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Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides
Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199413/ https://www.ncbi.nlm.nih.gov/pubmed/34073255 http://dx.doi.org/10.3390/ijms22115641 |
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author | Kasimova, Anastasiya A. Arbatsky, Nikolay P. Tickner, Jacob Kenyon, Johanna J. Hall, Ruth M. Shneider, Michael M. Dzhaparova, Alina A. Shashkov, Alexander S. Chizhov, Alexander O. Popova, Anastasiya V. Knirel, Yuriy A. |
author_facet | Kasimova, Anastasiya A. Arbatsky, Nikolay P. Tickner, Jacob Kenyon, Johanna J. Hall, Ruth M. Shneider, Michael M. Dzhaparova, Alina A. Shashkov, Alexander S. Chizhov, Alexander O. Popova, Anastasiya V. Knirel, Yuriy A. |
author_sort | Kasimova, Anastasiya A. |
collection | PubMed |
description | Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of l-rhamnose (l-Rhap) and 6-deoxy-l-talose (l-6dTalp). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) l-6dTalp-(1→3)-D-GlcpNAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes α l-Rhap side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different wzy gene is found in KL112. However, though both KL106 and KL112 share the atr8 acetyltransferase gene with KL83, only K83 is acetylated. |
format | Online Article Text |
id | pubmed-8199413 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81994132021-06-14 Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides Kasimova, Anastasiya A. Arbatsky, Nikolay P. Tickner, Jacob Kenyon, Johanna J. Hall, Ruth M. Shneider, Michael M. Dzhaparova, Alina A. Shashkov, Alexander S. Chizhov, Alexander O. Popova, Anastasiya V. Knirel, Yuriy A. Int J Mol Sci Article Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of l-rhamnose (l-Rhap) and 6-deoxy-l-talose (l-6dTalp). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) l-6dTalp-(1→3)-D-GlcpNAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes α l-Rhap side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different wzy gene is found in KL112. However, though both KL106 and KL112 share the atr8 acetyltransferase gene with KL83, only K83 is acetylated. MDPI 2021-05-26 /pmc/articles/PMC8199413/ /pubmed/34073255 http://dx.doi.org/10.3390/ijms22115641 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kasimova, Anastasiya A. Arbatsky, Nikolay P. Tickner, Jacob Kenyon, Johanna J. Hall, Ruth M. Shneider, Michael M. Dzhaparova, Alina A. Shashkov, Alexander S. Chizhov, Alexander O. Popova, Anastasiya V. Knirel, Yuriy A. Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides |
title | Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides |
title_full | Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides |
title_fullStr | Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides |
title_full_unstemmed | Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides |
title_short | Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides |
title_sort | acinetobacter baumannii k106 and k112: two structurally and genetically related 6-deoxy-l-talose-containing capsular polysaccharides |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199413/ https://www.ncbi.nlm.nih.gov/pubmed/34073255 http://dx.doi.org/10.3390/ijms22115641 |
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