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Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells
The aim of the work was to study the interaction between boron-rich boron carbide nanoparticles and selected tumor and immune phagocytic cells. Experiments were performed to investigate the feasibility of the application of boron carbide nanoparticles as a boron carrier in boron neutron capture ther...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199563/ https://www.ncbi.nlm.nih.gov/pubmed/34199341 http://dx.doi.org/10.3390/ma14113010 |
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author | Kozień, Dawid Szermer-Olearnik, Bożena Rapak, Andrzej Szczygieł, Agnieszka Anger-Góra, Natalia Boratyński, Janusz Pajtasz-Piasecka, Elżbieta Bućko, Mirosław M. Pędzich, Zbigniew |
author_facet | Kozień, Dawid Szermer-Olearnik, Bożena Rapak, Andrzej Szczygieł, Agnieszka Anger-Góra, Natalia Boratyński, Janusz Pajtasz-Piasecka, Elżbieta Bućko, Mirosław M. Pędzich, Zbigniew |
author_sort | Kozień, Dawid |
collection | PubMed |
description | The aim of the work was to study the interaction between boron-rich boron carbide nanoparticles and selected tumor and immune phagocytic cells. Experiments were performed to investigate the feasibility of the application of boron carbide nanoparticles as a boron carrier in boron neutron capture therapy. Boron carbide powder was prepared by the direct reaction between boron and soot using the transport of reagents through the gas phase. The powder was ground, and a population of nanoparticles with an average particle size about 80 nm was selected by centrifugation. The aqueous suspension of the nanoparticles was functionalized with human immunoglobulins or FITC-labeled human immunoglobulins and was then added to the MC38 murine colon carcinoma and to the RAW 264.7 cell line of mouse macrophages. Flow cytometry analysis was used to determine interactions between the functionalized boron carbide nanoparticles and respective cells. It was shown that B(4)C–IgG nanoconjugates may bind to phagocytic cells to be internalized by them, at least partially, whereas such nanoconjugates can only slightly interact with molecules on the cancer cells’ surface. |
format | Online Article Text |
id | pubmed-8199563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81995632021-06-14 Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells Kozień, Dawid Szermer-Olearnik, Bożena Rapak, Andrzej Szczygieł, Agnieszka Anger-Góra, Natalia Boratyński, Janusz Pajtasz-Piasecka, Elżbieta Bućko, Mirosław M. Pędzich, Zbigniew Materials (Basel) Article The aim of the work was to study the interaction between boron-rich boron carbide nanoparticles and selected tumor and immune phagocytic cells. Experiments were performed to investigate the feasibility of the application of boron carbide nanoparticles as a boron carrier in boron neutron capture therapy. Boron carbide powder was prepared by the direct reaction between boron and soot using the transport of reagents through the gas phase. The powder was ground, and a population of nanoparticles with an average particle size about 80 nm was selected by centrifugation. The aqueous suspension of the nanoparticles was functionalized with human immunoglobulins or FITC-labeled human immunoglobulins and was then added to the MC38 murine colon carcinoma and to the RAW 264.7 cell line of mouse macrophages. Flow cytometry analysis was used to determine interactions between the functionalized boron carbide nanoparticles and respective cells. It was shown that B(4)C–IgG nanoconjugates may bind to phagocytic cells to be internalized by them, at least partially, whereas such nanoconjugates can only slightly interact with molecules on the cancer cells’ surface. MDPI 2021-06-02 /pmc/articles/PMC8199563/ /pubmed/34199341 http://dx.doi.org/10.3390/ma14113010 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kozień, Dawid Szermer-Olearnik, Bożena Rapak, Andrzej Szczygieł, Agnieszka Anger-Góra, Natalia Boratyński, Janusz Pajtasz-Piasecka, Elżbieta Bućko, Mirosław M. Pędzich, Zbigniew Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells |
title | Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells |
title_full | Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells |
title_fullStr | Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells |
title_full_unstemmed | Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells |
title_short | Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells |
title_sort | boron-rich boron carbide nanoparticles as a carrier in boron neutron capture therapy: their influence on tumor and immune phagocytic cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199563/ https://www.ncbi.nlm.nih.gov/pubmed/34199341 http://dx.doi.org/10.3390/ma14113010 |
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