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A Matrix Metalloproteinase Mediates Tracheal Development in Bombyx mori

The trachea of insects is a tubular epithelia tissue that transports oxygen and other gases. It serves as a useful model for the studying of the cellular and molecular events involved in epithelial tube formation. Almost all of the extracellular matrix can be degraded by Matrix metalloproteinases (M...

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Autores principales: Wei, Yi, Zhou, Xiao-Lin, Liu, Tai-Hang, Chen, Peng, Jiang, Xia, Dong, Zhan-Qi, Pan, Min-Hui, Lu, Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198827/
https://www.ncbi.nlm.nih.gov/pubmed/34070691
http://dx.doi.org/10.3390/ijms22115618
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author Wei, Yi
Zhou, Xiao-Lin
Liu, Tai-Hang
Chen, Peng
Jiang, Xia
Dong, Zhan-Qi
Pan, Min-Hui
Lu, Cheng
author_facet Wei, Yi
Zhou, Xiao-Lin
Liu, Tai-Hang
Chen, Peng
Jiang, Xia
Dong, Zhan-Qi
Pan, Min-Hui
Lu, Cheng
author_sort Wei, Yi
collection PubMed
description The trachea of insects is a tubular epithelia tissue that transports oxygen and other gases. It serves as a useful model for the studying of the cellular and molecular events involved in epithelial tube formation. Almost all of the extracellular matrix can be degraded by Matrix metalloproteinases (MMPs), which is closely related to the processes of development and regeneration. The regulation of trachea by MMPs is roughly known in previous studies, but the detailed regulation mechanism and involved gene function are not fully explored. In this article, we found MMP1 expressed highly during tracheal remodeling, and knocked out it makes the tracheal branch number reduced in Bombyx mori. In trachea of transgenic BmMMP1-KO silkworm, the space expanding of taenidium and epidermal cells and the structure of apical membrane were abnormal. To explore the underlying mechanism, we detected that DE-cadherin and Integrin β1 were accumulated in trachea of transgenic BmMMP1-KO silkworm by immunohistochemistry. Moreover, 5-Bromo-2′-Deoxyuridine (BrdU) labeling showed that knockout of BmMMP1 in silkworm inhibited tracheal cell proliferation, and BmMMP1 also regulated the proliferation and migration of BmNS cells. All of the results demonstrated that BmMMP1 regulates the development of the tracheal tissue by expanding the space of tracheal cuticles and increases the number of tracheal branches by degrading DE-cadherin and Integrin β1.
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spelling pubmed-81988272021-06-14 A Matrix Metalloproteinase Mediates Tracheal Development in Bombyx mori Wei, Yi Zhou, Xiao-Lin Liu, Tai-Hang Chen, Peng Jiang, Xia Dong, Zhan-Qi Pan, Min-Hui Lu, Cheng Int J Mol Sci Article The trachea of insects is a tubular epithelia tissue that transports oxygen and other gases. It serves as a useful model for the studying of the cellular and molecular events involved in epithelial tube formation. Almost all of the extracellular matrix can be degraded by Matrix metalloproteinases (MMPs), which is closely related to the processes of development and regeneration. The regulation of trachea by MMPs is roughly known in previous studies, but the detailed regulation mechanism and involved gene function are not fully explored. In this article, we found MMP1 expressed highly during tracheal remodeling, and knocked out it makes the tracheal branch number reduced in Bombyx mori. In trachea of transgenic BmMMP1-KO silkworm, the space expanding of taenidium and epidermal cells and the structure of apical membrane were abnormal. To explore the underlying mechanism, we detected that DE-cadherin and Integrin β1 were accumulated in trachea of transgenic BmMMP1-KO silkworm by immunohistochemistry. Moreover, 5-Bromo-2′-Deoxyuridine (BrdU) labeling showed that knockout of BmMMP1 in silkworm inhibited tracheal cell proliferation, and BmMMP1 also regulated the proliferation and migration of BmNS cells. All of the results demonstrated that BmMMP1 regulates the development of the tracheal tissue by expanding the space of tracheal cuticles and increases the number of tracheal branches by degrading DE-cadherin and Integrin β1. MDPI 2021-05-25 /pmc/articles/PMC8198827/ /pubmed/34070691 http://dx.doi.org/10.3390/ijms22115618 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wei, Yi
Zhou, Xiao-Lin
Liu, Tai-Hang
Chen, Peng
Jiang, Xia
Dong, Zhan-Qi
Pan, Min-Hui
Lu, Cheng
A Matrix Metalloproteinase Mediates Tracheal Development in Bombyx mori
title A Matrix Metalloproteinase Mediates Tracheal Development in Bombyx mori
title_full A Matrix Metalloproteinase Mediates Tracheal Development in Bombyx mori
title_fullStr A Matrix Metalloproteinase Mediates Tracheal Development in Bombyx mori
title_full_unstemmed A Matrix Metalloproteinase Mediates Tracheal Development in Bombyx mori
title_short A Matrix Metalloproteinase Mediates Tracheal Development in Bombyx mori
title_sort matrix metalloproteinase mediates tracheal development in bombyx mori
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198827/
https://www.ncbi.nlm.nih.gov/pubmed/34070691
http://dx.doi.org/10.3390/ijms22115618
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