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Random Lasing Detection of Mutant Huntingtin Expression in Cells

Huntington’s disease (HD) is an autosomal dominant, incurable neurodegenerative disease caused by mutation in the huntingtin gene (HTT). HTT mutation leads to protein misfolding and aggregation, which affect cells’ functions and structural features. Because these changes might modify the scattering...

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Autores principales: de Armas-Rillo, Sergio, Fumagallo-Reading, Felipe, Luis-Ravelo, Diego, Abdul-Jalbar, Beatriz, González-Hernández, Tomás, Lahoz, Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198928/
https://www.ncbi.nlm.nih.gov/pubmed/34073127
http://dx.doi.org/10.3390/s21113825
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author de Armas-Rillo, Sergio
Fumagallo-Reading, Felipe
Luis-Ravelo, Diego
Abdul-Jalbar, Beatriz
González-Hernández, Tomás
Lahoz, Fernando
author_facet de Armas-Rillo, Sergio
Fumagallo-Reading, Felipe
Luis-Ravelo, Diego
Abdul-Jalbar, Beatriz
González-Hernández, Tomás
Lahoz, Fernando
author_sort de Armas-Rillo, Sergio
collection PubMed
description Huntington’s disease (HD) is an autosomal dominant, incurable neurodegenerative disease caused by mutation in the huntingtin gene (HTT). HTT mutation leads to protein misfolding and aggregation, which affect cells’ functions and structural features. Because these changes might modify the scattering strength of affected cells, we propose that random lasing (RL) is an appropriate technique for detecting cells that express mutated HTT. To explore this hypothesis, we used a cell model of HD based on the expression of two different forms—pathogenic and non-pathogenic—of HTT. The RL signals from both cell profiles were compared. A multivariate statistical analysis of the RL signals based on the principal component analysis (PCA) and linear discriminant analysis (LDA) techniques revealed substantial differences between cells that expressed the pathogenic and the non-pathogenic forms of HTT.
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spelling pubmed-81989282021-06-14 Random Lasing Detection of Mutant Huntingtin Expression in Cells de Armas-Rillo, Sergio Fumagallo-Reading, Felipe Luis-Ravelo, Diego Abdul-Jalbar, Beatriz González-Hernández, Tomás Lahoz, Fernando Sensors (Basel) Article Huntington’s disease (HD) is an autosomal dominant, incurable neurodegenerative disease caused by mutation in the huntingtin gene (HTT). HTT mutation leads to protein misfolding and aggregation, which affect cells’ functions and structural features. Because these changes might modify the scattering strength of affected cells, we propose that random lasing (RL) is an appropriate technique for detecting cells that express mutated HTT. To explore this hypothesis, we used a cell model of HD based on the expression of two different forms—pathogenic and non-pathogenic—of HTT. The RL signals from both cell profiles were compared. A multivariate statistical analysis of the RL signals based on the principal component analysis (PCA) and linear discriminant analysis (LDA) techniques revealed substantial differences between cells that expressed the pathogenic and the non-pathogenic forms of HTT. MDPI 2021-05-31 /pmc/articles/PMC8198928/ /pubmed/34073127 http://dx.doi.org/10.3390/s21113825 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Armas-Rillo, Sergio
Fumagallo-Reading, Felipe
Luis-Ravelo, Diego
Abdul-Jalbar, Beatriz
González-Hernández, Tomás
Lahoz, Fernando
Random Lasing Detection of Mutant Huntingtin Expression in Cells
title Random Lasing Detection of Mutant Huntingtin Expression in Cells
title_full Random Lasing Detection of Mutant Huntingtin Expression in Cells
title_fullStr Random Lasing Detection of Mutant Huntingtin Expression in Cells
title_full_unstemmed Random Lasing Detection of Mutant Huntingtin Expression in Cells
title_short Random Lasing Detection of Mutant Huntingtin Expression in Cells
title_sort random lasing detection of mutant huntingtin expression in cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198928/
https://www.ncbi.nlm.nih.gov/pubmed/34073127
http://dx.doi.org/10.3390/s21113825
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