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Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress

Although recent studies suggest that the plant cytoskeleton is associated with plant stress responses, such as salt, cold, and drought, the molecular mechanism underlying microtubule function in plant salt stress response remains unclear. We performed a comparative proteomic analysis between control...

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Autores principales: Chun, Hyun Jin, Baek, Dongwon, Jin, Byung Jun, Cho, Hyun Min, Park, Mi Suk, Lee, Su Hyeon, Lim, Lack Hyeon, Cha, Ye Jin, Bae, Dong-Won, Kim, Sun Tae, Yun, Dae-Jin, Kim, Min Chul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199277/
https://www.ncbi.nlm.nih.gov/pubmed/34073070
http://dx.doi.org/10.3390/ijms22115957
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author Chun, Hyun Jin
Baek, Dongwon
Jin, Byung Jun
Cho, Hyun Min
Park, Mi Suk
Lee, Su Hyeon
Lim, Lack Hyeon
Cha, Ye Jin
Bae, Dong-Won
Kim, Sun Tae
Yun, Dae-Jin
Kim, Min Chul
author_facet Chun, Hyun Jin
Baek, Dongwon
Jin, Byung Jun
Cho, Hyun Min
Park, Mi Suk
Lee, Su Hyeon
Lim, Lack Hyeon
Cha, Ye Jin
Bae, Dong-Won
Kim, Sun Tae
Yun, Dae-Jin
Kim, Min Chul
author_sort Chun, Hyun Jin
collection PubMed
description Although recent studies suggest that the plant cytoskeleton is associated with plant stress responses, such as salt, cold, and drought, the molecular mechanism underlying microtubule function in plant salt stress response remains unclear. We performed a comparative proteomic analysis between control suspension-cultured cells (A0) and salt-adapted cells (A120) established from Arabidopsis root callus to investigate plant adaptation mechanisms to long-term salt stress. We identified 50 differentially expressed proteins (45 up- and 5 down-regulated proteins) in A120 cells compared with A0 cells. Gene ontology enrichment and protein network analyses indicated that differentially expressed proteins in A120 cells were strongly associated with cell structure-associated clusters, including cytoskeleton and cell wall biogenesis. Gene expression analysis revealed that expressions of cytoskeleton-related genes, such as FBA8, TUB3, TUB4, TUB7, TUB9, and ACT7, and a cell wall biogenesis-related gene, CCoAOMT1, were induced in salt-adapted A120 cells. Moreover, the loss-of-function mutant of Arabidopsis TUB9 gene, tub9, showed a hypersensitive phenotype to salt stress. Consistent overexpression of Arabidopsis TUB9 gene in rice transgenic plants enhanced tolerance to salt stress. Our results suggest that microtubules play crucial roles in plant adaptation and tolerance to salt stress. The modulation of microtubule-related gene expression can be an effective strategy for developing salt-tolerant crops.
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spelling pubmed-81992772021-06-14 Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress Chun, Hyun Jin Baek, Dongwon Jin, Byung Jun Cho, Hyun Min Park, Mi Suk Lee, Su Hyeon Lim, Lack Hyeon Cha, Ye Jin Bae, Dong-Won Kim, Sun Tae Yun, Dae-Jin Kim, Min Chul Int J Mol Sci Article Although recent studies suggest that the plant cytoskeleton is associated with plant stress responses, such as salt, cold, and drought, the molecular mechanism underlying microtubule function in plant salt stress response remains unclear. We performed a comparative proteomic analysis between control suspension-cultured cells (A0) and salt-adapted cells (A120) established from Arabidopsis root callus to investigate plant adaptation mechanisms to long-term salt stress. We identified 50 differentially expressed proteins (45 up- and 5 down-regulated proteins) in A120 cells compared with A0 cells. Gene ontology enrichment and protein network analyses indicated that differentially expressed proteins in A120 cells were strongly associated with cell structure-associated clusters, including cytoskeleton and cell wall biogenesis. Gene expression analysis revealed that expressions of cytoskeleton-related genes, such as FBA8, TUB3, TUB4, TUB7, TUB9, and ACT7, and a cell wall biogenesis-related gene, CCoAOMT1, were induced in salt-adapted A120 cells. Moreover, the loss-of-function mutant of Arabidopsis TUB9 gene, tub9, showed a hypersensitive phenotype to salt stress. Consistent overexpression of Arabidopsis TUB9 gene in rice transgenic plants enhanced tolerance to salt stress. Our results suggest that microtubules play crucial roles in plant adaptation and tolerance to salt stress. The modulation of microtubule-related gene expression can be an effective strategy for developing salt-tolerant crops. MDPI 2021-05-31 /pmc/articles/PMC8199277/ /pubmed/34073070 http://dx.doi.org/10.3390/ijms22115957 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chun, Hyun Jin
Baek, Dongwon
Jin, Byung Jun
Cho, Hyun Min
Park, Mi Suk
Lee, Su Hyeon
Lim, Lack Hyeon
Cha, Ye Jin
Bae, Dong-Won
Kim, Sun Tae
Yun, Dae-Jin
Kim, Min Chul
Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress
title Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress
title_full Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress
title_fullStr Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress
title_full_unstemmed Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress
title_short Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress
title_sort microtubule dynamics plays a vital role in plant adaptation and tolerance to salt stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199277/
https://www.ncbi.nlm.nih.gov/pubmed/34073070
http://dx.doi.org/10.3390/ijms22115957
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