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Random Lasing Detection of Mutant Huntingtin Expression in Cells
Huntington’s disease (HD) is an autosomal dominant, incurable neurodegenerative disease caused by mutation in the huntingtin gene (HTT). HTT mutation leads to protein misfolding and aggregation, which affect cells’ functions and structural features. Because these changes might modify the scattering...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198928/ https://www.ncbi.nlm.nih.gov/pubmed/34073127 http://dx.doi.org/10.3390/s21113825 |
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author | de Armas-Rillo, Sergio Fumagallo-Reading, Felipe Luis-Ravelo, Diego Abdul-Jalbar, Beatriz González-Hernández, Tomás Lahoz, Fernando |
author_facet | de Armas-Rillo, Sergio Fumagallo-Reading, Felipe Luis-Ravelo, Diego Abdul-Jalbar, Beatriz González-Hernández, Tomás Lahoz, Fernando |
author_sort | de Armas-Rillo, Sergio |
collection | PubMed |
description | Huntington’s disease (HD) is an autosomal dominant, incurable neurodegenerative disease caused by mutation in the huntingtin gene (HTT). HTT mutation leads to protein misfolding and aggregation, which affect cells’ functions and structural features. Because these changes might modify the scattering strength of affected cells, we propose that random lasing (RL) is an appropriate technique for detecting cells that express mutated HTT. To explore this hypothesis, we used a cell model of HD based on the expression of two different forms—pathogenic and non-pathogenic—of HTT. The RL signals from both cell profiles were compared. A multivariate statistical analysis of the RL signals based on the principal component analysis (PCA) and linear discriminant analysis (LDA) techniques revealed substantial differences between cells that expressed the pathogenic and the non-pathogenic forms of HTT. |
format | Online Article Text |
id | pubmed-8198928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81989282021-06-14 Random Lasing Detection of Mutant Huntingtin Expression in Cells de Armas-Rillo, Sergio Fumagallo-Reading, Felipe Luis-Ravelo, Diego Abdul-Jalbar, Beatriz González-Hernández, Tomás Lahoz, Fernando Sensors (Basel) Article Huntington’s disease (HD) is an autosomal dominant, incurable neurodegenerative disease caused by mutation in the huntingtin gene (HTT). HTT mutation leads to protein misfolding and aggregation, which affect cells’ functions and structural features. Because these changes might modify the scattering strength of affected cells, we propose that random lasing (RL) is an appropriate technique for detecting cells that express mutated HTT. To explore this hypothesis, we used a cell model of HD based on the expression of two different forms—pathogenic and non-pathogenic—of HTT. The RL signals from both cell profiles were compared. A multivariate statistical analysis of the RL signals based on the principal component analysis (PCA) and linear discriminant analysis (LDA) techniques revealed substantial differences between cells that expressed the pathogenic and the non-pathogenic forms of HTT. MDPI 2021-05-31 /pmc/articles/PMC8198928/ /pubmed/34073127 http://dx.doi.org/10.3390/s21113825 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article de Armas-Rillo, Sergio Fumagallo-Reading, Felipe Luis-Ravelo, Diego Abdul-Jalbar, Beatriz González-Hernández, Tomás Lahoz, Fernando Random Lasing Detection of Mutant Huntingtin Expression in Cells |
title | Random Lasing Detection of Mutant Huntingtin Expression in Cells |
title_full | Random Lasing Detection of Mutant Huntingtin Expression in Cells |
title_fullStr | Random Lasing Detection of Mutant Huntingtin Expression in Cells |
title_full_unstemmed | Random Lasing Detection of Mutant Huntingtin Expression in Cells |
title_short | Random Lasing Detection of Mutant Huntingtin Expression in Cells |
title_sort | random lasing detection of mutant huntingtin expression in cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198928/ https://www.ncbi.nlm.nih.gov/pubmed/34073127 http://dx.doi.org/10.3390/s21113825 |
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