Cargando…

Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides

Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously...

Descripción completa

Detalles Bibliográficos
Autores principales: Kasimova, Anastasiya A., Arbatsky, Nikolay P., Tickner, Jacob, Kenyon, Johanna J., Hall, Ruth M., Shneider, Michael M., Dzhaparova, Alina A., Shashkov, Alexander S., Chizhov, Alexander O., Popova, Anastasiya V., Knirel, Yuriy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199413/
https://www.ncbi.nlm.nih.gov/pubmed/34073255
http://dx.doi.org/10.3390/ijms22115641
_version_ 1783707371045912576
author Kasimova, Anastasiya A.
Arbatsky, Nikolay P.
Tickner, Jacob
Kenyon, Johanna J.
Hall, Ruth M.
Shneider, Michael M.
Dzhaparova, Alina A.
Shashkov, Alexander S.
Chizhov, Alexander O.
Popova, Anastasiya V.
Knirel, Yuriy A.
author_facet Kasimova, Anastasiya A.
Arbatsky, Nikolay P.
Tickner, Jacob
Kenyon, Johanna J.
Hall, Ruth M.
Shneider, Michael M.
Dzhaparova, Alina A.
Shashkov, Alexander S.
Chizhov, Alexander O.
Popova, Anastasiya V.
Knirel, Yuriy A.
author_sort Kasimova, Anastasiya A.
collection PubMed
description Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of l-rhamnose (l-Rhap) and 6-deoxy-l-talose (l-6dTalp). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) l-6dTalp-(1→3)-D-GlcpNAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes α l-Rhap side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different wzy gene is found in KL112. However, though both KL106 and KL112 share the atr8 acetyltransferase gene with KL83, only K83 is acetylated.
format Online
Article
Text
id pubmed-8199413
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81994132021-06-14 Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides Kasimova, Anastasiya A. Arbatsky, Nikolay P. Tickner, Jacob Kenyon, Johanna J. Hall, Ruth M. Shneider, Michael M. Dzhaparova, Alina A. Shashkov, Alexander S. Chizhov, Alexander O. Popova, Anastasiya V. Knirel, Yuriy A. Int J Mol Sci Article Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of l-rhamnose (l-Rhap) and 6-deoxy-l-talose (l-6dTalp). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) l-6dTalp-(1→3)-D-GlcpNAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes α l-Rhap side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different wzy gene is found in KL112. However, though both KL106 and KL112 share the atr8 acetyltransferase gene with KL83, only K83 is acetylated. MDPI 2021-05-26 /pmc/articles/PMC8199413/ /pubmed/34073255 http://dx.doi.org/10.3390/ijms22115641 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kasimova, Anastasiya A.
Arbatsky, Nikolay P.
Tickner, Jacob
Kenyon, Johanna J.
Hall, Ruth M.
Shneider, Michael M.
Dzhaparova, Alina A.
Shashkov, Alexander S.
Chizhov, Alexander O.
Popova, Anastasiya V.
Knirel, Yuriy A.
Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides
title Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides
title_full Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides
title_fullStr Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides
title_full_unstemmed Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides
title_short Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides
title_sort acinetobacter baumannii k106 and k112: two structurally and genetically related 6-deoxy-l-talose-containing capsular polysaccharides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199413/
https://www.ncbi.nlm.nih.gov/pubmed/34073255
http://dx.doi.org/10.3390/ijms22115641
work_keys_str_mv AT kasimovaanastasiyaa acinetobacterbaumanniik106andk112twostructurallyandgeneticallyrelated6deoxyltalosecontainingcapsularpolysaccharides
AT arbatskynikolayp acinetobacterbaumanniik106andk112twostructurallyandgeneticallyrelated6deoxyltalosecontainingcapsularpolysaccharides
AT ticknerjacob acinetobacterbaumanniik106andk112twostructurallyandgeneticallyrelated6deoxyltalosecontainingcapsularpolysaccharides
AT kenyonjohannaj acinetobacterbaumanniik106andk112twostructurallyandgeneticallyrelated6deoxyltalosecontainingcapsularpolysaccharides
AT hallruthm acinetobacterbaumanniik106andk112twostructurallyandgeneticallyrelated6deoxyltalosecontainingcapsularpolysaccharides
AT shneidermichaelm acinetobacterbaumanniik106andk112twostructurallyandgeneticallyrelated6deoxyltalosecontainingcapsularpolysaccharides
AT dzhaparovaalinaa acinetobacterbaumanniik106andk112twostructurallyandgeneticallyrelated6deoxyltalosecontainingcapsularpolysaccharides
AT shashkovalexanders acinetobacterbaumanniik106andk112twostructurallyandgeneticallyrelated6deoxyltalosecontainingcapsularpolysaccharides
AT chizhovalexandero acinetobacterbaumanniik106andk112twostructurallyandgeneticallyrelated6deoxyltalosecontainingcapsularpolysaccharides
AT popovaanastasiyav acinetobacterbaumanniik106andk112twostructurallyandgeneticallyrelated6deoxyltalosecontainingcapsularpolysaccharides
AT knirelyuriya acinetobacterbaumanniik106andk112twostructurallyandgeneticallyrelated6deoxyltalosecontainingcapsularpolysaccharides