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Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells

The aim of the work was to study the interaction between boron-rich boron carbide nanoparticles and selected tumor and immune phagocytic cells. Experiments were performed to investigate the feasibility of the application of boron carbide nanoparticles as a boron carrier in boron neutron capture ther...

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Autores principales: Kozień, Dawid, Szermer-Olearnik, Bożena, Rapak, Andrzej, Szczygieł, Agnieszka, Anger-Góra, Natalia, Boratyński, Janusz, Pajtasz-Piasecka, Elżbieta, Bućko, Mirosław M., Pędzich, Zbigniew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199563/
https://www.ncbi.nlm.nih.gov/pubmed/34199341
http://dx.doi.org/10.3390/ma14113010
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author Kozień, Dawid
Szermer-Olearnik, Bożena
Rapak, Andrzej
Szczygieł, Agnieszka
Anger-Góra, Natalia
Boratyński, Janusz
Pajtasz-Piasecka, Elżbieta
Bućko, Mirosław M.
Pędzich, Zbigniew
author_facet Kozień, Dawid
Szermer-Olearnik, Bożena
Rapak, Andrzej
Szczygieł, Agnieszka
Anger-Góra, Natalia
Boratyński, Janusz
Pajtasz-Piasecka, Elżbieta
Bućko, Mirosław M.
Pędzich, Zbigniew
author_sort Kozień, Dawid
collection PubMed
description The aim of the work was to study the interaction between boron-rich boron carbide nanoparticles and selected tumor and immune phagocytic cells. Experiments were performed to investigate the feasibility of the application of boron carbide nanoparticles as a boron carrier in boron neutron capture therapy. Boron carbide powder was prepared by the direct reaction between boron and soot using the transport of reagents through the gas phase. The powder was ground, and a population of nanoparticles with an average particle size about 80 nm was selected by centrifugation. The aqueous suspension of the nanoparticles was functionalized with human immunoglobulins or FITC-labeled human immunoglobulins and was then added to the MC38 murine colon carcinoma and to the RAW 264.7 cell line of mouse macrophages. Flow cytometry analysis was used to determine interactions between the functionalized boron carbide nanoparticles and respective cells. It was shown that B(4)C–IgG nanoconjugates may bind to phagocytic cells to be internalized by them, at least partially, whereas such nanoconjugates can only slightly interact with molecules on the cancer cells’ surface.
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spelling pubmed-81995632021-06-14 Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells Kozień, Dawid Szermer-Olearnik, Bożena Rapak, Andrzej Szczygieł, Agnieszka Anger-Góra, Natalia Boratyński, Janusz Pajtasz-Piasecka, Elżbieta Bućko, Mirosław M. Pędzich, Zbigniew Materials (Basel) Article The aim of the work was to study the interaction between boron-rich boron carbide nanoparticles and selected tumor and immune phagocytic cells. Experiments were performed to investigate the feasibility of the application of boron carbide nanoparticles as a boron carrier in boron neutron capture therapy. Boron carbide powder was prepared by the direct reaction between boron and soot using the transport of reagents through the gas phase. The powder was ground, and a population of nanoparticles with an average particle size about 80 nm was selected by centrifugation. The aqueous suspension of the nanoparticles was functionalized with human immunoglobulins or FITC-labeled human immunoglobulins and was then added to the MC38 murine colon carcinoma and to the RAW 264.7 cell line of mouse macrophages. Flow cytometry analysis was used to determine interactions between the functionalized boron carbide nanoparticles and respective cells. It was shown that B(4)C–IgG nanoconjugates may bind to phagocytic cells to be internalized by them, at least partially, whereas such nanoconjugates can only slightly interact with molecules on the cancer cells’ surface. MDPI 2021-06-02 /pmc/articles/PMC8199563/ /pubmed/34199341 http://dx.doi.org/10.3390/ma14113010 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kozień, Dawid
Szermer-Olearnik, Bożena
Rapak, Andrzej
Szczygieł, Agnieszka
Anger-Góra, Natalia
Boratyński, Janusz
Pajtasz-Piasecka, Elżbieta
Bućko, Mirosław M.
Pędzich, Zbigniew
Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells
title Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells
title_full Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells
title_fullStr Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells
title_full_unstemmed Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells
title_short Boron-Rich Boron Carbide Nanoparticles as a Carrier in Boron Neutron Capture Therapy: Their Influence on Tumor and Immune Phagocytic Cells
title_sort boron-rich boron carbide nanoparticles as a carrier in boron neutron capture therapy: their influence on tumor and immune phagocytic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199563/
https://www.ncbi.nlm.nih.gov/pubmed/34199341
http://dx.doi.org/10.3390/ma14113010
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