocock, David J., Wallis, John D., Cave, Gareth W. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198677/
https://www.ncbi.nlm.nih.gov/pubmed/34072234
http://dx.doi.org/10.3390/ijms22115726
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author Mekapothula, Subbareddy
Wonanke, A. D. Dinga
Addicoat, Matthew A.
Boocock, David J.
Wallis, John D.
Cave, Gareth W. V.
author_facet Mekapothula, Subbareddy
Wonanke, A. D. Dinga
Addicoat, Matthew A.
Boocock, David J.
Wallis, John D.
Cave, Gareth W. V.
author_sort Mekapothula, Subbareddy
collection PubMed
description A silica-bound C-butylpyrogallol[4]arene chromatographic stationary phase was prepared and characterised by thermogravimetric analysis, scanning electron microscopy, NMR and mass spectrometry. The chromatographic performance was investigated by using C(60) and C(70) fullerenes in reverse phase mode via flash column and high-pressure liquid chromatography (HPLC). The resulting new stationary phase was observed to demonstrate size-selective molecular recognition as postulated from our in-silico studies. The silica-bound C-butylpyrogallol[4]arene flash and HPLC stationary phases were able to separate a C(60)- and C(70)-fullerene mixture more effectively than an RP-C(18) stationary phase. The presence of toluene in the mobile phase plays a significant role in achieving symmetrical peaks in flash column chromatography.
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spelling pubmed-81986772021-06-14 Supramolecular Chromatographic Separation of C(60) and C(70) Fullerenes: Flash Column Chromatography vs. High Pressure Liquid Chromatography Mekapothula, Subbareddy Wonanke, A. D. Dinga Addicoat, Matthew A. Boocock, David J. Wallis, John D. Cave, Gareth W. V. Int J Mol Sci Article A silica-bound C-butylpyrogallol[4]arene chromatographic stationary phase was prepared and characterised by thermogravimetric analysis, scanning electron microscopy, NMR and mass spectrometry. The chromatographic performance was investigated by using C(60) and C(70) fullerenes in reverse phase mode via flash column and high-pressure liquid chromatography (HPLC). The resulting new stationary phase was observed to demonstrate size-selective molecular recognition as postulated from our in-silico studies. The silica-bound C-butylpyrogallol[4]arene flash and HPLC stationary phases were able to separate a C(60)- and C(70)-fullerene mixture more effectively than an RP-C(18) stationary phase. The presence of toluene in the mobile phase plays a significant role in achieving symmetrical peaks in flash column chromatography. MDPI 2021-05-27 /pmc/articles/PMC8198677/ /pubmed/34072234 http://dx.doi.org/10.3390/ijms22115726 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mekapothula, Subbareddy
Wonanke, A. D. Dinga
Addicoat, Matthew A.
Boocock, David J.
Wallis, John D.
Cave, Gareth W. V.
Supramolecular Chromatographic Separation of C(60) and C(70) Fullerenes: Flash Column Chromatography vs. High Pressure Liquid Chromatography
title Supramolecular Chromatographic Separation of C(60) and C(70) Fullerenes: Flash Column Chromatography vs. High Pressure Liquid Chromatography
title_full Supramolecular Chromatographic Separation of C(60) and C(70) Fullerenes: Flash Column Chromatography vs. High Pressure Liquid Chromatography
title_fullStr Supramolecular Chromatographic Separation of C(60) and C(70) Fullerenes: Flash Column Chromatography vs. High Pressure Liquid Chromatography
title_full_unstemmed Supramolecular Chromatographic Separation of C(60) and C(70) Fullerenes: Flash Column Chromatography vs. High Pressure Liquid Chromatography
title_short Supramolecular Chromatographic Separation of C(60) and C(70) Fullerenes: Flash Column Chromatography vs. High Pressure Liquid Chromatography
title_sort supramolecular chromatographic separation of c(60) and c(70) fullerenes: flash column chromatography vs. high pressure liquid chromatography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198677/
https://www.ncbi.nlm.nih.gov/pubmed/34072234
http://dx.doi.org/10.3390/ijms22115726
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