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Can Human Oral Mucosa Stem Cells Differentiate to Corneal Epithelia?

Human oral mucosa stem cells (hOMSCs) arise from the neural crest, they can self-renew, proliferate, and differentiate to several cell lines and could represent a good source for application in tissue engineering. Because of their anatomical location, hOMSCs are easy to isolate, have multilineage di...

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Autores principales: López, Sonia, Hoz, Lía, Tenorio, Eda Patricia, Buentello, Beatriz, Magaña, Fátima Sofía, Wintergerst, Ana, Navas, Alejandro, Garfias, Yonathan, Arzate, Higinio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198937/
https://www.ncbi.nlm.nih.gov/pubmed/34205905
http://dx.doi.org/10.3390/ijms22115976
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author López, Sonia
Hoz, Lía
Tenorio, Eda Patricia
Buentello, Beatriz
Magaña, Fátima Sofía
Wintergerst, Ana
Navas, Alejandro
Garfias, Yonathan
Arzate, Higinio
author_facet López, Sonia
Hoz, Lía
Tenorio, Eda Patricia
Buentello, Beatriz
Magaña, Fátima Sofía
Wintergerst, Ana
Navas, Alejandro
Garfias, Yonathan
Arzate, Higinio
author_sort López, Sonia
collection PubMed
description Human oral mucosa stem cells (hOMSCs) arise from the neural crest, they can self-renew, proliferate, and differentiate to several cell lines and could represent a good source for application in tissue engineering. Because of their anatomical location, hOMSCs are easy to isolate, have multilineage differentiation capacity and express embryonic stem cells markers such as—Sox2, Oct3/4 and Nanog. We have used SHEM (supplemented hormonal epithelial medium) media and cultured hOMSCs over human amniotic membrane and determined the cell’s capacity to differentiate to an epithelial-like phenotype and to express corneal specific epithelial markers—CK3, CK12, CK19, Pan-cadherin and E-cadherin. Our results showed that hOMSCs possess the capacity to attach to the amniotic membrane and express CK3, CK19, Pan-Cadherin and E-Cadherin without induction with SHEM media and expressed CK12 or changed the expression pattern of E-Cadherin to a punctual-like feature when treated with SHEM media. The results observed in this study show that hOMSCs possess the potential to differentiate toward epithelial cells. In conclusion, our results revealed that hOMSCs readily express markers for corneal determination and could provide the ophthalmology field with a therapeutic alternative for tissue engineering to achieve corneal replacement when compared with other techniques. Nevertheless, further studies are needed to develop a predictable therapeutic alternative for cornea replacement.
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spelling pubmed-81989372021-06-14 Can Human Oral Mucosa Stem Cells Differentiate to Corneal Epithelia? López, Sonia Hoz, Lía Tenorio, Eda Patricia Buentello, Beatriz Magaña, Fátima Sofía Wintergerst, Ana Navas, Alejandro Garfias, Yonathan Arzate, Higinio Int J Mol Sci Article Human oral mucosa stem cells (hOMSCs) arise from the neural crest, they can self-renew, proliferate, and differentiate to several cell lines and could represent a good source for application in tissue engineering. Because of their anatomical location, hOMSCs are easy to isolate, have multilineage differentiation capacity and express embryonic stem cells markers such as—Sox2, Oct3/4 and Nanog. We have used SHEM (supplemented hormonal epithelial medium) media and cultured hOMSCs over human amniotic membrane and determined the cell’s capacity to differentiate to an epithelial-like phenotype and to express corneal specific epithelial markers—CK3, CK12, CK19, Pan-cadherin and E-cadherin. Our results showed that hOMSCs possess the capacity to attach to the amniotic membrane and express CK3, CK19, Pan-Cadherin and E-Cadherin without induction with SHEM media and expressed CK12 or changed the expression pattern of E-Cadherin to a punctual-like feature when treated with SHEM media. The results observed in this study show that hOMSCs possess the potential to differentiate toward epithelial cells. In conclusion, our results revealed that hOMSCs readily express markers for corneal determination and could provide the ophthalmology field with a therapeutic alternative for tissue engineering to achieve corneal replacement when compared with other techniques. Nevertheless, further studies are needed to develop a predictable therapeutic alternative for cornea replacement. MDPI 2021-06-01 /pmc/articles/PMC8198937/ /pubmed/34205905 http://dx.doi.org/10.3390/ijms22115976 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
López, Sonia
Hoz, Lía
Tenorio, Eda Patricia
Buentello, Beatriz
Magaña, Fátima Sofía
Wintergerst, Ana
Navas, Alejandro
Garfias, Yonathan
Arzate, Higinio
Can Human Oral Mucosa Stem Cells Differentiate to Corneal Epithelia?
title Can Human Oral Mucosa Stem Cells Differentiate to Corneal Epithelia?
title_full Can Human Oral Mucosa Stem Cells Differentiate to Corneal Epithelia?
title_fullStr Can Human Oral Mucosa Stem Cells Differentiate to Corneal Epithelia?
title_full_unstemmed Can Human Oral Mucosa Stem Cells Differentiate to Corneal Epithelia?
title_short Can Human Oral Mucosa Stem Cells Differentiate to Corneal Epithelia?
title_sort can human oral mucosa stem cells differentiate to corneal epithelia?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198937/
https://www.ncbi.nlm.nih.gov/pubmed/34205905
http://dx.doi.org/10.3390/ijms22115976
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